We narrowed to 875 results for: ica
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TypeBlog PostPublishedJan. 23, 2014, 3:23 p.m....cassettes onto a single T-DNA in subsequent rounds, dramatically reducing the time required to generate multi-construct...multi-construct transgenic plants. In the most practical terms, what GreenGate can do is “save you half a...
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The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....The GCaMP5 and GCaMP6 series of genetically encoded calcium indicators were the hot technology of the ... -
Top 10 Open Science Developments of 2013
TypeBlog PostPublishedDec. 19, 2013, 3:46 p.m....promote open science, ensure good stewardship of clinical trial data, serve society and patients, and respect...support for open access to peer-reviewed journal publications, although many don’t necessarily publish in ... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog PostPublishedJuly 21, 2015, 2:30 p.m....determining meaningful solutions. PI-trainee communication builds a positive lab culture Although they ... communication... -
Celebrating Accomplishments in the Lab
TypeBlog PostPublishedMay 8, 2014, 3:07 p.m.... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog PostPublishedJan. 2, 2014, 3:44 p.m....notebooks and binder on your desk. Arrange them in chronological order (or by project, if that works better for... notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to... -
Supporting Rare Disease Research with BeHeard 2017
TypeBlog PostPublishedJuly 20, 2017, 2:30 p.m....Institute partners with companies to provide technological resources for those studying rare disease. This... University of British Columbia Department of Urological Sciences. Dr. Nabavi studies peritoneal mesothelioma... -
What's New in CRISPR - September 2019
TypeBlog PostPublishedSept. 10, 2019, 12:56 p.m....plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...toxicity of Cas9. Read the paper in Nature Communications Find the Bikard E. coli Genome-wide Inhibition... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog PostPublishedJan. 23, 2024, 2:15 p.m..... Through an expansion of our export license application service, we’re pleased to announce that we are...benefited from the business rigor and efficiencies typically adopted by for-profit companies, while making ... -
A Better Way to Get Customer Support: The Help Center
TypeBlog PostPublishedDec. 10, 2015, 3:30 p.m.... we have a dedicated support team that can help you through any search, ordering, technical, or quality... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m.... their materials. The new office in London is dedicated to helping them find their way to new scientific... then she has worked in the the UK, California, Africa, and Australia, viewing science as a global collaboration... -
Build Your CRISPR Vocabulary
TypeBlog PostPublishedJuly 2, 2024, 1:15 p.m....CRISPR array. The CRISPR array is composed of identical repeat sequences and variable spacer sequences...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable... -
Management for Scientists: Managing vs. Leading
TypeBlog PostPublishedMarch 31, 2015, 4:08 p.m....Their work involves coordination and can require technical mastery. Scientists need to be doers for a long...in the way of the doers and by ensuring good communication. A manager must both hire and fire to make sure... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m....be a time when you need to speak publicly to communicate your work. One of my 10 tips for giving a great...the board of a professional society, solved a technical problem, had an alter ego as a mad scientist for... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....contains the proteins needed for the virus to replicate, and the transfer plasmid which contains the ... sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ITRs due to their secondary... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....Therapy. Existing methods for AAV quality control Historically, AAV vector QC has been sparse including only...that could be used as a template for downstream applications (Berns, 1972). Using qPCR and restriction enzyme... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m....of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....gene encodes a sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they...models carrying different SOX10 mutations. More specifically, they’ve proposed to use plasmids with modified... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....positions posted on recruitment websites, this job specifically looked for a researcher with 3 years+ of cancer...on the marketing or business background of the applicant. However, although this position did look for ... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m....Christopher Harvey’s lab. According to their publication (Chettih & Harvey, 2019), “this localization ...Recombinase AAV Targeting and manipulating specific, genetically-defined neuronal populations with intersectional...