We narrowed to 904 results for: CAN

...working integrase, IDLVs can still deliver their genetic payload to the cell but cannot integrate it into the...tools for manipulating cells and organisms. They can be used for a variety of research purposes, like ...cells, among many other uses. Lentiviral vectors can be produced easily, without the need for time-consuming...is then part of the cellular DNA… forever!  This can be an advantage for researchers looking to generate... for integration, two types of circular episomes can be generated through either homologous recombination...expression of Cas9 is no longer necessary. However, Cas9 can be toxic if continuously expressed (Fu et al., 2013...3. Stable expression in non-dividing cells IDLVs can also be useful for transducing non-dividing cells...

...or A much further from the ‘NGG’ PAM cannot be editable? They can, but they require other base editor ...about CRISPR, one question probably comes to mind: can CRISPR be applied to fix the defective base precisely...CRISPR-mediated base editing (BE), these types of repairs can now be done more efficiently than before. A base ...guanine (G)? You may wonder if a CRISPR-base editor can perform this change. However, there are no naturally...successful evolution of an adenosine deaminase that can act on DNA for adenine base editing. They first created...pKABE6.3, pKABE7.9, and pKABE7.10) so that the user can optimize base editing efficiency based on the location...to the protospacer adjacent motif (PAM). ABE7.10 can edit target 'A' if it lies in a small genomic window...

...master mix of enzymes can be purchased from a company (e.g. NEB or SGI-DNA), or can be mixed yourself (e.g...easy assembly of multiple linear DNA fragments, but can also be used in basic cloning of an insert into your...great part about this mix of enzymes is that they can all work at the same temperature, so the entire reaction...Miller Lab Protocol). The Gibson assembly process can be used to assemble up to 6 fragments in one step... required homology between neighboring fragments can be created via PCR amplification with primers that...fragments. However, by using Gibson assembly, you can insert both the gene of interest and the tag sequences... Excluding the time it takes to get primers, you can have your construct made in 5 days. One drawback...

...cloning (discussed below) can run on the order of $20-30 per reaction but can be used to quickly generate...fragments in a prescribed order. These fragments can be pooled in one tube and ligated in as little as...needed for this method is relatively inexpensive and can be obtained for about $70, resulting in a price of...dollars per reaction. By comparison, Gibson assembly can cost over $15.00 per reaction if you purchase a pre-made...remember that commercially available cloning kits can often make the process much more streamlined, because...homology based methods, like Gibson or SLIC, since one can pool the components and define their final order ...

...blue. Students can create synthetic riboprobes off of linearized DNA templates which they can easily generate...differ. For example, Girardia sp. and Dugesia dorotocephalacan can be fed dsRNA-expressing E. coli that are...version of L440. This vector is versatile in that it can be used for both in situ hybridization and dsRNA-...bidirectional transcription to create dsRNA. Students can produce DNA templates via PCR off of the same plasmid...comparison. Performing WISH using these plasmids can result in  the labeling specific cell types or entire...number of genes that other classrooms and researchers can easily investigate in planarians. References 1. ...Kimberly C. Tu, Alejandro Sánchez Alvarado, The American Biology Teacher, Vol. 79 No. 3, March 2017; (pp...

...researcher can cause them to interact by stimulation with blue light.  Similarly, light can also be used...further probe cellular function. The CRY2clust Plasmid can be found here. Park et al. Nat Commun. 2017. PubMed...“addicted” E. coli strain expressing this operon can serve as a living biosensor for measuring caffeine...pDCAF3 operon, the growth of this knockout strain can be rescued by the xanthine produced via complete ...organization. The design use to detect PKA activity can be generalized to measure phosphatase activity, second...proteins have improved photophysical properties and can be used in quenching, FRET (Förster resonance energy...

...split-Cre systems which can take up to 24 hours to cut, Opto-Cre-Vvd is fast and can cut in as little as ...utilizing recombinases to manipulate genomes, scientists can control expression of their genes of interest. While...expression tools in and of themselves, their activity can be fine-tuned and precisely controlled through the...sensitivity to ambient light, and versatile as it can be activated by both low and high-intensity blue ...single active site. The RuvC active site in Casɸ can both process crRNA and carry out the crRNA-guided...human and plant cells. Casɸ from Biggiephage can eliminate competing mobile genetic elements. Image...

...drop-down and contact Addgene. We can only accept plasmids that can be used under BSL1 conditions.  ...deposit spreadsheet?  Our plasmid deposit process can be broken down into four simple steps: Send plasmid...most involvement with Step 1, and, while data entry can be a chore, we’ve made it easy to send us your plasmid...others need to be filled with short-answer text. You can also directly paste sequence data into the appropriate...rabbit), synthetic, or other. If other, the species can be indicated in the “Species Other” column. Relevant...QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format. Distribution...replication, choose the Pir1 strain. If your plasmid cannot be propagated in any of these standard strains,...

...of accumulated wisdom so that you, the customer, can get the most out of your email or phone call. Though...saying “Hey look! This stuff works!” These papers can be a particularly good resource if you are considering...Hi! We ordered something from you and it’s wrong. Can you help us fix it? Please address as soon as possible...begin my responses asking for more specifics. We can help you faster if we get as much information as ...least the plasmid name in your initial query. This can reduce the number of messages in our thread or remove...but a picture of a gel or a sequencing trace file can go a long way in helping us understand the nature... be courteous. We understand how exasperating it can be to lose time and money on a potentially faulty...

...protocol can save weeks of trial-and-error. A recommendation for the correct statistical test can save a...Spain and works in the field of genetics and cancer. You can follow her on Twitter at @mahesa2504       ...community where both brief and long-term collaborations can be easily started and easily credited. And nothing...scientific research. It’s completely free, of course. You can find more information about us and what we do in ... based in Spain and works on climate change. You can follow him on Twitter at @IgnacioAmigoH     María...while most of the world was locked down, a South African company wanted to test if a type of antibodies ...