Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 892 results for: CAN

Showing: 411 - 420 of 892 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...drop-down and contact Addgene. We can only accept plasmids that can be used under BSL1 conditions.  ...deposit spreadsheet?  Our plasmid deposit process can be broken down into four simple steps: Send plasmid...most involvement with Step 1, and, while data entry can be a chore, we’ve made it easy to send us your plasmid...others need to be filled with short-answer text. You can also directly paste sequence data into the appropriate...rabbit), synthetic, or other. If other, the species can be indicated in the “Species Other” column. Relevant...QUEEN-generated GenBank files (Mori and Yachie, 2021) but can accept sequence files in any format. Distribution...replication, choose the Pir1 strain. If your plasmid cannot be propagated in any of these standard strains,...

  2. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...split-Cre systems which can take up to 24 hours to cut, Opto-Cre-Vvd is fast and can cut in as little as ...utilizing recombinases to manipulate genomes, scientists can control expression of their genes of interest. While...expression tools in and of themselves, their activity can be fine-tuned and precisely controlled through the...sensitivity to ambient light, and versatile as it can be activated by both low and high-intensity blue ...single active site. The RuvC active site in Casɸ can both process crRNA and carry out the crRNA-guided...human and plant cells. Casɸ from Biggiephage can eliminate competing mobile genetic elements. Image...

  3. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...of accumulated wisdom so that you, the customer, can get the most out of your email or phone call. Though...saying “Hey look! This stuff works!” These papers can be a particularly good resource if you are considering...Hi! We ordered something from you and it’s wrong. Can you help us fix it? Please address as soon as possible...begin my responses asking for more specifics. We can help you faster if we get as much information as ...least the plasmid name in your initial query. This can reduce the number of messages in our thread or remove...but a picture of a gel or a sequencing trace file can go a long way in helping us understand the nature... be courteous. We understand how exasperating it can be to lose time and money on a potentially faulty...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ... been motivated to do anything and everything we can to help find a cure -- and quickly. Normally drug... approach is to discover key human proteins that can be targeted (hopefully by existing therapeutics that...these experiments, there's so much to do that we can use the whole science community's help. You could... but one are in a pLVX vector background, so you can package them into lentiviral vectors if you need ...FedEx guy has been the best: Thanks, Todd! As you can imagine, it’s also been quite the administrative [email protected] with your order number and so it can be prioritized. ...Arabia -- as well as closer to home in the US and Canada. In fact, we received so many requests (175 and...

  5. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...protocol can save weeks of trial-and-error. A recommendation for the correct statistical test can save a...Spain and works in the field of genetics and cancer. You can follow her on Twitter at @mahesa2504       ...community where both brief and long-term collaborations can be easily started and easily credited. And nothing...scientific research. It’s completely free, of course. You can find more information about us and what we do in ... based in Spain and works on climate change. You can follow him on Twitter at @IgnacioAmigoH     María...while most of the world was locked down, a South African company wanted to test if a type of antibodies ...

  6. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...differentiation To understand how each human TF can contribute to differentiation, the Zhang lab transduced...Similar to the Human TFome Library, the MORF library can be used with other selection and screening mechanisms...library webpage. Disease models Good disease models can often be difficult to generate, in part because of... dysregulation. A targeted TF screening approach can not only lead to a better understanding of disease...simulate a disease in a model system. TF screens can be designed to identify custom TF programs to acutely...and GFP fluorescent controls. Alternatively, you can order individual plasmids from the library to further...Individual cellular fates were then matched with candidate TFs responsible for that differentiation.   ...

  7. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... light exposure can also lead to cellular phototoxicity, and experimental success can be affected by both...biologists over the last two decades, but their use can be limited by a few caveats. Since fluorescent proteins...and showed that these three luminescent proteins can be used together in cells. As Nano-lanterns are not...fluorescence, so higher concentrations of the compound can be added to the media. Cellular esterase then converts...optogenetic tools, where light used for excitation can cause unintended activation of the optogenetic system...proteins (FP) require external light activation, you can’t use fluorescence to monitor processes directly affected... physiology in your system. Optical sectioning cannot be conducted with luminescence imaging since there...

  8. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...Addgenie expertise we can. We even have a Slack channel just for asking people if they can write or review ... is our Help Center, help.addgene.org, where you can easily search articles answering all of our frequently... our depositors and customers are asking. So how can you get involved? Keep asking your burning questions...and tell us what you want to know more about! You can also suggest blog topics or even reach out about ...support and advance the scientific community, and we can only do that with your help.     Additional Resources...

  9. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...genome. Thus the gRNAs responsible for a phenotype can be identified by next-generation sequencing of the...readout. This retroviral mouse CRISPR pooled library can be used for screening of cells that are refractory...lipoproteins through the circulatory systems, but can also lead to the formation of atherosclerotic plaques...transmit electrical information. Such voltage imaging can be performed in intact animals and offers many advantages...found several voltage biosensors and conditions that can be used to monitor real time voltage changes in worms...-mOrange and MacQ-mCitrine. All of these sensors can detect action potentials in worms with fluorescence...libraries was not possible. Primary murine T cells cannot be transduced by lentivirus and have been difficult...

  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...protein interacts with that one promoter can tell you, it can be easy to get lost in the details. The ...like stem cell biology where questions of ethics can have profound impacts on the development of applications...and appreciate the importance of ethics, the topic can often take the back burner at scientific conferences...international borders. While questions of ethics can be daunting because they have no clear cut answers...stem cell community with their research, I think I can speak for Addgene when I say that I'm glad we're ... important questions that the scientific method cannot address. Listen to Our Podcast Interview with Stem...
Showing: 411 - 420 of 892 results