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...considering using this PEI Calculator. AAV packaging typically requires transfecting three plasmids at specific...

... plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to create...

...February 13, 2021 Westenberg, a Professor of Biological Sciences at Missouri University of Science and...

...streaking to a single colony to DNA extraction and quantification so that you can focus on what you want to do...

...Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes. You can follow him...

...Once your request is received, we’ll review for technical and legal compliance, and then send you an email...

...wearestemsquad #festivefun #phdlife #phdtips #sciencecommunication #sciencedaily #instascience #ladies #research...

...Doench (Guest Blogger) Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making...

...Hiroshima University. “However, there had been no systematically established way of making an all-in-one vector...

...plasmids, store your plasmids or viral items, technical support, and more. ...

...curated information about AAV usage in research applications. Scientists around the world can upload AAV ...

...KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is...

...as links to the Zhang Lab's Nature Protocols Publication describing them here. References 1.Ran, F. Ann...

...of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this viral sequence in the assay...

...right path. The frequent Zoom meetings helped me communicate my ideas. I was able to express my visions for...

...Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley,...

...approach." Proceedings of the Royal Society B: Biological Sciences 272.1577 (2005): 2117-2126. PubMed PMID...

...course of scientific research. What was once a complicated process of requesting and sharing plasmids has...

... a Cas9 nickase and two gRNAs.  For nickase applications, a common question is: how should the gRNAs ...

...providing an avenue for temporary/reversible CRISPR modification. In addition to these subpages, our updated ...