We narrowed to 845 results for: ins;
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TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....regulate transcription. Red flame plasmid pDawn contains the blue-light sensing protein YFI. When light...
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How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....careers Plasmids CRISPR Viral vectors Fluorescent proteins ... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....AAVrg while other pathways such as those in the brainstem were not. Others have noted a lack of transduction... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m.... using your single color controls (Figure 1A), instead of using your actual samples. They may also be ... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog PostPublishedJan. 30, 2020, 2:15 p.m....which often focuses more attention on the results instead of the methods. Unreported flexibility and questionable... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....They all found the support of the group to be intrinsic to their success. Their stories made it clear ... -
Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m.... Eric J. Perkins... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....up in both science journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....oligonucleotide (ssODN) template supplied by Ma et al., but instead from the mother’s wild type allele (Figure 1). ... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m....addition to the 20 bp guide sequence, the crRNA also contains a ~20 bp repeat region on its 3’ end. This repeat... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....scientists can receive recognition for their efforts, inspire future research, and increase their impact. Open... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m.... with very little text on the slides and notes instead of written speeches. When you’re doing all that... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....Novartis Oncology. Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc in January... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....seriously. As I make my rounds speaking at research institutions and at scientific conferences, I see that few... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....organizations, libraries, youth groups, and faith institutions. The conversations often move quickly into sensitive... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....since these processes are mediated by other viral proteins), but the newly released RABV cannot infect any... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m....certain cell types. Botond Roska’s lab at the Institute of Molecular and Clinical Ophthalmology Basel ... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....The day has arrived; you’ve painstakingly cared for your packaging cell line, prepped your DNA, transfected... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m.... decide what they’d like to work on later. For instance, AstraZeneca posts most of its positions all at... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog PostPublishedSept. 13, 2018, 1:55 p.m....genome-wide CRISPR screen presented in Morgens et al. contains three types of guides: 1) targeting guides (blue...