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We narrowed to 644 results for: Uty

Showing: 401 - 410 of 644 results
  1. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    .... An internal control may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or ...many variables. However daunting designing and executing these steps may be, proper controls are a basic...
  2. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... Used since the mid-90s, this technique has revolutionised the way we apprehend molecular complexes and...wavelength, to the concentration of fluorophore in solution) are two additional parameters that can affect...
  3. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...supported/readable. Is the author or their host institution expected to provide data in formats exported ...resources can offload the burden of data storage and distribution. I would argue that the journals themselves ...
  4. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...biotinylating your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules...biotinylation reagents to help with the specific elution of biotinylated proteins. For help determining ...
  5. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...interview and sell a vision for your contribution to a Department's/Institution's success is the task. Do your...
  6. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ... genes with potentially harmful mutations in therapeutic settings. Silencing of a target gene is achieved...the backbone, shRNAs are most often expressed constitutively, either in lentiviral or retroviral backbones...
  7. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ... is a nearly ubiquitous tool, but proceed with caution! Loading controls only address one type of error...by sample concentration as well as by antibody dilution — both factors we’ve covered in our previous blog...
  8. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For...K.D. et al. A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters...
  9. Hot Plasmids: February 2026

    Type
    Blog Post
    Published
    Feb. 17, 2026, 2:15 p.m.
    ... applications like long-term imaging or super-resolution techniques. The labs of Pingyong Xu and Zhifei...specific guides. Specifically, the design process utilizes Rule Set 3, a prediction model developed by the...
  10. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist can...What happens next?”  Describe how you pushed to a solution or overcame obstacles. Take time to consider the...
Showing: 401 - 410 of 644 results