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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 898 results for: CAN

Showing: 861 - 880 of 898 results

  2. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...follows a similar path to base transition editing, we can expect several generations of optimization before...standard tools. Additionally, aspiring genetic editors can now compare the advantages of base editing and prime...processes that would cause DSBs, and its inclusion significantly reduced indels generated by CGBEs. What's next...

  4. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...reagents are all ways in which the scientific community can support repositories. Repositories are essential ...this virus. One such example is cell lines. The American Type Culture Collection (ATCC) is a private, nonprofit...

  5. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ... that many more experiments are needed before we can consider clinical applications for germline editing...biologists and non-biologists alike. Given the public significance of this research, we have the obligation to ...

  6. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...with postdocs at other institutions to see if we can similarly convince them to go ahead with raises. ...level, in the case of some public institutions) to cancel plans to raise salaries. What are institutions ...expecting a salary increase have had that increase cancelled. Rounding out the remainder, 2% of postdocs who... are continuing with their plans, and 17 have cancelled their plans. 2 of those raising - the University...Illinois and the University of Michigan - initially cancelled their plans, but have since reversed their position...announced that they were reversing their plans to cancel raises). A further 9 institutions, whose plans ... In addition, PIs at some of the institutions cancelling plans are going ahead with raises anyway, including...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...been a privilege to find and share what stories we can, and to hear the stories others have to tell.  "Also...

  9. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ... focused group of participants. More information can be found at http://futureofresearch.org/advocating-for-science-boston...and keynote by former congressman and CEO of the American Association for the Advancement of Science, Rush...solutions. The event also culminated in a joint American Society for Biochemistry and Molecular Biology...keynote address by former congressman and CEO of the American Association for the Advancement of Science, Rush...

  12. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...real value here is seeing them all in one place. Can we address problems in scientific publishing without...posits the way science is being done has changed significantly over the last decade. He describes the detection...

  13. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ... from reaching their targets. The AAV9 serotype can breach the BBB following injection into the bloodstream...PHP.B to reach the CNS; yet LY6A is an unlikely candidate. It was discovered as an antigen upregulated on...this stretch of DNA, Ly6a seemed the most likely candidate due to its strong linkage to the PHP.B phenotype...variants could unintentionally limit the utility of candidate capsids.  Are you interested in using AAV-PHP....

  14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ... from just one lab, which limits how broadly one can interpret our analysis of COVID-19’s effect on scientific...months, pausing or terminating employment for a significant fraction of the workforce. Wet lab scientists...exponential decay model, with dips during weekends and significant holidays. Figure 4: Plasmid request trajectories...or labs placing orders for paper x on date t. We cannot use individual plasmid orders, because when a PI...Institute and Harvard University. Max Shen is a Ph.D. candidate at MIT. His research uses applied machine learning...

  15. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... ITAM phosphorylated. To demonstrate that pYtags can be generalized to other RTKs of interest, they create...targets. Figure 6: A) Schematic of BeadScan platform for screening candidate biosensor variants for performance...tdTomato), they produce a temperature sensor that significantly outperforms previous designs. B-gTEMP is excitable...optimal conditions). While B-gTEMP itself is a significant improvement over previous temperature indicators...

  16. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated DNA not degraded, cell cannot methylate DNA... Mutation in galactose metabolism pathway Cells cannot grow on galactose only gyrA, gyrA96 DNA gyrase...   lacY Lactose permease inactivated, lactose cannot be taken up by cell mcrA, mcrBC Inactivation ...

  17. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...were less significant than when non-targeting guides are used as controls. Less significant p-values lead...genes involved in the production of a cell surface glycan that’s required for ricin uptake. The ricin screen...guides and may more accurately determine the significance of hits in non-growth screens. Off-target cutting...of on-target activity. However, there was no significant difference in depletion of ricin sensitizer genes...

  19. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    Published
    March 17, 2021, 2:40 p.m.
    ...an organization that raises money for childhood cancer through the sale of art. About two years ago, I...Northeast Human Resources Association, Association of American Medical Colleges and the Society for Human Resource...501c3 public charity raising funds for childhood cancer through the sale of art. Past volunteer appointments...

  20. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...expedite research. We recognize, however, that we can’t do it all ourselves. Therefore, in this blog post... write for the Addgene blog simply email us. We can’t guarantee that all those who sign up will be able...
Showing: 861 - 880 of 898 results