Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 930 results for: Gene

Showing: 801 - 820 of 930 results

  1. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ... develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene Blog Which...Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...

  2. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ... [the] academic workforce is pyramidal/feudal generating too many trainees per PI” “Lack of awareness ...Dinman, Chair of the Cell Biology and Molecular Genetics Department at University of Maryland College Park...biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career...Advice on Networking at Scientific Meetings from Addgene Executive Director Joanne Kamens Learn how to be...

  3. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...and goal of the experiment. Now, onto the tips! General principles pegRNA design If you’re just starting...programs to help you design pegRNAs. Here on the Addgene blog, we featured a guest blog by the developer...s41586-024-07259-6 Additional Resources on the Addgene blog Prime Editing: Adding Precision and Flexibility... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...

  4. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...Intro to Antibodies post Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ... mx+b?). Figure 5: A standard curve is generated by plotting OD against known concentrations of...s00018-019-03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages...

  5. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...recognize multiple isotypes from a species, like a general anti-mouse antibody. Others recognize specific ...better choice for a mouse monoclonal IgG2a than a general anti-mouse IgG. In a broadly specific secondary...narrowly specific secondary antibodies rather than generally reactive ones. And make sure to check for any ....1371/journal.pone.0038313 More resources from Addgene The Antibody Guide Antibodies 101: Isotypes Antibodies...

  6. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...study binary protein interactions. However, FRET generally only tells you if two proteins interact and cannot...with your research? Plasmids are available from Addgene! References Schopp, I.M., Ramirez, C.C., Debeljak...Central PMCID: 5053326. Additional Resources on the Addgene Blog Learn about techniques for visualizing protein...-DNA Interactions with CUT&RUN Resources at Addgene.org Find plasmids from the Bethune Lab Find Plasmids...

  7. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Heckler. Additional Resources on the Addgene Blog Get Addgene's Take Aways from the FoR 2015 Symposium...Gelbart, PhD - Director of Programs at Personal Genetics Education Project Lisa Coleman, PhD - Chief Diversity...2. Taylor, Justin Q., et al. “Avoiding a lost generation of scientists.” eLife 5 (2016): e17393. Pubmed...

  8. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...conferences Resources on the Addgene website Become of a member of the Addgene depositor community Learn... science communication.       Resources on the Addgene blog Read other posts on scientific communication...Learn about careers at Addgene ...

  9. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...and Success in Management Resources at Addgene.org Addgene's Management Team ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...Business School Blog Additional Resources on the Addgene Blog Management for Scientists Series What Makes...

  10. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...obscures the flexible nature of all proteins in general, and the amazing conformational flexibility that...classroom or trying to explain your science to the general public, visit the Center for BioMolecular Modeling...Molecular Designs.     Additional Resources on the Addgene Blog Learn All about CRISPR Use CRISPR for Genome...User by a First Time CRISPR User Resources on Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History...

  11. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ... as Customer Support and Operations Manager at Addgene. In this role, I balance my daily tasks, meet cross-team...former Customer Support and Operations Manager at Addgene and is interested in nonprofit management and international...development.       Additional Resources on the Addgene Blog Get tips on writing a good cover letter Resume... careers Learn about mentoring Resources on Addgene.org Check out our careers page Watch our career ...

  12. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...back-bone specific primers. Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which...them onto the gel. The figure above summarizes generalized expected results for the three primers previously...Overview of Colony PCR Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC ...about Gibson Assembly Additional Resources on Addgene.org Read about Choosing a Molecular Cloning Technique...

  13. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...little as an hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection system ...Cas12a however recognizes a different PAM site and generates 5’ and 3’ staggered ends after dsDNA breaks. The...j.cell.2015.09.038 Additional resources on the Addgene Blog Learn about using toehold switches to detect...multiplex genome editing abilities Resources on Addgene.org Checkout our CRISPR guide page Browse the CRISPR...

  14. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our career videos ...bioengineered scaffolds with patient stem cells to generate compatible functional livers.” Why are you the...outdoor photographer.  Additional resources on the Addgene blog Find tips for presenting a science talk Check...

  15. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...biology you will study. (You can also check out Addgene’s blog, eBooks, and other educational resources ...everything! The bare minimum should be taking notes on general protocol steps and other tips and tricks your mentor...my dissections go smoother next time?” or more generally “How am I doing? Can you tell me what’s going ...personnel! Happy training! Resources Resources on the Addgene blog Trading Spaces – Visiting Scientist Edition...

  16. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...Career Options Resources on Addgene.org Learn more About Careers at Addgene ...incorporated example documents. This part has generated a lot of positive feedback. Many of these documents...gardener, and cook.  Additional Resources on the Addgene Blog Academic v. Non-Academic Career Decisions...

  17. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...skills guide Resources on Addgene.org Learn more about Careers at Addgene Watch out career videos   ...better slides that more effectively tell a story? Generally, the answer to the second question is yes. As ...outdoor photographer.  Additional Resources on the Addgene Blog Find Tips for getting a faculty position ...

  18. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...Here at Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. ...and tweeting it with the tags #DeckTheLab and @Addgene. We’d love to see how you’re decorating your lab...lucky winner? Happy holidays from all of us at Addgene! We look forward to seeing how you #DeckTheLab!...

  19. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ... and do not necessarily represent the views of Addgene. Boston, MA, USA 42.3601°N 71.0589°W – Alina Chan...scientists, the legacy of each government and generation is in the veracity of its work. Alina Chan is...Aurora, CO where she studies the development and regeneration of mammalian taste buds. Erin is also the President... and @SciCATSyvr. Additional Resources on the Addgene Blog Read Our Previow Post on the March for Science...

  20. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...molecule (see figure to the right). The excitation generated by these two-photons occurs only at a chosen focal...remain. The use of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create...PubMed PMID: 18431356. Additional Resources on the Addgene Blog Get Tips on Choosing Your Fluorescent Protein...Monitor Protein-Protein Interactions Resources on Addgene.org Check out Our Fluorescent Protein Guide Pages...
Showing: 801 - 820 of 930 results