We narrowed to 854 results for: NTS;

1. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ...the best advice is to try a couple of promising variants to check how they perform under your experimental...

2. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ... order, or every combination of materials and treatments is all too often glossed over. This is why it...

3. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ...thrive, in the face of some of Earth’s harshest environments: corrosive pH ranges, crushing pressures, near-melting...

4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ...proteolytically active light chain of BoNT/B into two fragments (BotNT-N and BotNT-C) and joined each fragment...

5. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

   Type

   Blog Post

   Published

   Feb. 19, 2019, 1:36 p.m.

   ...create new programmatic content as well as help our students succeed whether that be academically or on the...

6. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ...to use figures and images to help illustrate our points and break up walls of text. Since our guides are...

7. Transferable Skills Guide: Public Speaking

   Type

   Blog Post

   Published

   April 20, 2023, 4 p.m.

   ...charades, I doubt anyone but me remembers any of the incidents above–and the dumpster noises were just last week...

8. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

   Type

   Blog Post

   Published

   Jan. 11, 2017, 3:30 p.m.

   ...their position and decided to allow individual departments to go ahead with the raises as they see fit, ...

9. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

   Type

   Blog Post

   Published

   Feb. 2, 2018, 3:20 p.m.

   ...the best.” Besides working through the tough assignments, the skills that Susan  finds important for her...

10. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ...can eliminate variability between the different components. When your pathway needs precise fine-tuning ...

11. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...your results are viable for translation to human patients. Picking receptors The receptor you choose is ...

12. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    Published

    June 25, 2020, 1:15 p.m.

    ...Shannon Rinaldi values loving what she does. Her parents told her that if she found something she loves ...

13. Pooled Library Amplifications

    Type

    Blog Post

    Published

    May 30, 2024, 1:30 p.m.

    ... Genome-wide Inhibition Library. DNA and cell amounts After deciding on a cell type, you will need to...

14. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...cytometry has the ability to generate incredible amounts of data from a single experiment. Although there...

15. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ...hoping that your lab mate would have time to make comments on your manuscript. If you give her this slot,...

16. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ...found this to be particularly challenging. As PhD students and postdocs we are used to doing (almost) everything...

17. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ... the challenges you face in your career.  Acknowledgements: I would like to thank fellow Addgenies Joanne...

18. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...Nanobodies also simplify multicolor staining experiments because they don’t require using primary antibodies...

19. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...

20. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...