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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 793 results for: KIN

Showing: 411 - 420 of 793 results

  1. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...Cas9, and the tracrRNA which acts as a scaffold linking the crRNA to Cas9 and facilitates processing of...different components of CRISPR, It's time to start thinking about how you can used them in your experiments...experiments. Use the following flow chart to get you thinking about the types of experiments you're planning on...

  2. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...idea to take some time to confirm that you are working with the correct construct, and verify that the...Consult the manufacturer’s manual for the optimal working conditions for each enzyme. Watch out for methylation...run control uncut DNA to ensure your enzymes are working. When uncut plasmid DNA is isolated and run on ...

  3. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...think. So, if you’ve ever wondered what goes into ‘making virus’ or what gag and pol stand for, we demystify...recombinant adenovirus is missing the endogenous E1 gene, making it replication deficient. This is typically supplied...discussed are compiled for reference below. Happy virus making!  References and Resources Kulanayake, S., Tikoo...

  4. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...can begin to understand how the brain works. It’s kind of like “the head bone’s connected to the neck bone... to the rest of the body. Therefore, by simply looking at how neurons are physically connected we can ...efficiently deliver genetic information or tools, making them useful tracers, but finding a virus that works...

  5. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...question that didn’t get answered live - but he kindly took some time to address all your unanswered questions...astrocyte specificity and would like to get it working in microglia as well. A:  We are not aware of any... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ...

  6. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ..., you may be curious about all of the different kinds of antibodies that are available. One common type...References Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B,...TA, Humphries MJ (2016) Ligand-induced Epitope Masking. Journal of Biological Chemistry 291:20993–21007...

  7. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...— by directly typing terms in a search bar, by ticking some boxes, by selecting a suggestion — how should...resources. Filters We identified the most useful kinds of information per resource category to expose as...our search indices in order to support different kinds of matches: exact words, partial words, phrases,...

  8. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...These enzymes also do not require a protospacer flanking sequence, so you can target virtually any RNA ...Cas13d enzymes have a median length of ~930aa - making them 17-26% smaller than other Cas13s and a whopping...and we at Addgene appreciate our depositors for making their tools available to the broader community....

  9. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled library screening experiments...V2.0 was made available through Addgene in 2015, making this the most recently available plasmid in the... thank all of our depositors and requestors for making their reagents available and for supporting scientific...

  10. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...reference protein) under identical conditions, a ranking of the practical brightness can be made (Goedhart...repeating this with another fluorescent protein, a ranking of practical brightness can be made. This strategy...their practical brightness in a system closely mimicking the future application. In figure 2 we provide...
Showing: 411 - 420 of 793 results