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We narrowed to 798 results for: KIN

Showing: 391 - 400 of 798 results
  1. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...background noise. This is not terribly problematic when working with cell culture, because it is easy to harvest...harvest large quantities of cells. However, when working on specific tissues or organs in whole animal models...major technical hurdle.   ChIP-seq requires  cross-linking during an initial fixation step. This serves to...
  2. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...Especially when your collaboration includes scientists working in different time zones, it’s important that everyone...accountable to these expectations once they’re set by checking in about them at your recurring meetings and reaching...common. I hope these tips are useful whether you’re looking to improve communication within an existing collaboration...
  3. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ... cells containing the plasmid construct you’re seeking. Fortunately, there are many ways to do this involving... a successful uptake of your DNA of interest by taking advantage of bacterial characteristics such as ...for specific amino acids. When plated on media lacking the essential amino acid, the bacteria will only...
  4. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...also involved in reinforcement learning, decision making, and motor control.  What do we understand about... 100 nm) of DA. In addition, they exhibit fast kinetics (10 ms on and 100 ms off), enabling detection ...magnitudes of fluorescent responses. Studies on the kinetics of these sensors showed rapid response (60 or ...
  5. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...headquarters. I decided to go ahead and apply; thinking I probably would not get selected for both the...compose and submit a manuscript that they were working on currently. Upon arrival to the headquarters,... The most beneficial aspect of this course was breaking up into focus groups with a facilitator. In the...
  6. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...expressing a selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and ...to be assayed (e.g. pPD95_86 and pPD57_56), or knocking down YGOI through RNA-mediated interference (e.g... applications used to study gene function. From making transgenic animals, gene targeting and genome modifications...
  7. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ... used at high concentrations (and vice versa). Looking around the lab, you'll likely find many of the ...mammalian cells.   Name Class Mode of Action*   Working Concentration** Kanamycin aminoglycoside Binds...their mechanism for killing bacteria, and general working concentrations. For instructions on how to prepare...
  8. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...frequently with little notice or choice Always working on a team/in a matrix - can be frustrating for ...but good performers move around easily  Table 1: Taking it a Step Further - Big Company v. Small Company...on what paths to pursue. Spend some time really thinking about your motivations and what parts of being...
  9. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...Addgene started dealing with this new reality of working remotely, scientists around the world mobilized...took to make all this happen with 80% of the team working remotely. We’re extremely proud of the work we’... maintain our workforce for when we need to be working at full capacity to serve the scientific community...
Showing: 391 - 400 of 798 results