We narrowed to 904 results for: CAN

...and sgRNAs can be found here. Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered...However, using CRISPRa to target individual genes can be relatively laborious because multiple plasmids...expression. Constitutive or inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing...distinct transcriptional start site. Because CRISPRa can be targeted anywhere in the genome, it is relatively...BDNF protein. To demonstrate the specificity that can be achieved with CRISPRa, the Day lab singled out...The Day Lab’s neuron-optimized lentiviral vectors can be found here. High-throughput screening strategies...used by the Qi Lab for this particular application can be found here.  Why CRISPRa? Although cDNA overexpression...

...senior faculty members who can help you identify overly ambitious goals and can help you distinguish mechanistic...question, the methods, the tools or techniques that can help resolve the question, the anticipated outcomes...the validity of a single model. The first approach can lead to new directions, while the second approach...-like mini-organs or organoids, neither of these can substitute for a liver transplant. My lab will combine...problem. Creating a coherent unified story and model can help make your ideas easier to understand and more...outside your lab and outside your field. If they can understand and are engaged by your presentation, ... getting back to your story. Committee questions can derail your talk. You need to balance respectfully...

...with the addition of N-glycans [9]. Both of these posttranslational modifications can disrupt FP folding ...fluoresce. A dark population is not readily apparent and can confound quantitative imaging experiments or even...susceptible to these non-native modifications, which can all potentially affect function [5]. We and others...cellular function or possibly viability. FP misfolding can even disrupt fusion protein localization. For example...pathway, a dark pool that is mislocalized to the ER can be detected with immunofluorescence. This population...experiments, please consider the following: How can one ensure that an FP fusion with a non-cytosolic...inert green, cyan, yellow, and blue variants that can be used in various combinations. For example, with...

...to DNA extraction and quantification so that you can focus on what you want to do with your plasmid without...starting with 210 plasmids from the collection. You can find the full list here. Most of these plasmids are...cloning. Plus, we decided to include 10 plasmids that can be directly used in the research on the SARS-CoV-...out this service! Let us know what you think. You can also let us know which plasmids you’d like to see...

...role in maintaining cellular function. Mitochondria can also lend their power to other cells when required... have shown that these transplanted mitochondria can exert their powers for better or for worse. To better...for MitoTRACER, as it is a diverse tool that can be used in many different systems, both in vitro and in...al. (2006). Mitochondrial transfer between cells can rescue aerobic respiration. Proceedings of the National...condensates are membraneless liquid "droplets" that can concentrate and sequester specific macromolecules...selectively perturb any kind of condensate. The killswitch can be genetically fused to a protein of interest or ...micropipettes, and plate readers. Instead, students can use inoculation loops for liquid transfer, incubate...

... you.” “Also a mentor can give you realistic expectations. (Don't think you can finish that book in 4 ..."The greatest good you can do for another is not just to share your riches but to reveal to him his own...experience. Navigating a fulfilling career in science can be challenging – is advice and guidance from only...Advisor/PI who will also be a good mentor? Where can I find a Mentor? What is this I hear about “group...

...light. PA-FPs can be activated from low fluorescent states to high fluorescence states, they can change from...fluorescent color to another, or their fluorescence can be switched on and off reversibly. This ability to...Irreversible photoactivation Irreversible PA-FPs can be switched on from a state of dim or no fluorescence...photoconvertible fluorescent proteins (PC-FPs). PC-FPs can convert from one fluorescent color to another (e.... is another monomeric PA-FP which unlike mEosFP, can form it’s chromophore at a more comfortable 37°C ...reversible PA-FPs (also known as photoswitchers) can be photoswitched from a dark state to a bright fluorescent...non-fluorescent state (“quenching”). The photoswitch can occur multiple times by activating with two distinct...

...base-to-base conversions (Anzalone et al., 2019). Plus, it can combine different types of edits with one another...extends the reach of CRISPR genome editing, as it can edit near to or far from PAM sites, making it less...availability than other methods. The PAM-to-edit distance can be over 30 base pairs for prime editing. Since PAM... (in certain circumstances) Current base editors can perform any base-to-base conversion in principle,...require multiple steps and enzymes. Prime editing can encode any of the 12 possible base-to-base changes...bystander editing is unacceptable, prime editors can be used to avoid this possibility. However, there... strategy, cell type, and goal of the experiment can all impose different constraints and affect how you...

...about how treatment regimens can be improved and how the therapies themselves can be made more effective. ...future immune surveillance. Dormancy of certain cancers can be attributed to the concept of immunoediting...equilibrium stage. This can be called “stalemate equilibrium” where the cancer stops growing, and the ...that eliminate early stage cancer cells, chronic inflammatory responses can augment tumor growth. Indeed...inflammation from infection can further divert the immune system from fighting cancer. Surgical removal causes...treatment strategies can be enhanced. Types of immunotherapeutics In lieu of the cancer-immune system interactions...because they can help start a sequence of immune reactions that aid in the fight against cancer. Sipuleucel-T...

...working integrase, IDLVs can still deliver their genetic payload to the cell but cannot integrate it into the...tools for manipulating cells and organisms. They can be used for a variety of research purposes, like ...cells, among many other uses. Lentiviral vectors can be produced easily, without the need for time-consuming...is then part of the cellular DNA… forever!  This can be an advantage for researchers looking to generate... for integration, two types of circular episomes can be generated through either homologous recombination...expression of Cas9 is no longer necessary. However, Cas9 can be toxic if continuously expressed (Fu et al., 2013...3. Stable expression in non-dividing cells IDLVs can also be useful for transducing non-dividing cells...