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We narrowed to 892 results for: CAN

Showing: 441 - 450 of 892 results

  1. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...clone can then be monitored over time by sequencing the barcodes in the population (all barcodes can be ...of cells with specifc, easily read sequences that can be used to measure their clonal abundance before ...tumor xenograft models. She hopes that the library can be used to identify appropriate, non-overlapping ...development - such that initial resistant populations can be eradicated up-front quickly. This approach is ...25849130. 2. Hata, Aaron N., et al. "Tumor cells can follow distinct evolutionary paths to become resistant...resistant cancer cells exist prior to drug treatment. They infected a non-small cell lung cancer cell line...tool to look at a variety of questions surrounding cancer development, metastasis, and drug resistance, Bhang...

  2. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...smegmatis (Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...system for genome engineering is the fact that Cas9 can be easily programmed to make a DNA double strand ...frameshift mutation. Of some importance, the deletion can be less heterogeneous when constrained by sequence...loading of Ku and normal NHEJ activity. Other factors can also influence NHEJ activity. For example, repressing... flank regions of hundreds of base pairs or more can simultaneously introduce a pair of chromosome breaks...containing compatible overhangs is available. Cas9 can also be altered to generate a targeted single strand...and Biophysics and the Lineberger Comprehensive Cancer Center at UNC Chapel Hill.   References Bennardo...

  3. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...Additionally, you can upload your own images to the Biorender workspace. Free Biorender accounts can store 5 workspaces...color and size of icons are adjustable. New icons can be requested, although there’s no guarantee Biorender...informal settings like lab meeting presentations. You can download larger images without the watermark, which...then clicking the “Usage rights” dropdown menu, you can search for images “Labeled for reuse with modification...Google Image Search results includes images that can’t be reused without attribution, despite filtering...

  4. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ... of the AAV we have released so far in 2019. You can also browse our entire AAV inventory! Our new AAVs...fluorescence of the GCaMP7 family of calcium sensors and can be used for imaging of small neuronal processes like...Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction with optogenetic activators...AAV These fluorescent protein expression vectors can be used to visualize cells and to quantify targeting...expression to GABAergic interneurons. These vectors can also be used as the control vectors for experiments...

  5. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...victoria the functionalized nanobodies described here can be used to target any protein fused with GFP, YFP...vicinity with biotin. The biotin-labeled proteins can then be isolated and analysed. In this work, the ...School have developed a group of plasmids (here) that can be used to label all proteins within a radius of ...Tyrosine) with biotin. These biotin-labeled proteins can them be analyzed by mass spectrometry allowing a ...composition of proteins found at the telomere, and hope it can be used to expand our understanding of proteome localization...MarathonRT, isolated from Eubacterium rectale, significantly outperforms other RTs in this regard and is ...

  6. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...sequences of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both ...occurs which cuts the DNA.3.) The released transposon can now bind a strand of DNA with a TA dinucleotide (...transposon containing the gene-expression cassette that can translocate within the genome. The Sleeping Beauty...generation of stable transgenic cell lines which can robustly express your gene-of-interest, either constitutively...Additionally, in 2014 researchers at MD Anderson Cancer Center presented results from a clinical trial ...

  7. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...mismatches that can nevertheless still lead to activity has been established, and can be used to identify...provide updates on how best to design gRNAs. You can also find validated gRNAs for your next experiment...Datatable. A more extended discussion of these subjects can be found in two recent review articles (Doench et...importance (target should be near the TSS but you can worry less about sequence optimality because you ...variants thereof offers additional PAM options that can greatly expand gRNA options. Created with ... in vitro transcription, or lentiviral delivery) can affect the accuracy of a predictive score, as well...computational prediction is ever perfect, but this can decrease the number of guides one needs to test in...

  8. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...editing and twin prime editing can integrate DNA cargoes of 80–100 bp, PASSIGE can integrate cargoes over a ...concentration and purity of the target molecule, which can be problematic for low-abundance targets or natively...plunge freezing By Rachel Leeson Cryo-EM samples can be damaged at the air-water interface (AWI) during...small proteins that naturally adsorb to the AWI, can protect cryo-EM samples from damage (Abe & Lim, 2024...air-water interface (AWI) before vitrification, they can be completely denaturated (left) or complexes may...of dCas9 fused to a DNA methyltransferase — that can drive lasting transcriptional silencing (Nuñez et...methyltransferase for epigenetic silencing, which can be toxic, CHARM uses a cofactor fused to a histone...

  9. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...CRISPR components for plant genome engineering CRISPR can be used to knockout, activate or repress target genes...required. As with other model systems, multiple gRNAs can be expressed to modify several genomic loci at once...dCas9-VP64) or repressors (dCas9-KRAB or dCas9-SRDX) can also be used to activate or repress target genes ...result in a failed experiment. CRISPR components can be expressed stably or transiently depending on the...method and cell type in question. CRISPR components can be delivered and expressed transiently using a standard...information on Agrobacterium-mediated transformation can be found in this blog post. The pDGE Dicot Genome...transformation in plant cells. Agrobacterium tumefaciens can be used as a vehicle to deliver your gene of interest...

  10. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...How can I quickly check if the cloning of my oligo into a backbone like PX330 worked? A12: You can quickly...newest CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR", short-hand...concept of the double nickase system is that you can express two different chimeric gRNAs with the Cas9... cuts 3–4 bp upstream of the PAM sequence. There can be some off-target DSBs using wildtype Cas9. The ...Tips for using homologous recombination (HR) Q7: Can you summarize HR template vector design guidelines...shorter. Q10: What is the maximum amount of DNA that can be inserted into the genome using CRISPR/Cas for ...reference for using ssODN as HR Template? A11: You can take a look at Soldner et al. (2011) which uses ssODN...
Showing: 441 - 450 of 892 results