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We narrowed to 644 results for: Uty

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Showing: 411 - 420 of 644 results

  1. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...demonstrated two potential strategies for CRISPR-HIV therapeutics - one using dCas9-SAM to activate HIV-1 transcription...next few years, as it would open up many new therapeutic possibilities for some of the world’s most common...

  3. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...knockout.  If knockout samples are unavailable, try substituting with knockdown approaches using siRNA or shRNA...either by modifying the blocking conditions or by substituting for a more specific secondary antibody. The ...

  4. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...them? Or maybe you just want to make some really beautiful images? Multiplex IF may be just what you need... L., Zhu, Y., Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex Immunofluorescence...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... for i-GONAD. A micropipette is used to inject solution into the oviduct wall between the ampulla and ...incision that exposes the ovaries and oviducts. After solution injection into the oviductal lumen, tweezer-type...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...modification was impossible. With the genome engineering revolution came epigenome-engineering tools - zinc finger...transduced cells. Grant Challen’s lab also created constitutive (pCMV-dCas9-D3A) and Tet-dependent (TetO-dCas9...

  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... percentage of acrylamide. To further improve resolution, use a stacking gel on top of the resolving gel...Gradient gels are another great option to enhance resolution. In this setup, gels have an increasing range...

  8. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...SV40, are always active and thus referred to as constitutive promoters. Others are only active under specific...that also express GAL4. Usage of this system revolutionized genetic studies in flies. An additional component...

  9. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...only semi-quantitative. It won’t tell you the distribution of states in a sample; instead, it will suggest...the Lu Lab at MIT. He is broadly interested in biocomputing and biological circuits.     References 1. N...

  10. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...Wenning Qin and Haoyi Wang. CRISPR/Cas9 is revolutionizing the mouse gene-targeting field. Mice have long... he is interested in using CRISPR to develop therapeutic applications that better human health.     References...
Showing: 411 - 420 of 644 results