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TypeBlog PostPublishedSept. 12, 2019, 1 p.m....can also request the specific serotypes you need instead of receiving a kit with all the serotypes, since...
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New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog PostPublishedDec. 20, 2018, 1:15 p.m.... consensus to the next meeting of experts, and instead convene the necessary groups for the necessary ... -
Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....journals as those that did make deposits to Addgene. Insomuch as different journals represent different research... -
Science Career Options
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m....available and then decide on a course of action. Instead of rushing in, you take steps to make it more likely... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....topics and interests. Offer help when you can, instead of just asking for favors. Don’t focus on networking... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....human cells via delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019.... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m.... the end product would likely be poor quality. Instead, we pull together teams of individuals who enjoy... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....regulate transcription. Red flame plasmid pDawn contains the blue-light sensing protein YFI. When light... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....AAVrg while other pathways such as those in the brainstem were not. Others have noted a lack of transduction... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m.... using your single color controls (Figure 1A), instead of using your actual samples. They may also be ... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....careers Plasmids CRISPR Viral vectors Fluorescent proteins ... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog PostPublishedJan. 30, 2020, 2:15 p.m....which often focuses more attention on the results instead of the methods. Unreported flexibility and questionable... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....They all found the support of the group to be intrinsic to their success. Their stories made it clear ... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....up in both science journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....oligonucleotide (ssODN) template supplied by Ma et al., but instead from the mother’s wild type allele (Figure 1). ... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m.... with very little text on the slides and notes instead of written speeches. When you’re doing all that... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m....addition to the 20 bp guide sequence, the crRNA also contains a ~20 bp repeat region on its 3’ end. This repeat... -
Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m.... Eric J. Perkins...