We narrowed to 904 results for: CAN

... been motivated to do anything and everything we can to help find a cure -- and quickly. Normally drug... approach is to discover key human proteins that can be targeted (hopefully by existing therapeutics that...these experiments, there's so much to do that we can use the whole science community's help. You could... but one are in a pLVX vector background, so you can package them into lentiviral vectors if you need ...FedEx guy has been the best: Thanks, Todd! As you can imagine, it’s also been quite the administrative [email protected] with your order number and so it can be prioritized. ...Arabia -- as well as closer to home in the US and Canada. In fact, we received so many requests (175 and...

... light exposure can also lead to cellular phototoxicity, and experimental success can be affected by both...biologists over the last two decades, but their use can be limited by a few caveats. Since fluorescent proteins...and showed that these three luminescent proteins can be used together in cells. As Nano-lanterns are not...fluorescence, so higher concentrations of the compound can be added to the media. Cellular esterase then converts...optogenetic tools, where light used for excitation can cause unintended activation of the optogenetic system...proteins (FP) require external light activation, you can’t use fluorescence to monitor processes directly affected... physiology in your system. Optical sectioning cannot be conducted with luminescence imaging since there...

...Addgenie expertise we can. We even have a Slack channel just for asking people if they can write or review ... is our Help Center, help.addgene.org, where you can easily search articles answering all of our frequently... our depositors and customers are asking. So how can you get involved? Keep asking your burning questions...and tell us what you want to know more about! You can also suggest blog topics or even reach out about ...support and advance the scientific community, and we can only do that with your help.     Additional Resources...

...protocol can save weeks of trial-and-error. A recommendation for the correct statistical test can save a...Spain and works in the field of genetics and cancer. You can follow her on Twitter at @mahesa2504       ...community where both brief and long-term collaborations can be easily started and easily credited. And nothing...scientific research. It’s completely free, of course. You can find more information about us and what we do in ... based in Spain and works on climate change. You can follow him on Twitter at @IgnacioAmigoH     María...while most of the world was locked down, a South African company wanted to test if a type of antibodies ...

...differentiation To understand how each human TF can contribute to differentiation, the Zhang lab transduced...Similar to the Human TFome Library, the MORF library can be used with other selection and screening mechanisms...library webpage. Disease models Good disease models can often be difficult to generate, in part because of... dysregulation. A targeted TF screening approach can not only lead to a better understanding of disease...simulate a disease in a model system. TF screens can be designed to identify custom TF programs to acutely...and GFP fluorescent controls. Alternatively, you can order individual plasmids from the library to further...Individual cellular fates were then matched with candidate TFs responsible for that differentiation.   ...

...protein interacts with that one promoter can tell you, it can be easy to get lost in the details. The ...like stem cell biology where questions of ethics can have profound impacts on the development of applications...and appreciate the importance of ethics, the topic can often take the back burner at scientific conferences...international borders. While questions of ethics can be daunting because they have no clear cut answers...stem cell community with their research, I think I can speak for Addgene when I say that I'm glad we're ... important questions that the scientific method cannot address. Listen to Our Podcast Interview with Stem...

...publishing research papers can be long and unpredictable. Fortunately, you can submit plasmids at any point... your plasmids! You can make your deposit available before publication and we can update the plasmids’...is posted in order to support these goals. And we can update your plasmids’ citation to the final journal...unresolved sequencing issues or if you’ve asked to cancel the deposit due to experimental problems, legal...article is published. As much as we’d like to, we can’t keep up with every paper that comes out! So, before...

...attorneys with a biology background were in demand. Q: Can you tell me a bit about your job currently? What ... the business world from the mindset of a lawyer can bring a new perspective to an organization. I'm used...an organization. I think these are things that I can bring to Addgene -- that comfort with saying, “if... and judgement to the organization and see how I can help. Q: What do you enjoy doing outside of work?...

...the CTD can get cozy with DNA. S. pyogenes Cas9 recognizes the PAM sequence 5’-NGG-3’, where N can be any...for genome engineering, and studying its structure can help us understand the parameters of CRISPR experimental...Other techniques, like electron microscopy and FRET, can offer more information on these regions.   Cas9’s...reflecting the less stringent recognition mechanism that can tolerate mismatches in this region. The crystal structure...any base. In Figure 5, we can see why: two arginines make base-specific hydrogen bonds to the guanines...paired to each other? At this point, the protein can switch between the closed and open conformations ...with the rest of Cas9 and is highly flexible; it can rotate about 140 degrees between inactive and active...

...expertise and skills of many of my colleagues, I can also orchestrate the formation of high-functioning-but-temporary...to have their own specialties. This type of team can exist indefinitely. If you’re looking for this type...biostatistician, and that one golden-handed technician who can do a certain difficult assay with her eyes closed...the skills are not cherry-picked. We take what we can get since participation on such a team is strictly...without such an association, start one! These teams can offer a sense of solidarity you’re going to crave...about this.” Sometimes the common roles of a leader can be successfully doled out to multiple people: someone...people actually like meetings, but run well, meetings can be a critical part of any successful team. For reasons...