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We narrowed to 892 results for: CAN

Showing: 401 - 410 of 892 results

  1. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...light. PA-FPs can be activated from low fluorescent states to high fluorescence states, they can change from...fluorescent color to another, or their fluorescence can be switched on and off reversibly. This ability to...Irreversible photoactivation Irreversible PA-FPs can be switched on from a state of dim or no fluorescence...photoconvertible fluorescent proteins (PC-FPs). PC-FPs can convert from one fluorescent color to another (e.... is another monomeric PA-FP which unlike mEosFP, can form it’s chromophore at a more comfortable 37°C ...reversible PA-FPs (also known as photoswitchers) can be photoswitched from a dark state to a bright fluorescent...non-fluorescent state (“quenching”). The photoswitch can occur multiple times by activating with two distinct...

  2. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...base-to-base conversions (Anzalone et al., 2019). Plus, it can combine different types of edits with one another...extends the reach of CRISPR genome editing, as it can edit near to or far from PAM sites, making it less...availability than other methods. The PAM-to-edit distance can be over 30 base pairs for prime editing. Since PAM... (in certain circumstances) Current base editors can perform any base-to-base conversion in principle,...require multiple steps and enzymes. Prime editing can encode any of the 12 possible base-to-base changes...bystander editing is unacceptable, prime editors can be used to avoid this possibility. However, there... strategy, cell type, and goal of the experiment can all impose different constraints and affect how you...

  3. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...about how treatment regimens can be improved and how the therapies themselves can be made more effective. ...future immune surveillance. Dormancy of certain cancers can be attributed to the concept of immunoediting...equilibrium stage. This can be called “stalemate equilibrium” where the cancer stops growing, and the ...that eliminate early stage cancer cells, chronic inflammatory responses can augment tumor growth. Indeed...inflammation from infection can further divert the immune system from fighting cancer. Surgical removal causes...treatment strategies can be enhanced. Types of immunotherapeutics In lieu of the cancer-immune system interactions...because they can help start a sequence of immune reactions that aid in the fight against cancer. Sipuleucel-T...

  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...cases, counter-selection escape can occur: single crossover colonies can acquire the ability to grow on...multiple transformation and plasmid curing steps, and can generate off-target mutations. Modifying bacterial...alternative, endogenous homologous recombination enzymes can also accomplish the desired genomic modification....crossover”) which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance...on sucrose will identify double crossovers. This can be an efficient method to generate precise, unmarked... because in these circumstances, the conjugation can be so inefficient, that instead of the resulting ...the scarless modification of bacterial genomes. It can accomplish large deletions, insertions, and even ...

  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...or A much further from the ‘NGG’ PAM cannot be editable? They can, but they require other base editor ...about CRISPR, one question probably comes to mind: can CRISPR be applied to fix the defective base precisely...CRISPR-mediated base editing (BE), these types of repairs can now be done more efficiently than before. A base ...guanine (G)? You may wonder if a CRISPR-base editor can perform this change. However, there are no naturally...successful evolution of an adenosine deaminase that can act on DNA for adenine base editing. They first created...pKABE6.3, pKABE7.9, and pKABE7.10) so that the user can optimize base editing efficiency based on the location...to the protospacer adjacent motif (PAM). ABE7.10 can edit target 'A' if it lies in a small genomic window...

  6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...working integrase, IDLVs can still deliver their genetic payload to the cell but cannot integrate it into the...tools for manipulating cells and organisms. They can be used for a variety of research purposes, like ...cells, among many other uses. Lentiviral vectors can be produced easily, without the need for time-consuming...is then part of the cellular DNA… forever!  This can be an advantage for researchers looking to generate... for integration, two types of circular episomes can be generated through either homologous recombination...expression of Cas9 is no longer necessary. However, Cas9 can be toxic if continuously expressed (Fu et al., 2013...3. Stable expression in non-dividing cells IDLVs can also be useful for transducing non-dividing cells...

  7. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...master mix of enzymes can be purchased from a company (e.g. NEB or SGI-DNA), or can be mixed yourself (e.g...easy assembly of multiple linear DNA fragments, but can also be used in basic cloning of an insert into your...great part about this mix of enzymes is that they can all work at the same temperature, so the entire reaction...Miller Lab Protocol). The Gibson assembly process can be used to assemble up to 6 fragments in one step... required homology between neighboring fragments can be created via PCR amplification with primers that...fragments. However, by using Gibson assembly, you can insert both the gene of interest and the tag sequences... Excluding the time it takes to get primers, you can have your construct made in 5 days. One drawback...

  8. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...cloning (discussed below) can run on the order of $20-30 per reaction but can be used to quickly generate...fragments in a prescribed order. These fragments can be pooled in one tube and ligated in as little as...needed for this method is relatively inexpensive and can be obtained for about $70, resulting in a price of...dollars per reaction. By comparison, Gibson assembly can cost over $15.00 per reaction if you purchase a pre-made...remember that commercially available cloning kits can often make the process much more streamlined, because...homology based methods, like Gibson or SLIC, since one can pool the components and define their final order ...

  9. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...blue. Students can create synthetic riboprobes off of linearized DNA templates which they can easily generate...differ. For example, Girardia sp. and Dugesia dorotocephalacan can be fed dsRNA-expressing E. coli that are...version of L440. This vector is versatile in that it can be used for both in situ hybridization and dsRNA-...bidirectional transcription to create dsRNA. Students can produce DNA templates via PCR off of the same plasmid...comparison. Performing WISH using these plasmids can result in  the labeling specific cell types or entire...number of genes that other classrooms and researchers can easily investigate in planarians. References 1. ...Kimberly C. Tu, Alejandro Sánchez Alvarado, The American Biology Teacher, Vol. 79 No. 3, March 2017; (pp...

  10. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...researcher can cause them to interact by stimulation with blue light.  Similarly, light can also be used...further probe cellular function. The CRY2clust Plasmid can be found here. Park et al. Nat Commun. 2017. PubMed...“addicted” E. coli strain expressing this operon can serve as a living biosensor for measuring caffeine...pDCAF3 operon, the growth of this knockout strain can be rescued by the xanthine produced via complete ...organization. The design use to detect PKA activity can be generalized to measure phosphatase activity, second...proteins have improved photophysical properties and can be used in quenching, FRET (Förster resonance energy...
Showing: 401 - 410 of 892 results