We narrowed to 904 results for: CAN

...fluorescently labeled dCas9 to a given genomic locus, you can track the localization and movement of this locus...three hairpins generates white light. All 6 gRNAs can be expressed in a single vector, pCRISPRainbow-DONOR1...chromosome capture suffer from low resolution and can’t be used in vivo. Some researchers have used fluorescently...localization of 6 chromosome-specific loci in the U20S cancer cell line./ Each color represents a gRNA targeting... do so in vivo. Catalytically dead Cas9 (dCas9) cannot induce gene editing, but it retains its gRNA-directed...

...and specify a large intervening deletion. Not only can this method precisely program large genomic deletions...sites. The lab further demonstrated that PRIME-del can be “multiplexed” using pooled paired pegRNAs.  Read...

... at [email protected]! Fun fact! Did you know you can search for plasmids from a publication by pasting...

...out, especially since videos on synthetic biology can be difficult to find. I hope that many people will...

...our “Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies...

...with a specificity far greater than the one that can be achieved using pharmacological or electrophysiological...on the other side, are too long for rAAVs, which can only package sequences shorter than 5 kb (Hirsch ...opsin expression in specific neuronal cells? This can be achieved using Cre-dependent viruses, such as ...subsequently photo-stimulated through fiberoptic cannulas directly implanted in the injection site and connected...

...editing at frequencies comparable to SpCas9. SpRY can also edit NYN PAMs, but with less efficiency and ...will have a PAM located next to it. The SpRY Cas9 can also work with NYN PAMs; it just isn’t always ideal...compatibility. This alternative PAM requirement adds a significant amount of flexibility compared to a strict single-compatibility...increases the flexibility of gRNA placement significantly over SpCas9 and more so than Cas9s with an NG...

...each target has a unique species or isotype that can be probed with a distinctly conjugated secondary ...antibody. Consider too that some secondary antibodies can cross react with other species. For example, some...

...News, and is a member of the SITN podcast team. You can follow Amy on twitter @aigilson.   Special thanks...

...uses genetically encoded fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Special...

...our “Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies... order well in advance whenever possible, as we cannot expedite the application process.  More information...

...answer any questions, and get feedback on how we can be more helpful to our community.  You'll also find...

...our new curated Microbiology Collection page, you can browse for plasmids by species and find our guides...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be...

...instead of having to work with a whole organism and can help scientists produce vital tools in the lab such...

... so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and...Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors in Genome Editing Resources...

...lines.   dCas9-VP64 Description CRISPR activation can occur through fusing dCas9 with VP64, a strong transcriptional...sequences, causing targeted gene regulation. This can be used to activate transcription during either initiation...themselves. SunTag, SAM, and VPR have all shown significant improvements upon the initial dCas9-VP64 method...Performance Generally, VPR is found to have significantly higher activation levels than the initial dCas9...

..., this method is fast! These editing frequencies can be achieved with a 30-minute incubation, even with...it quickly saw wide interest, its dimeric nature can interfere with some applications. Several labs have...

...different fluorescent proteins. Visualizing fluorescence can be challenging in plants because of the auto-fluorescence...prejudice about it. They just start and go ahead. I can see science speeding up. Addgene: Are there other... be equally high as for those who go to work in cancer biology.  References: A Golden Gate Modular Cloning...

...fashion. Addgenies swapping out printer cartridges can place the spent cartridge in the large cardboard box ...requires a significant energy expenditure here at Addgene. These are critical systems that cannot be removed...and recycling in the event the object in question cannot be washed or reused/repurposed in some fashion....

...consistently contribute to our collection so that we can offer the scientific community the latest and greatest...