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We narrowed to 904 results for: CAN

Showing: 441 - 450 of 904 results
  1. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...places they can’t reach on their own. The antibody’s heavy-chain constant domains, or Fc region, can be bound...isotypes. Binding to complement system proteins, which can initiate a cascade that helps attack extracellular...useful to know the isotype of an antibody so you can select an appropriate isotype control. An isotype...There are two IgA subclasses: IgA1 and IgA2.  You can learn more about isotype classes in our Antibodies... constant domain gene cluster. DNA recombination can delete intervening heavy-chain constant domain genes... antibodies Read about other techniques that you can use antibodies for in our Intro to Antibodies post...
  2. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ... indicator. Archon1 can embed itself into the cell membrane where cell voltage can then be measured. Archon1...rapidly screen thousands of proteins for one that can report electrical activity via imaging. They took...nature, but developing them into optogenetic tools can be difficult because this requires detailed structural...interactions in cells because the protein binders can be customized for protein visualization, relocation...these photo-reversible protein-protein interactions can control subcellular localization processes in vivo...
  3. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact both...or chemically reactive side chains, all of which can help determine structure and function relationships...relationship between structure and function…if you can get your protocol to work. Or, to quote Ryan Mehl...robust and universal techniques that researchers can easily implement in their own labs. They provide ...bulletin board on their website specifically so people can talk about GCE. In fact, their first protocol is ...proteins, you’re probably quite interested in the canonical 20 amino acids. But in your quest to learn more...code expansion (GCE): techniques in which non-canonical amino acids (ncAAs, also sometimes called unnatural...
  4. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...provides the widest tropism or range of cells a virus can infect. Note: While both lentiviruses and γ-retroviruses...modify tropism. Briefly, different envelope plasmids can direct the production of virus with various tropisms...HIV for lentivirus). Additionally, γ-retroviruses can only transduce dividing cells because they are only... breakdown of the nuclear envelope. Lentiviruses can transduce both dividing and non-dividing cells because...plasmids or the packaging cell line. γ-Retroviruses can transduce target cells, but the transduced target...Are γ-retroviruses self-inactivating (SIN)? They can be. Plasmids that encode SIN γ-retrovirus lack most...widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted the development...
  5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...publication describing how short term storage on dry ice can shift sample pH, we wondered whether or not the dry...affected by dry ice, and specifically how dry ice can affect virus from Addgene. Bottom line: there’s ...temperatures (-80°C) and once in the tube, the CO2 can alter the pH of the liquid sample. The good news ... that this effect is reversible and the pH shift can be prevented. Keep this information in mind if you...C. For samples that are shipped on dry ice, this can affect the pH of the sample, which may have some ...Additional Resources on the Addgene Blog Learn How You Can Use the Viral Service Tips for 1st Time CRIPSR User...
  6. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...More importantly, you can use these four steps for any type of writing. You can use them to help you write...accessibility is removing barriers when we can and lowering them when we can’t. We know barriers exist in scientific...and after ultracentrifugation so that a scientist can extract virus from the correct layer.  Third, think...Where is the reader accessing the information? This can be physical or digital. For instance, you would present...results. And many journals accept alt text, so you can submit it with your manuscript and help make your...
  7. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...that lays out which experiments can be found in each folder. This can be a simple .txt document. Record...addition to output files, this is also where you can store any power point, excel, or other files relevant...the camelCaseFormat since spaces between each word can make it difficult to accurately call a file. Version... track of your exact changes. Alternatively, you can simply make a note in your methods section about ... Lab Notebook Organized Learn about Software You Can Use to Save Time and Track Your Experiments Try out...
  8. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...clone can then be monitored over time by sequencing the barcodes in the population (all barcodes can be ...of cells with specifc, easily read sequences that can be used to measure their clonal abundance before ...tumor xenograft models. She hopes that the library can be used to identify appropriate, non-overlapping ...development - such that initial resistant populations can be eradicated up-front quickly. This approach is ...25849130. 2. Hata, Aaron N., et al. "Tumor cells can follow distinct evolutionary paths to become resistant...resistant cancer cells exist prior to drug treatment. They infected a non-small cell lung cancer cell line...tool to look at a variety of questions surrounding cancer development, metastasis, and drug resistance, Bhang...
  9. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...smegmatis (Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...system for genome engineering is the fact that Cas9 can be easily programmed to make a DNA double strand ...frameshift mutation. Of some importance, the deletion can be less heterogeneous when constrained by sequence...loading of Ku and normal NHEJ activity. Other factors can also influence NHEJ activity. For example, repressing... flank regions of hundreds of base pairs or more can simultaneously introduce a pair of chromosome breaks...containing compatible overhangs is available. Cas9 can also be altered to generate a targeted single strand...and Biophysics and the Lineberger Comprehensive Cancer Center at UNC Chapel Hill.   References Bennardo...
  10. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...Additionally, you can upload your own images to the Biorender workspace. Free Biorender accounts can store 5 workspaces...color and size of icons are adjustable. New icons can be requested, although there’s no guarantee Biorender...informal settings like lab meeting presentations. You can download larger images without the watermark, which...then clicking the “Usage rights” dropdown menu, you can search for images “Labeled for reuse with modification...Google Image Search results includes images that can’t be reused without attribution, despite filtering...
Showing: 441 - 450 of 904 results