We narrowed to 838 results for: cat

1. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

   Type

   Blog Post

   Published

   Oct. 6, 2020, 1:15 p.m.

   ... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

2. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...

3. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4)...

4. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   Published

   July 13, 2017, 2:30 p.m.

   ... from mutations in the gene, and whether any publications have mentioned the gene. The Bloomington Drosophila...

5. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   Published

   Feb. 7, 2019, 2:16 p.m.

   ...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide...

6. Antibodies 101: Flow Compensation

   Type

   Blog Post

   Published

   May 16, 2024, 1:15 p.m.

   ...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...

7. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ...in line 4 of Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed...

8. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ...desktop. The Plasmids 101 eBook is designed to educate all levels of scientists and plasmid lovers and...

9. Tips for arabidopsis transformation

   Type

   Blog Post

   Published

   Oct. 25, 2018, 1:23 p.m.

   ...Agrobacterium tDNA insertion likely precipitated domestication of sweet potatoes (Kyndt et al., 2015)! It sounds...

10. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...other activities. Take care of yourself. Take a vacation alone with your partner – no kids. Get a massage...

11. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...Mbp region of the mouse chromosome. Of the genes located in this stretch of DNA, Ly6a seemed the most likely...

12. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...anticipated outcome, and payoff for each aim. Learn to communicate your story with only the most salient details...

13. Supporting Reproducibility with a Connected ELN

    Type

    Blog Post

    Published

    Jan. 17, 2019, 1:38 p.m.

    ... both accept deposit of data associated with publications into one of five general purpose open source...

14. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ...staining. Direct ELISAs also lack the signal amplification afforded by secondary antibodies and thus are...

15. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ... written up in mSphere. A post-meeting survey indicated that the program was largely a success, but also...

16. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...many cell types and often results in biallelic modifications. Most commonly, DSBs are repaired by the nonhomologous...

17. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ...created by making some or all of the following modifications: 1) increased concentration of Taq polymerase...

18. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...interested in using CRISPR to develop therapeutic applications that better human health.     References Cong...