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We narrowed to 898 results for: CAN

Showing: 801 - 820 of 898 results

  1. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...each target has a unique species or isotype that can be probed with a distinctly conjugated secondary ...antibody. Consider too that some secondary antibodies can cross react with other species. For example, some...

  4. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...our “Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies... order well in advance whenever possible, as we cannot expedite the application process.  More information...

  5. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...answer any questions, and get feedback on how we can be more helpful to our community.  You'll also find...

  6. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...reduce the undesired C→G or C→A conversions that can happen with BE3s. These byproducts likely resulted...even at sites previously difficult to target. ABE8s can achieve 98-99% target modification in primary T cells...other editing strategies are there? CBEs and ABEs can induce 4 out of 12 possible base-to-base changes....2020). There are also RNA base editors, which you can learn more about in this blog post: CRISPR 101: RNA...sequence preferences, evoAPOBEC1-BE4max, which is significantly more effective at editing cytosines preceded...mixed population of edits, ABEs do not display significant A to non-G conversion at target loci. The removal...the development of near-PAMless Cas9 variants significantly expands the library of accessible genomic targets...

  7. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...our new curated Microbiology Collection page, you can browse for plasmids by species and find our guides...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be...

  9. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and...Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors in Genome Editing Resources...

  10. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...lines.   dCas9-VP64 Description CRISPR activation can occur through fusing dCas9 with VP64, a strong transcriptional...sequences, causing targeted gene regulation. This can be used to activate transcription during either initiation...themselves. SunTag, SAM, and VPR have all shown significant improvements upon the initial dCas9-VP64 method...Performance Generally, VPR is found to have significantly higher activation levels than the initial dCas9...

  11. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ..., this method is fast! These editing frequencies can be achieved with a 30-minute incubation, even with...it quickly saw wide interest, its dimeric nature can interfere with some applications. Several labs have...

  12. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...different fluorescent proteins. Visualizing fluorescence can be challenging in plants because of the auto-fluorescence...prejudice about it. They just start and go ahead. I can see science speeding up. Addgene: Are there other... be equally high as for those who go to work in cancer biology.  References: A Golden Gate Modular Cloning...

  13. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...fashion. Addgenies swapping out printer cartridges can place the spent cartridge in the large cardboard box ...requires a significant energy expenditure here at Addgene. These are critical systems that cannot be removed...and recycling in the event the object in question cannot be washed or reused/repurposed in some fashion....

  15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...of transposons. Insertion of the mini-transposon can occur in two different orientations, but is strongly...encodes the mini-transposon. pEffector and pDonor can also be combined in single-plasmid INTEGRATE (pSPIN...was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical...guest blogger Leo Vo! Leo Vo is currently a PhD candidate in the Sternberg Lab at Columbia University Medical...

  16. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...Endotoxin Endotoxin contamination in vector preparations can alter the immunogenic properties of the final product... after negative staining. Empty vector particles can be identified after negative staining and appear ...

  17. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...HDR). Cpf1 also expands the number of sites that can be targeted by CRISPR to AT-rich regions or AT-rich... so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and...activity in mammalian cells. From 16 diverse Cpf1 candidates, Zetsche et al. found two that display robust... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...

  18. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...to build relationships with their colleagues. It can be easy to get caught up in the minutia of the workday... that minutia gets much more manageable when you can enjoy the people you spend 40+ hours with each week...culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the question...is certainly not the truth (partially because I can’t whistle). No, we’re not all BFFs. Yes, sometimes...

  19. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... of the laser sheet and using multi-view imaging can provide enhanced resolution (8,9).  Thus, LSFM is...adeno-associated virus)-based fluorescent labeling methods that can be used with 3DISCO to image non-transgenic animals...to photobleaching.  Furthermore, point-by-point scanning along the entire specimen makes imaging too slow...photo-bleaching or photo-damage than in conventional laser scanning microscopy . Since image acquisition is performed... milliseconds to acquire one image, thereby significantly reducing the time it takes to image through ...Similar to light microscopy, there have been significant advances in tissue clearing techniques during...

  20. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted viral vector reports...
Showing: 801 - 820 of 898 results