We narrowed to 904 results for: CAN

...expertise and skills of many of my colleagues, I can also orchestrate the formation of high-functioning-but-temporary...to have their own specialties. This type of team can exist indefinitely. If you’re looking for this type...biostatistician, and that one golden-handed technician who can do a certain difficult assay with her eyes closed...the skills are not cherry-picked. We take what we can get since participation on such a team is strictly...without such an association, start one! These teams can offer a sense of solidarity you’re going to crave...about this.” Sometimes the common roles of a leader can be successfully doled out to multiple people: someone...people actually like meetings, but run well, meetings can be a critical part of any successful team. For reasons...

... functionalities, it can be difficult to figure out all the ways a given ELN can make your lab life easier... full activity on a project. Can you meet the ELN support team? We cannot stress enough how important ... It is important to know in which ways your data can be exported or printed from the ELN when needed. ... reporting on a project – printing your data out can be required every once in a while, so it is good ...your team. Do you need to make sure your research can be patented? I.e. is automated time stamping (detailed...the times and dates of your actions. These records can take the form of written comments, added files, notes...easier. If you can talk to an ELN support team directly, they should be able to walk you through your ...

...to be low copy number. Conversely, plasmids that can initiate DNA replication independently are said to...AT-rich regions of DNA. In a test tube, the DNA helix can be separated by DnaA alone, as long as there are ...occupied by ATP-bound DnaA, the proteins cooperatively can separate the DNA strands. Created with BioRender.... available to bind DnaA. Fun fact! As far as we can tell, DnaA doesn’t need to hydrolyze ATP to do its...from the population, while replicating too quickly can slow cell growth or even kill the host cell. For ...replication of pSC101 depends on host-encoded proteins, it can be regulated indirectly via the same mechanisms that... to keep replication in check, high copy numbers can allow RepA to link iterons from two different plasmids...

...results in two DSBs that can be repaired by NHEJ restoring mCherry expression. Cells can then be sorted using...editing event. GFP positive cells can be sorted and have a significantly higher frequency of base edits ... created an array of base editing reporters that can help you do just that. Monitoring APOBEC and Cas9...due to a frameshift. Restoration of fluorescence can only occur through editing by a APOBEC-Cas9n-UGI ...Fluorescence-activated cell sorting (FACS) and the editing efficiency can be easily determined by comparing cells expressing...editing event would result in GFP fluorescence that can be visualized and sorted via flow cytometry. ...mutated start codon to initiate protein expression, it can be used for an array of detectable proteins including...

... recombinant protein expression. Many challenges can arise when over-expressing a foreign protein in E...restriction/methylation system. This means the strain can neither restrict nor methylate DNA. dcm: Similarly...works well for most experiments, the lac promoter can “leak,” meaning that a low level of expression exists...mostly a problem for toxic protein products, which can prevent the culture from reaching the desired density...any) protein expression? Low expression outcomes can result from variety of sources, so fear not—there...not—there are a few simple troubleshooting measures that can help get you back on track: Compatibility: Double-check...Growth Media: Changing media is tricky, because there can be a trade-off between growth rate and protein quality...

...information. Our agility as a nonprofit resource can prove to be vital during this period of constrained...isn't lost. Universities and research institutions can turn to Addgene as a trusted partner for archiving... difficult to maintain or access. These services can be listed as direct costs from a CRO in grant applications...commitment to collaboration and resource sharing, we can navigate this difficult period together. The future...

...COVID-19 and coronavirus collection page, where you can find plasmid tools to accelerate your COVID-19 research.... Download the blue plasmid background.     You can find all of the plasmids that Addgene offers by visiting... visiting our collections page. Here, you can browse plasmids by field, organism, research technique, ...from Addgene are shipped as bacterial stabs? You can learn about what format your material will come as...

...this post, I’ll describe how rabies virus (RABV) can be used in the brain to visualize how neurons are... this, cell type specificity of the starter cell can be achieved by using EnvA-pseudotyped RABV, and only...in user-defined cells. For example, a researcher can deliver Cre-dependent TVA to Cre-expressing neurons...it is supplied in trans. Monosynaptic RABV spread can be achieved by modifying the wild-type RABV genome...Wickersham et al., 2007). Thus, RABV transsynaptic spread can be controlled by selectively supplying the G protein... Once in first order neurons, the G-deleted RABV can divide and replicate (since these processes are mediated...RABV monosynaptic tracing is a powerful tool that can be used to reveal brain circuitry, and continue to...

...Bimolecular Fluorescence Complementation (BiFC). It can provide a qualitative or quantitative readout of ...of the interaction in vivo or in vitro, and can be used for measuring protein expression or localization... When Protein A and B interact, the FP fragments can assemble the full structure of the fluorescent protein...from a different base FP (for example, EBFP2(1–10) can be used with GFP(11)). Some of the articles linked...color labeling with exogenous fluorophores, which can even be bright enough for single-molecule tracking...interest. Remember that the FP fragments themselves can sometimes interact in cells and produce background...termini are important for their interaction. You can read our previous blog post for more on BiFC principles...

...mediate A•T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially... in action they bind DNA so that the TadA domain can edit the short single-stranded DNA stretch that is...catalytically impaired D10A nickase from ABE7.10 can be a problem. Therefore, the authors created ABE8...protospacer adjacent motif (PAM) made up of 5′-NGG-3′ (“N” can be any nucleotide base, followed by 2 guanines). ... in primary human cells  Cell therapy approaches can use base editors to correct mutations in stem cells...which cause sickle cell disease and 𝛽-thalassemia, can be mitigated with the expression of fetal hemoglobin...plays a role for off-target editing effects. ABEs can be introduced into cells as a plasmid or as mRNA ...