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We narrowed to 939 results for: Gene

Showing: 801 - 820 of 939 results
  1. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...protein. The pCri system, recently deposited to Addgene by F. Xavier Gomis-Rüth, aims to take the guesswork... either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The ...visualization of the data from RSCB PDB Resources on the Addgene Blog Plasmids 101: E. coli strains for protein...expression Plasmids 101: Protein tags Resources on Addgene.org Find Additional Cloning Kits ...
  2. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased towards... likely due to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and...reviewing this blog post. Additional Resources on the Addgene Blog Learn about Homology Directed Repair 3 Tips...Efficiency How to Design Your gRNA Resources on Addgene.org Check out CRISPR Topic Page Find CRISPR Nickase...
  3. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...When I started writing for the Addgene blog, I was focused on writing about new scientific techniques...first program I used to create graphics for the Addgene blog. However, it has a limited number of drawing...body, cellular biology, medical specialities, and general items. Individual images as well as collections...comments section!  Additional resources on the Addgene blog Read our Early Career Researcher Toolbox ...
  4. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ...scientists here Resources on Addgene.org Learn more about careers at Addgene ...posts in this series! Additional Resources on the Addgene Blog Learn more about alternative science careers...creative science education tools? Check out this Addgene podcast with educator and science rap mastermind...
  5. Are Hybrid Guide RNAs Right for Your CRISPR Application?

    Type
    Blog Post
    Published
    Jan. 27, 2026, 2:14 p.m.
    ...We at Addgene heard about RNA-DNA hybrid guides at the annual American Society of Human Genetics meeting...10.1038/nchembio.2559 Additional resources on the Addgene blog Build Your CRISPR Vocabulary A Needle in ...Adenine Base Editors Additional resources on addgene.org Read the CRISPR guide Download the CRISPR 101...
  6. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...Scientists Series Resources at Addgene.org Learn About Careers at Addgene ...requirements for hiring more common Likelier to hire on general talent and not specific skills <- Can move between...need to get there. Additional Resources on the Addgene Blog Learn about Possible Science Careers How ...
  7. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...communications efforts.  Scicomm definitions (as used by Addgene)    Science communication, or science writing, ...of knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers...AP biology classes Creating a podcast about genetics using dog breeds as an example adults 45-55 yrs... you next time!  Additional resources on the Addgene blog 10 Ways to Share Your Science Science Communication...
  8. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...not permeate through your tissue samples as homogeneously as a single antibody. Using a secondary antibody...10.1177/38.7.2355173  Additional resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence...
  9. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ... a Good Cover Letter Resources on Addgene.org Careers at Addgene Global Plasmid Distribution ...The contents of this post are intended to convey general information only and not to provide legal advice...you think about your career (and your future in general), keep in mind that you may have years to work ...www.casseuslaw.com.    Additional Resources on the Addgene Blog Transferable Skills Guide 6 Steps to Submitting...
  10. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ... develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene Blog Which...Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...
  11. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ... [the] academic workforce is pyramidal/feudal generating too many trainees per PI” “Lack of awareness ...Dinman, Chair of the Cell Biology and Molecular Genetics Department at University of Maryland College Park...biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career...Advice on Networking at Scientific Meetings from Addgene Executive Director Joanne Kamens Learn how to be...
  12. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...study binary protein interactions. However, FRET generally only tells you if two proteins interact and cannot...with your research? Plasmids are available from Addgene! References Schopp, I.M., Ramirez, C.C., Debeljak...Central PMCID: 5053326. Additional Resources on the Addgene Blog Learn about techniques for visualizing protein...-DNA Interactions with CUT&RUN Resources at Addgene.org Find plasmids from the Bethune Lab Find Plasmids...
  13. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...Intro to Antibodies post Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ... mx+b?). Figure 5: A standard curve is generated by plotting OD against known concentrations of...s00018-019-03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages...
  14. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...and goal of the experiment. Now, onto the tips! General principles pegRNA design If you’re just starting...programs to help you design pegRNAs. Here on the Addgene blog, we featured a guest blog by the developer...s41586-024-07259-6 Additional Resources on the Addgene blog Prime Editing: Adding Precision and Flexibility... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...
  15. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...recognize multiple isotypes from a species, like a general anti-mouse antibody. Others recognize specific ...better choice for a mouse monoclonal IgG2a than a general anti-mouse IgG. In a broadly specific secondary...narrowly specific secondary antibodies rather than generally reactive ones. And make sure to check for any ....1371/journal.pone.0038313 More resources from Addgene The Antibody Guide Antibodies 101: Isotypes Antibodies...
  16. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Heckler. Additional Resources on the Addgene Blog Get Addgene's Take Aways from the FoR 2015 Symposium...Gelbart, PhD - Director of Programs at Personal Genetics Education Project Lisa Coleman, PhD - Chief Diversity...2. Taylor, Justin Q., et al. “Avoiding a lost generation of scientists.” eLife 5 (2016): e17393. Pubmed...
  17. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...and Success in Management Resources at Addgene.org Addgene's Management Team ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...Business School Blog Additional Resources on the Addgene Blog Management for Scientists Series What Makes...
  18. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...conferences Resources on the Addgene website Become of a member of the Addgene depositor community Learn... science communication.       Resources on the Addgene blog Read other posts on scientific communication...Learn about careers at Addgene ...
  19. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...obscures the flexible nature of all proteins in general, and the amazing conformational flexibility that...classroom or trying to explain your science to the general public, visit the Center for BioMolecular Modeling...Molecular Designs.     Additional Resources on the Addgene Blog Learn All about CRISPR Use CRISPR for Genome...User by a First Time CRISPR User Resources on Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History...
  20. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ... as Customer Support and Operations Manager at Addgene. In this role, I balance my daily tasks, meet cross-team...former Customer Support and Operations Manager at Addgene and is interested in nonprofit management and international...development.       Additional Resources on the Addgene Blog Get tips on writing a good cover letter Resume... careers Learn about mentoring Resources on Addgene.org Check out our careers page Watch our career ...
Showing: 801 - 820 of 939 results