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Showing: 821 - 840 of 889 results

  1. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...synthesized endogenous proteins in situ. Now FUNCAT-PLA provides both quantitative and spatio-temporal information...protein synthesis: incorporation of puromycin into peptide chains." Proceedings of the National Academy of...

  2. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... Kramer L, Campbell RE. Structure-and mechanism-guided design of single fluorescent protein-based biosensors...1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral...

  3. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...indirect ELISA is very similar to a direct ELISA, aside from the detection step. Similarly, the protein ... if that’s the option you choose. Once you’ve identified the linear portion of your standard curve, you...

  4. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ... excited about. Following scientists in fields outside your main research focus lets you keep a pulse ...window of time for publication. To help you quickly identify papers with greater influence, the average number...

  5. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...leadership and management skills. The NPA brought outside experts and many hands-on- and role-playing exercises...with other leaders in my research field should I decide to remain here.  In a time when it is so important...

  6. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...stranded break (DSB) at a genomic site specified by a guide RNA (gRNA) sequence. Users can design gRNAs to induce...SapTrap, scientists will be able to generate genome-wide libraries containing fluorescently-tagged proteins...

  7. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...strains on the blog, and also recommend this handy guide from OpenWetWare for a more extensive listing of...often ambiguous and should be viewed more as a guideline, since they are typically based on the copy number...

  8. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...plasmids tend to be high copy number. Here, we will consider the replication mechanism of plasmids under stringent...plasmid to balance the total plasmid number.  Let’s consider pSC101. This plasmid is stringently controlled...

  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...expression. Take a look at all of the options and decide what might work best for your experiments! This...Additional Resources on the Addgene Blog Quick guide to working with Drosophila Terminators and PolyA...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...orange and red fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns...bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Reports 7, 42398. PubMed PMID...

  11. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...channel for more helpful animations, useful protocol videos, and quick tips for lab success.  ...

  12. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...quest, which led us to Seqera Labs, a company that provides open source workflow orchestration software for...

  14. The Twelve Days of Antibodies

    Type
    Blog Post
    Published
    Jan. 6, 2025, 5:17 p.m.
    ...Antibody, Addgene gave to me…  An online Antibody Guide!    🎶On the fourth day of Antibody, Addgene gave...

  16. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...decision between some meaningful names…. Watch the video to find out the final four! (Note: For cases where...

  18. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured over the...

  20. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...the 2nd day of CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with...
Showing: 821 - 840 of 889 results