We narrowed to 811 results for: cha

1. Rabies and Neuronal Tracing

   Type

   Blog Post

   Published

   May 29, 2018, 1:51 p.m.

   ...Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics of a G-deficient rabies virus recombinant: ...

2. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   Published

   Oct. 22, 2019, 12:20 p.m.

   ... Michael G. Lemieux...

3. Mouse Modeling, Part 2: Breeding and Crossing Mice

   Type

   Blog Post

   Published

   Aug. 6, 2019, 12:55 p.m.

   ... number or expression level effects.  Another challenge that may arise when crossing mice with multiple...

4. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   Published

   Aug. 9, 2022, 1:15 p.m.

   ...application (different experiments/conditions can change binding ability – i.e., the antibody can no longer...

5. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ...Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa, CRISPRi, editing)   Figure...

6. High Complexity Golden Gate Assembly with NEB’s DAD

   Type

   Blog Post

   Published

   Aug. 1, 2023, 1:15 p.m.

   ...already-existing sets. Note that this tool relies on a stochastic search algorithm rather than pre-designed sets...

7. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   ...familiarize yourself with your organization’s reporting mechanisms before you start an experiment, just in case ...

8. Hot Plasmids and Viral Preps - March 2021

   Type

   Blog Post

   Published

   March 23, 2021, 1:15 p.m.

   ...repression, and editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence...

9. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

   Type

   Blog Post

   Published

   Dec. 1, 2015, 3:30 p.m.

   ...No conference would be complete without CRISPR Michael Terns from of the University of Georgia studies...

10. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...

11. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    Published

    July 13, 2017, 2:30 p.m.

    ... genetic redundancy (meaning, there’s more of a chance of seeing a phenotype since additional genes that...

12. Plasmids 101: Degron Tags

    Type

    Blog Post

    Published

    March 14, 2024, 1:15 p.m.

    ...CRISPR, siRNA, etc. Firstly, they are reversible mechanisms of control. Protein degradation can be induced...

13. Google Forums Round Up: First Impressions of NgAgo

    Type

    Blog Post

    Published

    Aug. 4, 2016, 2:30 p.m.

    ...in house using a simple PNK reaction or can be purchased 5’-modified from a company. However, unlike an...

14. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    Published

    Feb. 4, 2016, 3:30 p.m.

    ...The backbone modifications listed can refer to changes made to the original backbone by the depositing...

15. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...model.  If your expression system is not well characterized in terms of CRISPR use, you will want to invest...

16. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...this type of experiment can shed light on the mechanism by which cells gain resistance to drugs and can...

17. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ...enjoy seeing how our quality control process has changed with the introduction of next generation sequencing...

18. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ... like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While the pre-existing...

19. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ...domains of a protein that undergoes conformational change. Typically, two fluorescent proteins with overlapping...

20. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...- since they frequently undergo conformational changes after target binding, aptamers are highly customizable...