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Showing: 781 - 800 of 889 results
  1. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...this system was optimized for Burkholderia, they identified 67 genera of Proteobacteria that this toolkit...sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Find recently deposited...
  2. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...wall of green algae (Soo et al., 2015). There, it divides into two cells. At the same time, it sucks out ...Spinochordodes tellinii‘s larvae makes its home inside grasshoppers and crickets. When it’s ready to transform...
  3. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ... own unique protein fusions. This collection is ideal for tandem affinity purification of protein complexes... describe a modular collection of plasmids for a wide variety of genome modifications in S. Pombe, including...
  4. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...antagonist. This is important because alpha2-AR can provide inhibitory feedback in response to norepinephrine...Optogenetics Plasmids Visit the Fluorescent Protein Guide Pages ...
  5. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...dramatically increase the chances of being seriously considered for an open position.  Check out Joanne's Reddit...network your way to a hiring manager. Learn to confidently describe your strengths in your resume and don't...
  6. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...Boston University last week.  This fantastic city-wide effort was led by two amazing postdocs, Kristin ...about academia and alternate training needs to be provided to take advantage of all the possible science ...
  7. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...way to share their plasmids. Now I am on the other side of the force and as an Addgenie I will do all my...scientific discovery or invention, and sharing those ideas with others. This love of interacting with people...
  8. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...back from other summer programs. Amy took into consideration just how busy I was and ensured that my decision...concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running on a time crunch...
  9. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...
  10. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...from the labs of Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins...KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that...
  11. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...well-known reward chemical and neurotransmitter. But aside from DA’s famous role as the “happy hormone,” it...Addgene's viral service Find viral protocols and videos Find biosensor AAVs for your research ...
  12. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...occurring AAV serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the ...type tropism. Introducing changes and/or short peptides sequences into these variable loops is one method...
  13. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...nuclease free water is a great option because it provides a great versatility on how to use your plasmid...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...
  14. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...the SPARC system, attP sites are located on either side of a stop cassette, upstream of an effector gene...Cell-type-specific and projection-specific brain-wide reconstruction of single neurons. Nature Methods...
  15. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...Intelligence or to hone delegation skills, for example. So aside from a focus on removing obstacles, what makes a... (this actually varies, a manager does best to identify the work style of each team member) Need challenging...
  16. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...Bundling up for winter? The weather outside might be frightful, but browsing the 49 new preps available... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers...
  17. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...primary antibodies can be expensive and may not provide enough signal to visualize low-abundance targets...your primary and secondary antibodies as normal. Ideally, your secondary will only bind to your primary ...
Showing: 781 - 800 of 889 results