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  2. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ... Genome-wide Inhibition Library. DNA and cell amounts After deciding on a cell type, you will need to...

  3. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...hoping that your lab mate would have time to make comments on your manuscript. If you give her this slot,...

  4. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...Nanobodies also simplify multicolor staining experiments because they don’t require using primary antibodies...

  7. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

  8. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    .... You can get your bacteria to produce massive amounts of protein, but if it’s not folded correctly, there...

  10. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...

  12. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...

  16. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...hermaphrodites making it easy to conduct large experiments over multiple generations. Similarly to other...

  17. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...surface of the CD8+ T cells and gate on the positive events for your flow analysis. However, certain downstream...

  19. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...strains, especially mutations that make major improvements such as those that increase plasmid yield and...
Showing: 821 - 840 of 840 results