...new green glowing protein could be used to measure gene transcription, as well as to track protein localization... he was right! He began to study the A. victoria gene responsible for encoding GFP, and, in 1992, he reported...

...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the...treat disease through tissue repair, stem cells, gene therapy, and tissue engineering. How the multidisciplinary...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Darrell Kotton is the director of the Center for Regenerative Medicine at Boston University Medical School...source biology in his own lab, at the Center for Regenerative Medicine, and within the biomedical research...Along these lines, Darrell recently joined the Addgene Board of Directors where he hopes to help keep ... Kotton and the Boston University Center for Regenerative Medicine. Darrell describes his career goals...

...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian...way to visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented...loads of inspiration for the current and the next generation of microbiologists. If you got inspired to produce...PubMed Central PMCID: PMC5920530.  Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial...Science Communication using Twitter  Resources on Addgene.org Check out Our Microbiology Resource Page CRISPR...bacteria Deposit Your Microbiology Plasmids with Addgene ...

... this plasmid will work for them. Species of Gene or Insert Choose from: H. sapiens (human), M. ... Cloning Method Choose from: Gateway cloning, gene synthesis, Gibson cloning, ligation-independent ... Stable strain. For plasmids containing the ccdB gene, such as Gateway vectors, choose the ccdB Survival...information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene  Quality control... field, and contact Addgene. We may require that you send your samples to Addgene pre-transformed in this...the spreadsheet to deposit@addgene.org with the following information: Addgene account username. In order... to appear in your Addgene account under My Plasmids, we need to know your Addgene account username. Shipping...

...overexpressing a gene or protein after transfection). Future of FRET The first genetically encoded FRET biosensor...Resource page and can be used to create fusions with a gene of interest. These plasmids will be most useful ...designed to measure specific small biomolecules or gene activity are often intramolecular probes, as the...name a few.   Find Many FRET-Based Biosensors on Addgene's Biosensor Pages   Recent innovations have demonstrated...PubMed PMID: 26210398. Additional Resources on the Addgene Blog Tips for using FRET in Your Experiments Learn...FRET Based Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein...

...Plasmids for endogenous gene tagging in human cells Resources on Addgene.org Browse all CRISPR plasmids...endogenous ENL was inactivated using CRISPR/Cas9 gene-editing. Featured molecules: dTAG-7 and dTAG-13 ...endogenous MELK was inactivated using CRISPR/Cas9 gene-editing. Featured molecules: dTAG-7, dTAG-13, and...Figure 1). Plasmids: We generated a series of plasmids that are available from Addgene. These plasmids enable...47 Additional Resources on the Addgene Blog Easi-CRISPR: Generating Knock-In and Conditional Mouse Models.... To overcome this limitation, we created a generalizable tag-based strategy, which we refer to as the... scales that are not possible with traditional genetic approaches. In addition, the dTAG system is versatile...

...disease-causing alleles, and the growing need for gene editing in research, the old PAM constraints just...RNA will do. Similarly, if you want to knock out a gene, a frameshift mutation introduced anywhere within...take a bp or two upstream or downstream) for most gene targeting experiments. Between SpRY and several ...Resources on Addgene.org Addgene’s CRISPR Guide CRISPR Plasmids and Resources Resources on the Addgene blog ...biology, the answer is always it depends, but generally the efficiency of the desired edit is reduced ... Cas9s that recognize NG PAMs. The Nureki lab generated SpCas9-NG, a rationally engineered Cas9 with NG...name implies (Nishimasu et. al, 2018). xCas9 was generated by the Liu lab through phage-assisted evolution...

... bright enough to detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc...Fusion Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available...such as the dynamics of subcellular structures and gene expression. You must see the movies demonstrating...al. (2016) CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nature...when employing optogenetic regulators to initiate the event being monitored. Optogenetic regulators respond...wavelengths overlap with optogenetic initiation wavelengths, excitation of the optogenetic regulator leads to... be paired with optogenetic regulators. Many of these tools are available from Addgene and we're excited...

...address this issue by developing generic strategies for detecting cloned genes (Munro and Pelham, 1984). The...the human c-Myc gene (Evan, et al. 1985). Myc was one of the earliest identified oncogenes and was a target...tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the.... However, it was (and still is) infeasible to generate highly specific antibodies for every potential...explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic peptides corresponding...epitope within that region. You’ll notice that “generate a reliable antibody for protein tagging approaches...

...library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression is induced by...a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus... Figure 1. (A) SARS-CoV-2 RBD DMS Library generation and sequencing. From Starr et al Cell., 2020....scFV library and characterized them by ELISA, homogeneous time resolved fluorescence, and suspension bead...synthetase complex, the team investigated whether the generated antibodies could bind these aaRSs. They found ... Gräslund lab plans to continue their work by generating antibodies for additional aminoacyl tRNA synthetases...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...