We narrowed to 856 results for: TOR

1. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   Published

   July 21, 2017, 12:48 p.m.

   ...turn genes on or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in ...

2. How to Lead a Great Meeting

   Type

   Blog Post

   Published

   Feb. 9, 2016, 3:30 p.m.

   ... Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research ...

3. Components of CRISPR/Cas9

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   Blog Post

   Published

   Feb. 2, 2016, 5 p.m.

   ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...

4. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   Published

   Aug. 20, 2024, 1:15 p.m.

   ...Purification Protocol Video rAbs in the Addgene repository...

5. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   Published

   Feb. 28, 2023, 2:15 p.m.

   ... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for...

6. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ...excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature...

7. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ... any other required non-CRISPR-associated host factors required for CRISPR processing. For each enzyme...

8. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

9. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...these strains should not be used for long term storage. Additional Resources Review of restriction modification...

10. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ...You never know when you will meet a potential collaborator Some of these people you meet will review your...

11. Antibodies 101: Fab Fragments

    Type

    Blog Post

    Published

    Sept. 5, 2024, 1:15 p.m.

    ...secondary antibodies you already have in your refrigerator will not recognize a Fab fragment. In order ...

12. To Each HIS Own

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    Blog Post

    Published

    March 2, 2023, 2:15 p.m.

    ...affinity purification of human insulin-like growth factor I from culture medium of E.coli. Bio/Technology...

13. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    Published

    Nov. 30, 2021, 2:15 p.m.

    ... you can start by paying attention to how your mentors reach solutions and ask them to explain their logic...

14. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https...

15. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...independently of any other accessory proteins or co-factors, thus allowing for broad applications in a variety...

16. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...applied antibodies and need to dry the membrane for storage, but plan to reprobe it later, strip it thoroughly...

17. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...specific, individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9...

18. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...quickly. Just make sure to incorporate the dilution factor in your calculation from OD to protein concentration...

19. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type

    Blog Post

    Published

    Aug. 6, 2019, 12:55 p.m.

    ...the appropriate primers to detect your targeting vector or gene of interest. 2. Breed your parental mice...

20. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    Published

    Aug. 9, 2022, 1:15 p.m.

    ...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...