We narrowed to 793 results for: KIN
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TypeBlog PostPublishedJune 29, 2023, 7 p.m....video. Don't worry - no pipettes were harmed in the making of this photo! Here, I was also given a mixture...such as stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory... my time here comes to an end, my worries about making connections with coworkers seem, in retrospect,...
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Fostering communication in remote collaborations
TypeBlog PostPublishedApril 21, 2020, 1:14 p.m....Especially when your collaboration includes scientists working in different time zones, it’s important that everyone...accountable to these expectations once they’re set by checking in about them at your recurring meetings and reaching...common. I hope these tips are useful whether you’re looking to improve communication within an existing collaboration... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m....headquarters. I decided to go ahead and apply; thinking I probably would not get selected for both the...compose and submit a manuscript that they were working on currently. Upon arrival to the headquarters,... The most beneficial aspect of this course was breaking up into focus groups with a facilitator. In the... -
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....expressing a selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and ...to be assayed (e.g. pPD95_86 and pPD57_56), or knocking down YGOI through RNA-mediated interference (e.g... applications used to study gene function. From making transgenic animals, gene targeting and genome modifications... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m.... used at high concentrations (and vice versa). Looking around the lab, you'll likely find many of the ...mammalian cells. Name Class Mode of Action* Working Concentration** Kanamycin aminoglycoside Binds...their mechanism for killing bacteria, and general working concentrations. For instructions on how to prepare... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....fewer ethical and logistical challenges than does working with animals. Because Arabidopsis is small, has... to grow, it is an ideal organism for teachers looking to expand their science curriculum. Growing Arabidopsis... biology teacher or professor? Whether you are looking to develop a new teaching resource or you want ... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....replication machinery room to come in and get busy making copies. Types of origins of replication ...compatibility with one or more other plasmids. Generally speaking, plasmids with the same ori are incompatible because...chloramphenicol – make sure your growth conditions aren't working against you! The culture inoculum Freshly ... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog PostPublishedSept. 17, 2020, 1:15 p.m....Addgene started dealing with this new reality of working remotely, scientists around the world mobilized...took to make all this happen with 80% of the team working remotely. We’re extremely proud of the work we’... maintain our workforce for when we need to be working at full capacity to serve the scientific community... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....from Zhu et al., 2019. Wensheng Wei's lab at Peking University has developed a new CRISPR screening...injection of precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive...fluorescing green. Image from Shin et al., 2019. Looking for an alternative to intercalating fluorescent... -
Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....around the world who would otherwise have difficulty making or getting these reagents is profound.” Frank Stegmeier...Library, a library that “enables the high-resolution tracking of more than 1 million cancer cells under drug...became passionate about virology,” he says. He was working in an HIV lab and based on that experience, soon...