We narrowed to 798 results for: KIN

...from Zhu et al., 2019.  Wensheng Wei's lab at Peking University has developed a new CRISPR screening...injection of precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive...fluorescing green. Image from Shin et al., 2019. Looking for an alternative to intercalating fluorescent...

...around the world who would otherwise have difficulty making or getting these reagents is profound.” Frank Stegmeier...Library, a library that “enables the high-resolution tracking of more than 1 million cancer cells under drug...became passionate about virology,” he says. He was working in an HIV lab and based on that experience, soon...

...replication machinery room to come in and get busy making copies.     Types of origins of replication ...compatibility with one or more other plasmids. Generally speaking, plasmids with the same ori are incompatible because...chloramphenicol – make sure your growth conditions aren't working against you!  The culture inoculum Freshly ...

...species). This feature is advantageous if you’re working with small capacity viral vectors such as AAV. ... “recovering” its original sequence. If you’re looking to disrupt gene function via indel formation, this...targets. Are you using NgAgo? Let us know how it’s working in the comments section! Read About NgAgo User'...

...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC... get PCR amplification. We can start similarly, taking the final 18 bases of the ORF, including the stop...

... Why and Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely...gene delivery, but have limited packaging capacity making them difficult to use with CRISPR as the cargo..... 10. Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your...

...Check out this post for a refresher on AAV titers Making contact with a cell Although an individual AAV ... this type of vector to such a high titer when looking for effective gene expression. How do you achieve... Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production.     References...

...“reading”) of DNA, have changed our collective thinking about the feasible size and scope of projects ...library prep. As part of our mission, we’ve been working with Addgene to develop and apply our plexWell™...read level, and because custom primers or "primer-walking" reactions are completely unnecessary for sequencing...

...hot plasmids articles. Think we missed something smokin'? You can pitch a Hot Plasmids section (antibodies...heatshock promoter) drives expression of the tumor-seeking CAR (chimeric antigen receptor), whereas a ubiquitous... variants with similar capabilities. If you’re looking for a super stable FP for your next microscopy ...

...contributed by Eric Perkins Mammalian genomic DNA is not just a 2-D linear molecule snaking through our cells...These loops not only provide a 3-D structure for packing genomic DNA into nuclei, but are also thought to...