We narrowed to 847 results for: CAL;

1. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ... types of proteins. They started with another biological workhorse, Cre recombinase, which induces recombination...

2. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   ...Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical Development 9:390–401 . https://doi.org/10.1016...

3. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...s outer membrane and allows the components to physically enter the cells so they can go to work. ...

4. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   Blog Post

   Published

   Sept. 26, 2019, 12:50 p.m.

   ...cytoplasm or pronucleus of the zygote which is both technically challenging and can negatively impact zygote ...

5. Open Resources and Plasmid Tools For Studying C. elegans

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   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...Biodesign institute developed a 3’UTR database specifically for C. elegans. This database provides detailed...

6. Popular Retroviral Vectors and Their Uses in Scientific Research

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   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...-humanized (Plasmid #44246) Expression of a catalytically inactive (dead), human codon-optimized Cas9 ...

7. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ...and Change in Undergraduate Biology Education: A Call to Action, Washington, DC. 2. President's Council...

8. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ... not schematized. Sizes of fragments are not to scale. Figure and caption from McGee et al., 2024, used...

9. Antibodies 101: Stripping and Reprobing Western Blots

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   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...reacts with antibodies, so thorough washing is critical. Assess the stripping Now it’s time to check ...

10. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...downstream of the gene to be transcribed, and typically occur directly after any 3’ regulatory elements...

11. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...,000 colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer per 1,000,000...

12. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    Published

    July 12, 2018, 1:09 p.m.

    ...analysis of bisphenol A-induced perturbation in biological processes in ER-negative HEK293 cells." PLoS ...

13. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ...:  my undergrads were different people! More specifically, they had different learning styles. One was...

14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ...Addgene. 2. Dalvai, Mathieu, et al. (2015). “A Scalable Genome-Editing Based Approach for Mapping Multiprotein...

15. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type

    Blog Post

    Published

    Sept. 23, 2014, 3:05 p.m.

    ...2,500 protocols covering a variety of bio and biomedical topics. Protocol Online Website: http://www.protocol-online.org...

16. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ... useful also. There is also a U.K. organization called Athena SWAN that is a charter for advancing women...

17. Tips for arabidopsis transformation

    Type

    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ...plants: Lots of plants produce lots of seeds. I typically use 8-10 plants per construct I transform. More...

18. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...-up Setting up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine ...

19. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...Updated Aug 13, 2020. Note: Cpf1 is also called Cas12a. In 2015, Zetsche et al. added to the CRISPR ...

20. Transferable Skills Guide: Creativity

    Type

    Blog Post

    Published

    Jan. 18, 2022, 2:15 p.m.

    ...community is supply chain shortages. When flasks, conical tubes or pipette tips are backordered for months...