We narrowed to 904 results for: CAN

... of the AAV we have released so far in 2019. You can also browse our entire AAV inventory! Our new AAVs...fluorescence of the GCaMP7 family of calcium sensors and can be used for imaging of small neuronal processes like...Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction with optogenetic activators...AAV These fluorescent protein expression vectors can be used to visualize cells and to quantify targeting...expression to GABAergic interneurons. These vectors can also be used as the control vectors for experiments...

...victoria the functionalized nanobodies described here can be used to target any protein fused with GFP, YFP...vicinity with biotin. The biotin-labeled proteins can then be isolated and analysed. In this work, the ...School have developed a group of plasmids (here) that can be used to label all proteins within a radius of ...Tyrosine) with biotin. These biotin-labeled proteins can them be analyzed by mass spectrometry allowing a ...composition of proteins found at the telomere, and hope it can be used to expand our understanding of proteome localization...MarathonRT, isolated from Eubacterium rectale, significantly outperforms other RTs in this regard and is ...

...sequences of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both ...occurs which cuts the DNA.3.) The released transposon can now bind a strand of DNA with a TA dinucleotide (...transposon containing the gene-expression cassette that can translocate within the genome. The Sleeping Beauty...generation of stable transgenic cell lines which can robustly express your gene-of-interest, either constitutively...Additionally, in 2014 researchers at MD Anderson Cancer Center presented results from a clinical trial ...

...editing and twin prime editing can integrate DNA cargoes of 80–100 bp, PASSIGE can integrate cargoes over a ...concentration and purity of the target molecule, which can be problematic for low-abundance targets or natively...plunge freezing By Rachel Leeson Cryo-EM samples can be damaged at the air-water interface (AWI) during...small proteins that naturally adsorb to the AWI, can protect cryo-EM samples from damage (Abe & Lim, 2024...air-water interface (AWI) before vitrification, they can be completely denaturated (left) or complexes may...of dCas9 fused to a DNA methyltransferase — that can drive lasting transcriptional silencing (Nuñez et...methyltransferase for epigenetic silencing, which can be toxic, CHARM uses a cofactor fused to a histone...

...CRISPR components for plant genome engineering CRISPR can be used to knockout, activate or repress target genes...required. As with other model systems, multiple gRNAs can be expressed to modify several genomic loci at once...dCas9-VP64) or repressors (dCas9-KRAB or dCas9-SRDX) can also be used to activate or repress target genes ...result in a failed experiment. CRISPR components can be expressed stably or transiently depending on the...method and cell type in question. CRISPR components can be delivered and expressed transiently using a standard...information on Agrobacterium-mediated transformation can be found in this blog post. The pDGE Dicot Genome...transformation in plant cells. Agrobacterium tumefaciens can be used as a vehicle to deliver your gene of interest...

...How can I quickly check if the cloning of my oligo into a backbone like PX330 worked? A12: You can quickly...newest CRISPR technologies and their applications can be exhausting. A quick search for "CRISPR", short-hand...concept of the double nickase system is that you can express two different chimeric gRNAs with the Cas9... cuts 3–4 bp upstream of the PAM sequence. There can be some off-target DSBs using wildtype Cas9. The ...Tips for using homologous recombination (HR) Q7: Can you summarize HR template vector design guidelines...shorter. Q10: What is the maximum amount of DNA that can be inserted into the genome using CRISPR/Cas for ...reference for using ssODN as HR Template? A11: You can take a look at Soldner et al. (2011) which uses ssODN...

...binding factors (PUF). In a single step, researchers can now assemble designer PUF domains for RNA specificity...interesting because by changing certain amino acids you can change the specificity,” explained Zhanar Abil of...repressor. In principle, however, designer PUF proteins can be fused to other components to lend them many other...change the intracellular localization of RNA. “You can think of changing bases to other RNA bases or unnatural..., for example, by targeting pathways underlying cancer or other disease processes. Get the PUF Assembly...

...plants. In the most practical terms, what GreenGate can do is “save you half a year,” in comparison to more...fragments produced will have different overhangs, they can be designed such that they go back together only ... restriction endonuclease of choice, BsaI, which can be very sensitive to temperature fluctuations. He...sensitive protein,” he says. “There is nothing you can do about it but be gentle. If it no longer works,...

...within minutes of light exposure. Third, the response can be graded with light dose and is fully reversible...reversible after light retraction. Finally, because light can be applied non-invasively, its use is not restricted... endogenous cellular processes." The LITE system can now be applied to unraveling the genetic and epigenetic...time by making an AAV1 supernatant. (The details can be found here.) So tell us, readers, how will you... the brains of living mice both quickly and significantly. Genes under LITE control showed a rise in transcription...

...extra push, there’s evidence that laughter really can be good for you. A search on PubMed for the word ... few fun and very recent bits of evidence. (What can I say, we are scientists). Researchers in Taiwan ...those young people in a better mood. Laughter itself can sometimes even count as demanding exercise, and, ...Taiwan showed that a laughter program significantly decreased immunological markers of stress in adolescents...