We narrowed to 848 results for: STI;
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TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....picking approach to select cells with variant characteristics they wanted, the team identified Archon1, a...
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Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....postdoctoral researcher at the Noble Research Institute, co-instructed the ASPB workshop and authored ... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....themselves and others that most perplexing of questions: “who moved my goat anti-rabbit?!”* *a secondary... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....short-and long-lived proteins, and it can even distinguish between protein variants when used with a variant-specific... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....experiments that requires modest gene activation. Synergistic Activation Mediator (SAM) Description SAM uses... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m.... RNPs, AcrIIA4 reduced off-target editing while still allowing on-target editing (Shin et al., 2017). ... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....and its role in gene expression, though this may still unexpectedly affect nearby genes (Figure 1A-C). ... -
Overcoming the Challenges of Lentiviral Production
TypeBlog PostPublishedAug. 7, 2018, 12:31 p.m....area of the larger vessel to the 10 cm2 dish and adjusting the cell number, plasmid concentration, and experimental... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m.... of pushing them in one by one. It seems very simplistic and it isn’t electronic but it is one of the ... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....Multiple copies of the Mason–Pfizer monkey virus constitutive RNA transport element lead to enhanced HIV-1... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....differentiation) in mouse embryos (Miyatsuka et al.) Distinguish between models for trafficking to the lysosome... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....these systems, though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m.... start producing virus within 24 to 48 hours. Harvesting timepoints will depend on your specific virus... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....your experiment. So, as long as you are able to distinguish your populations from each other on your flow... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....facilitates ubiquitination of the Halo-tagged protein in question, it is targeted to the proteasome for degradation... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m....when a protein is expressed through the use of constitutive and inducible promoters. Inducible promoters... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....such as in the Ultramicroscope. In addition, adjusting the thickness of the laser sheet and using multi-view... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....plasmid-containing bacteria. So… why do some people still use amp? Carb is expensive (relative to amp), and... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m.... and plan for the future of the team. All while still paying bills and having a life outside my job. The... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....labs. Careful tracking prevents us Addgenies from wasting hours of valuable time searching for samples and...