We narrowed to 837 results for: RING;

1. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   Published

   July 2, 2019, 12:51 p.m.

   ...Fellow at University of Rochester Medical Center. During development, complex genetic programs specify and...

2. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   Published

   April 2, 2019, 1:44 p.m.

   ...non-functional hedgehog exhibit abnormal segmentation during embryogenesis. Typically, regularly spaced denticles...

3. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...activation, only in this case ligand binding would bring the relevant ends together rather than separate ...

4. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ..., and it is exchanged with the gene of interest during the BP or LR reactions. Since the CcdB protein ...

5. Bioinformatics at Addgene

   Type

   Blog Post

   Published

   July 14, 2022, 1:15 p.m.

   ... reviewing and testing several platforms for engineering pipelines, we selected Nextflow, a workflow management...

6. Antibodies 101: The Four ELISAs and When to Use Them

   Type

   Blog Post

   Published

   July 25, 2024, 1:15 p.m.

   ...typically use a competitive or inhibition ELISA when measuring small molecules that cannot bind efficiently to...

7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ... Krüppel associated box (KRAB) is a naturally occurring transcriptional repressor domain which is frequently...

8. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ...of mok results in translation of Mok and Hok, triggering cell death. However, the sok antisense-RNA binds...

9. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...Furthermore, working with Sendai virus requires stringent biosafety measures, thereby increasing the costs...

10. Five Popular Model Organisms

    Type

    Blog Post

    Published

    April 11, 2019, 12:39 p.m.

    ...of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit flies are also...

11. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ... Mary Gearing...

12. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ... Mary Gearing...

13. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...and plasmid backbone. Because you lose some DNA during the gel purification step, it is important to digest...

14. Live and Let Dye: Self-Labeling Protein Tags

    Type

    Blog Post

    Published

    April 16, 2024, 1:15 p.m.

    ...function. If the idea of synthesizing your own ligands brings up bad memories of undergraduate organic chemistry...

15. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    Published

    Oct. 4, 2016, 2:30 p.m.

    ...validating edits.  Mismatch cleavage assays rely on pairing between the edited strand and wild-type strand ...

16. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...in our hands). Take-home message In our hands, delivering Cas9 protein in complex with custom synthetic...

17. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ..., it is much easier to genetically modify cells during the reprogramming process as they are propagated...