We narrowed to 835 results for: Abo;

1. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ... & Corey, D. R. (2021). Reexamining assumptions about miRNA-guided gene silencing. Nucleic Acids Research...

2. Hot Plasmids: Summer 2024

   Type

   Blog Post

   Published

   Aug. 27, 2024, 1:15 p.m.

   ...transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration with the Vallabh/Minikel Lab, the Weissman Lab...

3. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   Published

   Oct. 11, 2016, 2:30 p.m.

   ... Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits ...

4. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ... Brush up on Your Cloning Techniques Learn More about Plasmids in Our Plasmids 101 Series Use CRISPR to...

5. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ...reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune...

6. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Sept. 24, 2020, 1:15 p.m.

   ...target should be near the TSS but you can worry less about sequence optimality because you generally have fewer...

7. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   Published

   June 27, 2023, 1:15 p.m.

   ...which tools to use, and what do you need to know about them to be successful in your experiments? In this...

8. Viral Vectors 101: AAV Variables That Matter

   Type

   Blog Post

   Published

   June 1, 2023, 1:15 p.m.

   ...starting the selection process. Factors to think about are (1) your experimental conditions and measurements...

9. Antibodies 101: Multiplex Immunofluorescence

   Type

   Blog Post

   Published

   May 23, 2023, 1:15 p.m.

   ...to label the rat brain sample shown in Figure 2A above, Andrews et al. used the following combination of...

10. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...our planet, but we still know collectively little about how they infect and influence the evolution of their...

11. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type

    Blog Post

    Published

    March 21, 2024, 1:15 p.m.

    ... A., Purkayastha, N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert, D. (2020...

12. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...annotate on this page. Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information ...

13. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ... should have a melting temperature 5-10 degrees above the primer and be at least 25 bp away from each ...

14. Technical Design of a Western Blot

    Type

    Blog Post

    Published

    Aug. 22, 2024, 1:15 p.m.

    ...If you’ve ever run a western blot, or thought about running one, you’ll know there’s a lot of choices...

15. Using AAV for Neuronal Tracing

    Type

    Blog Post

    Published

    Aug. 9, 2018, 1:04 p.m.

    ...usually severely impaired, and only few reports exist about dye molecules that are transported from one neuron...