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We narrowed to 928 results for: Gene

Showing: 421 - 430 of 928 results

  1. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...address this issue by developing generic strategies for detecting cloned genes (Munro and Pelham, 1984). The...the human c-Myc gene (Evan, et al. 1985). Myc was one of the earliest identified oncogenes and was a target...tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the.... However, it was (and still is) infeasible to generate highly specific antibodies for every potential...explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic peptides corresponding...epitope within that region. You’ll notice that “generate a reliable antibody for protein tagging approaches...

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression is induced by...a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact Cas9 ortholog from Staphylococcus... Figure 1. (A) SARS-CoV-2 RBD DMS Library generation and sequencing. From Starr et al Cell., 2020....scFV library and characterized them by ELISA, homogeneous time resolved fluorescence, and suspension bead...synthetase complex, the team investigated whether the generated antibodies could bind these aaRSs. They found ... Gräslund lab plans to continue their work by generating antibodies for additional aminoacyl tRNA synthetases...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...

  3. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...22,000 protein coding genes in the human genome with fluorescent proteins. For each gene, we needed to design...way that prevents re-cutting but does not disrupt gene expression. (See section below.) For guide design... Zuckerberg Initiative. We hereby announce the general availability of new a tool for CRISPR scientists...analyses, but it also allows you to download any generated info for your own purposes, or delete the originals...at existing tools. As has been written here on Addgene, there has been a Cambrian-like explosion of software...that it can be used in the rank equation above. Generally, the greater this distance between the cut and...sequence inserted by HDR. Although this practice is generally advised, there is little detail published on how...

  4. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...guides and an NgAgo expression plasmid to edit a gene in vivo. Try NgAgo for Yourself On the other hand...were having difficulty achieving indel formation (gene disruption) with this new technology. To dig more...asked if they could detect indels (an indication of gene disruption ability) with NgAgo, one respondent out... do not necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions...the water. On the bright side, successful indel generation and epitope knockin was achieved by a few respondents...Twitter - Tweet suggestions and tips with #NgAgo The Addgene Blog - Leave suggestions and tips in the comments...comments section below or contact blog@addgene.org if you'd like to write a more detailed post It may take...

  5. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...adeno-associated viral vectors) are commonly used for in vivo gene delivery due to their low immunogenicity and range... the potential “sticky end” transgene insertion mediated by Cpf1-generated overhangs. Exploring the efficiency...suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et ...in AT-rich genomes. Additionally, whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1...certain tissues. However, these vectors have a low genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits...27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver, Benjamin P., et al. "Genome-wide...

  6. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors To focus on a particular gene or protein, check out our gene-specific biosensors. These constructs...Visualization Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent... to the molecule it detects. This change then generates a reporter signal. Reporter signals may be electrochemical... OR enzyme activity. Small molecule biosensors Addgene has many sensors that measure the concentration..., and voltage across membranes. These sensors generally follow the same pattern described above - a sensing...fluorescent molecule   Additional Resources on the Addgene Blog Check out the Rosella Autophagy Sensor Read...

  7. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...used to improve CRISPR-based activation of target genes, but we’ll save that application for another day...protein-tagging system for signal amplification in gene expression and fluorescence imaging.” Cell 159(3...mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at very low levels due...protein-by-protein basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames,” it...PMCID: PMC4252608. Additional Resources on the Addgene Blog Sign Up to Receive the Fluorescent Protein...about Small Molecule Fluorphores Resources on Addgene.org Find Fluorescent Protein Empy Backbones Visit...

  8. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...Truncated gRNAs for Regulating Gene Expression and CRISPR 101: Epigenetics and Editing the Epigenome (plus...Joining and PITCHing MMEJ as an Alternative Route for Gene Editing Day 9: Precision editing: Single Base Editing...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...flame for its adenine base editing capabilities. Epigenetics: CRISPR has made it possible to design programmable... blue flame plasmids covering three different epigenetic effectors. The diversity of tools is especially...plus bonus podcast with Addgene Advisory Board member Alex Chavez) Day 11: Pooled libraries: Genome-Wide...

  9. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...and caveats of Cre usage: Vagaries of conditional gene targeting. Schmidt-Supprian M, Rajewsky K. Nat Immunol...activity regulation. Heidmann D, Lehner CF. Dev Genes Evol. 2001 Sep;211(8-9):458-65. doi:10.1007/s004270100167...specific recombination site for each enzyme, were generated to test recombination efficiency. When expressed....1111704108. Epub 2011 Aug 9. PubMed. Find the Plasmids @Addgene: Plasmids for KD, R, B2, and B3 recombinases from...

  10. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...attach the gene of interest to either the N-or the C-terminus. Even if a researcher’s gene of interest...researchers as we could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited...is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection...collection with over 300 backbones alone,” said Addgene’s Lianna Swanson, who has been working with members...the scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great ...
Showing: 421 - 430 of 928 results