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We narrowed to 892 results for: CAN

Showing: 421 - 430 of 892 results

  1. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...publishing research papers can be long and unpredictable. Fortunately, you can submit plasmids at any point... your plasmids! You can make your deposit available before publication and we can update the plasmids’...is posted in order to support these goals. And we can update your plasmids’ citation to the final journal...unresolved sequencing issues or if you’ve asked to cancel the deposit due to experimental problems, legal...article is published. As much as we’d like to, we can’t keep up with every paper that comes out! So, before...

  2. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    Published
    Aug. 3, 2021, 1:15 p.m.
    ...attorneys with a biology background were in demand. Q: Can you tell me a bit about your job currently? What ... the business world from the mindset of a lawyer can bring a new perspective to an organization. I'm used...an organization. I think these are things that I can bring to Addgene -- that comfort with saying, “if... and judgement to the organization and see how I can help. Q: What do you enjoy doing outside of work?...

  3. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...the CTD can get cozy with DNA. S. pyogenes Cas9 recognizes the PAM sequence 5’-NGG-3’, where N can be any...for genome engineering, and studying its structure can help us understand the parameters of CRISPR experimental...Other techniques, like electron microscopy and FRET, can offer more information on these regions.   Cas9’s...reflecting the less stringent recognition mechanism that can tolerate mismatches in this region. The crystal structure...any base. In Figure 5, we can see why: two arginines make base-specific hydrogen bonds to the guanines...paired to each other? At this point, the protein can switch between the closed and open conformations ...with the rest of Cas9 and is highly flexible; it can rotate about 140 degrees between inactive and active...

  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...depicted on the left) is ~5.8 kb in length. The plasmid can exist on as a dimer, depicted on the right, with ...bacterial cells compared to monomers, although this can be prevented via antibiotic selection (Crozat, 2014...instances, please note that the supercoiled dimer band can sometimes overlap with the band corresponding to ...Electrophoresis (CGE) analysis Another technique that can detect multimers is capillary gel electrophoresis...laser-induced fluorescence (CGE-LIF) for detection and can provide data on the quantity of various topologies...strain that is deficient in recombinase protein RecA can reduce the frequency of multimerization, and in some...temperature such as 30°C or optimizing the incubation time can help maintain cultures in log phase growth. In a ...

  5. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...expertise and skills of many of my colleagues, I can also orchestrate the formation of high-functioning-but-temporary...to have their own specialties. This type of team can exist indefinitely. If you’re looking for this type...biostatistician, and that one golden-handed technician who can do a certain difficult assay with her eyes closed...the skills are not cherry-picked. We take what we can get since participation on such a team is strictly...without such an association, start one! These teams can offer a sense of solidarity you’re going to crave...about this.” Sometimes the common roles of a leader can be successfully doled out to multiple people: someone...people actually like meetings, but run well, meetings can be a critical part of any successful team. For reasons...

  6. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...to be low copy number. Conversely, plasmids that can initiate DNA replication independently are said to...AT-rich regions of DNA. In a test tube, the DNA helix can be separated by DnaA alone, as long as there are ...occupied by ATP-bound DnaA, the proteins cooperatively can separate the DNA strands. Created with BioRender.... available to bind DnaA. Fun fact! As far as we can tell, DnaA doesn’t need to hydrolyze ATP to do its...from the population, while replicating too quickly can slow cell growth or even kill the host cell. For ...replication of pSC101 depends on host-encoded proteins, it can be regulated indirectly via the same mechanisms that... to keep replication in check, high copy numbers can allow RepA to link iterons from two different plasmids...

  7. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...results in two DSBs that can be repaired by NHEJ restoring mCherry expression. Cells can then be sorted using...editing event. GFP positive cells can be sorted and have a significantly higher frequency of base edits ... created an array of base editing reporters that can help you do just that. Monitoring APOBEC and Cas9...due to a frameshift. Restoration of fluorescence can only occur through editing by a APOBEC-Cas9n-UGI ...Fluorescence-activated cell sorting (FACS) and the editing efficiency can be easily determined by comparing cells expressing...editing event would result in GFP fluorescence that can be visualized and sorted via flow cytometry. ...mutated start codon to initiate protein expression, it can be used for an array of detectable proteins including...

  8. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ... functionalities, it can be difficult to figure out all the ways a given ELN can make your lab life easier... full activity on a project. Can you meet the ELN support team? We cannot stress enough how important ... It is important to know in which ways your data can be exported or printed from the ELN when needed. ... reporting on a project – printing your data out can be required every once in a while, so it is good ...your team. Do you need to make sure your research can be patented? I.e. is automated time stamping (detailed...the times and dates of your actions. These records can take the form of written comments, added files, notes...easier. If you can talk to an ELN support team directly, they should be able to walk you through your ...

  9. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ... recombinant protein expression. Many challenges can arise when over-expressing a foreign protein in E...restriction/methylation system. This means the strain can neither restrict nor methylate DNA. dcm: Similarly...works well for most experiments, the lac promoter can “leak,” meaning that a low level of expression exists...mostly a problem for toxic protein products, which can prevent the culture from reaching the desired density...any) protein expression? Low expression outcomes can result from variety of sources, so fear not—there...not—there are a few simple troubleshooting measures that can help get you back on track: Compatibility: Double-check...Growth Media: Changing media is tricky, because there can be a trade-off between growth rate and protein quality...

  10. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...COVID-19 and coronavirus collection page, where you can find plasmid tools to accelerate your COVID-19 research.... Download the blue plasmid background.     You can find all of the plasmids that Addgene offers by visiting... visiting our collections page. Here, you can browse plasmids by field, organism, research technique, ...from Addgene are shipped as bacterial stabs? You can learn about what format your material will come as...
Showing: 421 - 430 of 892 results