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...precise and accessible genome engineering. More elaborate protocols are available in our earlier publications...

...sequencing was considered the gold standard until about 2008 when a new, higher-throughput sequencing-by-synthesis...

...section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts, including...

...conjugate antibodies themselves, the process can be laborious and expensive. In some cases, conjugation methods...

...plasmids may have high copy numbers and reduced metabolic load (Meyer 2009). Figure 1: Idealized ...

... & Corey, D. R. (2021). Reexamining assumptions about miRNA-guided gene silencing. Nucleic Acids Research...

...limitations is the size of GFP: ~240 amino acids or about 28 kDa. GFP and its homologues have a beta-barrel...

...transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration with the Vallabh/Minikel Lab, the Weissman Lab...

... Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits ...

... Brush up on Your Cloning Techniques Learn More about Plasmids in Our Plasmids 101 Series Use CRISPR to...

...reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune...

...which tools to use, and what do you need to know about them to be successful in your experiments? In this...

...starting the selection process. Factors to think about are (1) your experimental conditions and measurements...

...to label the rat brain sample shown in Figure 2A above, Andrews et al. used the following combination of...

...our planet, but we still know collectively little about how they infect and influence the evolution of their...

...target should be near the TSS but you can worry less about sequence optimality because you generally have fewer...

... A., Purkayastha, N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert, D. (2020...

...annotate on this page. Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information ...

... should have a melting temperature 5-10 degrees above the primer and be at least 25 bp away from each ...

...usually severely impaired, and only few reports exist about dye molecules that are transported from one neuron...