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We narrowed to 892 results for: CAN

Showing: 451 - 460 of 892 results

  1. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...binding factors (PUF). In a single step, researchers can now assemble designer PUF domains for RNA specificity...interesting because by changing certain amino acids you can change the specificity,” explained Zhanar Abil of...repressor. In principle, however, designer PUF proteins can be fused to other components to lend them many other...change the intracellular localization of RNA. “You can think of changing bases to other RNA bases or unnatural..., for example, by targeting pathways underlying cancer or other disease processes. Get the PUF Assembly...

  2. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...plants. In the most practical terms, what GreenGate can do is “save you half a year,” in comparison to more...fragments produced will have different overhangs, they can be designed such that they go back together only ... restriction endonuclease of choice, BsaI, which can be very sensitive to temperature fluctuations. He...sensitive protein,” he says. “There is nothing you can do about it but be gentle. If it no longer works,...

  3. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...within minutes of light exposure. Third, the response can be graded with light dose and is fully reversible...reversible after light retraction. Finally, because light can be applied non-invasively, its use is not restricted... endogenous cellular processes." The LITE system can now be applied to unraveling the genetic and epigenetic...time by making an AAV1 supernatant. (The details can be found here.) So tell us, readers, how will you... the brains of living mice both quickly and significantly. Genes under LITE control showed a rise in transcription...

  4. Overwhelmed? Take a Break With Our 5 Favorite Science Comics

    Type
    Blog Post
    Published
    Dec. 5, 2013, 3:27 p.m.
    ...extra push, there’s evidence that laughter really can be good for you. A search on PubMed for the word ... few fun and very recent bits of evidence. (What can I say, we are scientists). Researchers in Taiwan ...those young people in a better mood. Laughter itself can sometimes even count as demanding exercise, and, ...Taiwan showed that a laughter program significantly decreased immunological markers of stress in adolescents...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...2) which can be utilized in three successive assembly steps. Before beginning, scientists can insert fragments...lab has engineered a modular mini-Tn5 vector that can be used to generate random mutagenesis libraries ...bacterium Pseudomonas putida and demonstrate that they can successfully create GFP fusion proteins with a variety... cytochrome c oxidase subunit VIII, so that they can be imported to the mitochondrial matrix. Gottlieb...pathway or induce other compensatory mechanisms. How can protein function be dissected while minimizing confounding... a membrane-permeable pharmacological agent that can rapidly induction heterodimerization of proteins ...the final construct. Up to eight entry vectors can be used for each Multisite Gateway compatible destination...

  6. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...prime editing, whereby a multitude of configurations can be used to introduce specific target modifications...observed from high-throughput experiments, where we can evaluate tens of thousands of designs in parallel...the best pegRNA design for a specific edit, users can first define their edit in brackets and add the upstream...sequences to their input string (Figure 2). This string can then be used as input for the prediction on the website...), a model that predicts how well a genomic site can be edited based on its chromatin environment. After... pegRNA design with PRIDICT/PRIDICT2.0, ePRIDICT can help you understand how chromatin might influence...editing prediction models (such as PRIDICT/PRIDICT2.0) can greatly facilitate prime editing experiments, thus...

  7. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...Bimolecular Fluorescence Complementation (BiFC). It can provide a qualitative or quantitative readout of ...of the interaction in vivo or in vitro, and can be used for measuring protein expression or localization... When Protein A and B interact, the FP fragments can assemble the full structure of the fluorescent protein...from a different base FP (for example, EBFP2(1-10) can be used with GFP(11)). Some of the articles linked...color labeling with exogenous fluorophores, which can even be bright enough for single-molecule tracking...interest. Remember that the FP fragments themselves can sometimes interact in cells and produce background...termini are important for their interaction. You can read our previous blog post for more on BiFC principles...

  8. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...more about these two new AAV capsids and how they can be useful tools for studying the CNS and PNS! What...expressing Cre in a cell type of interest, a scientist can isolate DNA from the infected animal and use the ...this barrier - only those that cross the barrier can be identified by PCR later. The PNS presents a different...AAV-PHP.S versus their parental AAV vectors. What can these AAV’s be used for?  1. Multi-color labeling...transactivator) is co-injected. The dose of this AAV can be adjusted to achieve sparse levels of labeling,...supplemental figure 8 of Chan et al). These new AAVs can serve as tools for basic science research (neuronal...multi-color labeling of neurons (figure 4). These capsids can serve as powerful cell-type-restricted gene vectors...

  9. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...monitoring localization; using FP timers, researchers can look at a single image of a cell and understand how...correlating this ratio to the total expression time can therefore be used to determine how long a pool of...for the theoretical timer shown in Figure 1B, you can use the equation fit to this linear region to solve... one benefit of the common FP timers is that you can monitor age by expressing a single FP-timer protein... oxygen, temperature, and pH, but timer activity can be calibrated to these different conditions, enabling...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased...circuit (Saxena et al.) In a general sense, FP timers can be used in any situation where one wants to understand...

  10. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...sort of virulence as well — namely that they can cause cancer via insertional mutagenesis (which HIV does...simple spokesmodel for the whole family; however, you can use Figure 1 to compare the genomes of these two ...particle — and of the normal, replicative life cycle — can help you understand these seemingly odd choices and... of its first steps! However, retroviral vectors can still direct the translation of proteins because ...they have two dimerized linear ssRNA strands (which can have a different genomic sequence from one another...et al., 1971) and the full wild-type viral genome can direct the production, packaging, and release of ...viral particle, and how we as dedicated researchers can exploit it. This blog was written by Addgenie Brian...
Showing: 451 - 460 of 892 results