Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 793 results for: KIN

Showing: 401 - 410 of 793 results

  1. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...around the world who would otherwise have difficulty making or getting these reagents is profound.” Frank Stegmeier...Library, a library that “enables the high-resolution tracking of more than 1 million cancer cells under drug...became passionate about virology,” he says. He was working in an HIV lab and based on that experience, soon...

  2. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...species). This feature is advantageous if you’re working with small capacity viral vectors such as AAV. ... “recovering” its original sequence. If you’re looking to disrupt gene function via indel formation, this...targets. Are you using NgAgo? Let us know how it’s working in the comments section! Read About NgAgo User'...

  3. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC... get PCR amplification. We can start similarly, taking the final 18 bases of the ORF, including the stop...

  4. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... Why and Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely...gene delivery, but have limited packaging capacity making them difficult to use with CRISPR as the cargo..... 10. Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your...

  5. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...Check out this post for a refresher on AAV titers Making contact with a cell Although an individual AAV ... this type of vector to such a high titer when looking for effective gene expression. How do you achieve... Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production.     References...

  7. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...“reading”) of DNA, have changed our collective thinking about the feasible size and scope of projects ...library prep. As part of our mission, we’ve been working with Addgene to develop and apply our plexWell™...read level, and because custom primers or "primer-walking" reactions are completely unnecessary for sequencing...

  8. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ... apart sources of information they don’t like, looking for reasons to dismiss them. There’s evidence to...Zealand’s multiple Covid-19 lockdowns, I held mask-making workshops in my community. I brought along printed... to better meet the needs of the people we are talking to. Emma Harcourt is a PhD student in the Department...

  9. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...hot plasmids articles. Think we missed something smokin'? You can pitch a Hot Plasmids section (antibodies...heatshock promoter) drives expression of the tumor-seeking CAR (chimeric antigen receptor), whereas a ubiquitous... variants with similar capabilities. If you’re looking for a super stable FP for your next microscopy ...

  10. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...biological motion (whether endocytosis, vesicle trafficking, or muscle contractions) are produced by orchestrated...the ciliate. It’s thought that they function as a kind of armor to protect their host from predation (Petroni...
Showing: 401 - 410 of 793 results