We narrowed to 886 results for: URE
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TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....number of researchers, I had high hopes of securing tenure in a British or Irish University where I would ...
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Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog PostPublishedMarch 6, 2014, 7:12 p.m....Time-bound. It is a good idea to take larger goals (e.g. Figure out what I want to do after I get my PhD) and break... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....of the reporter mRNA is labeled by PCP-mCherry (Figure 2). The 3’ UTR also contains a CAAX sequence to... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....generating your own fly lines. In the meantime, be sure to browse the publication references and web links... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m.... should improve the specificity of influence measurements by reducing photostimulation of the axons and... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....fluorescence after cleavage by executioner caspases. Failure for cells to die or cells dying at the wrong time... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....and numeracy on perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog PostPublishedMay 3, 2018, 1:48 p.m....produced 92% and 87% mCherry protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m.... time to choose your own protein purification adventure. You want to purify your favorite protein (YFP... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....plasmid sharing has certainly taken us on many adventures...From moving office locations three times, to... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m.... brain, and holds promise in the development of future therapies. Our popular plasmid encoding a Cre dependent... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....connections, in a powerful way. Using brain structure to understand brain function So far scientists... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....cell, this system is clearly also robust enough to ensure high levels of gRNA cleavage. Xie et. al constructed... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....and train the participants in skills useful for future mentoring relationships. If you would like to... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m.... understand what you’re communicating. While I’m sure that there are loads of ways psychologists define... -
The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....much time does it take to make a plasmid? Figure 2: Survey results from a Twitter poll run in 2023... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m.... of protein of interest the membrane. Figure 1: Electron microscope images showing ultrastructural... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....then digested with the Type IIS enzyme, and the mixture is ligated following a heat inactivation step. ... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m.... transcriptional and translational termination features with loxP sites on either side (often called a... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....plasmids as well as transfer plasmids. This concept ensures safe handling and expression of these vectors when...