We narrowed to 813 results for: tes
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TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....envelope plasmid you use as the choice of envelope dictates the tropism of the virus. VSV-G is very common...
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Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....amplification. Transformed E. coli are allowed to grow on plates or in liquid culture to amplify the library, followed... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....was most productive in short bursts (even twenty minutes was enough to make some progress). For most researchers... -
5 Reasons to Use Reddit for Science Communication
TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m....too many to describe here, but I have over 2,000 notes which Evernote even allows me to access on my phone... -
CRISPR Activation: A Practical Guide
TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m....you want to activate, you should also be ready to test three or four guides directed towards that gene ... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....vesicular stomatitis virus G protein (VSV-G), which facilitates viral entry into the host cell. Transfecting... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....doi.org/10.1038/s41565-019-0375-4 Lowder LG, Zhang D, Baltes NJ, Paul JW III, Tang X, Zheng X, Voytas DF, Hsieh... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 2:15 p.m....transduce neurons for retrograde labeling, as well as astrocytes in the CNS (Han et al., 2023), and has also been... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m.... the strong yeast promoter pTEF). As ethanol accumulates, it binds to the repressor, enabling it to bind... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m.... clustering and discovery algorithms find new candidates for CRISPR proteins. A type VII is already on... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....descriptions first before entering the data. This step associates an Addgene ID with your plasmids, and adding ...