We narrowed to 932 results for: Gene

...Gibson assembly, imagine that you want to insert your gene of interest into a vector with a large tag at the...by using Gibson assembly, you can insert both the gene of interest and the tag sequences into the vector...CATCH enables one-step targeted cloning of large gene clusters. Nat Commun. 2015; Sep 1;6:8101. PubMed...the J. Craig Venter Institute in 2009. Here at Addgene we added this option to our drop down menu of common...which chews back the 5’ ends of the fragment, generating long overhangs which allows the single stranded...advantage is that this process makes it easy to generate wild type and mutant constructs at the same time...primer melting temperature greater than 48℃. Both Snapgene and NEB have tools that help you design primers...

...may be the hot new way to manipulate gene expression, but other gene manipulation systems remain valuable...important ways to spatially and temporally control gene expression, especially in in vivo models, and new...at least six generations. In addition to the neuronal pThy1-Brainbow constructs, Addgene also has two ...Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0, 1.1, 2.1 plasmids...coupling of Cre-lox with FLP-FRT recombination to generate additional recombination events, and the synthesis...and sea anemone mKate2.) They then successfully generated custom antibodies to each of these proteins and...analysis. To achieve even cell labeling, they generated farnesylated derivatives of the fluorescent proteins...

...were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...among the first batch of antibodies we produced at Addgene and, as you might suspect, it has been one of our... need a second GFP antibody?  Similarities:  Generated against full length GFP and converted to recombinant...always best to have options.      Fig. 2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662... [N86/8R] page for more details. Image credit: Addgene.   Find anti-GFP [N86/8R] here!   Andrews NP...

... provide a highly versatile means of delivering genes to many different cell types with minimal immunological...effects. AAVs are used in experiments targeting genes to specific cell types in the brain, mapping neuronal...Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV Browse Optogenetics Plasmids ... to our viral vector pages on the Addgene website and on the Addgene blog to get some ideas for future...Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering Primer on Optogenetics Resources... Addgene recently attended the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This...newbies might want to get into the field. Find Optogenetics Plasmids for Use in Neuroscience Research Neuroscience...

...Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced...lentiviral vectors can be used for gene delivery Resources on Addgene.org Visit our Stem Cell Pages Find...from the patient in vitro then correct the damaged gene in the resulting iPSCs before differentiating the...PMC3987909. 6. Ban, H., et al., Efficient generation of transgene-free human induced pluripotent stem cells...Mukherjee. The ultimate goal in the field of regenerative medicine is to replace lost or damaged cells...cell is converted to a different cell type for regeneration and repair and situations where one process ...plasmids used for these methods can be found on Addgene’s stem cell page. On this page, you can also find...

...dissociate when gene transcription is complete. However, transcription of thousands of genes is constantly...store their genetic information. All of these viruses have one thing in common – they can generate dsRNA as... Here, we will cover how these structures are generated, why it’s important for cells to sense their formation...inherently double stranded, while ssRNA viruses can generate dsRNA intermediates as part of the viral replication...as long/short interspersed nuclear elements can generate dsRNA by bidirectional transcription or as inverted...inverted repeats. These elements are almost always epigenetically silenced, but dysregulation of these systems... ionizing radiation, can also result in dsRNA generation. While these molecules are clearly associated...

...dCas9 and microbial SSAPs (dCas9-SSAP) which insert genes in a cleavage free manner. The dCas9-SSAP system...Fig. 3: Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang, et...reduces the need to generate “single-use” transgenic lines. Instead, researchers can generate and maintain transgenic...each protein of interest and, for in vivo work, generate a dedicated transgenic line. Using zebrafish as... the liver.    Fig. 2: Summary of the generational evolution of the AAV capsids (left) and transduction...

...enzyme cloning uses short sticky ends to insert genes into plasmid backbones, base pairing between these...Schematic of SLIC. To start the SLIC cloning process, a gene of interest is PCR amplified to add 5′ and 3′ homology... LIC received an important update, courtesy of Addgene depositor Stephen Elledge. His new method, named...flexible. For one thing, those 5′ overhangs can be generated in several ways. One option, imprecise T4 digestion...work directly on PCR products, with 5′ overhangs generated either by annealing a mixture of products or from...regions from the target vector. 5′ overhangs can be generated by T4 polymerase (left branch); by annealing two...concentrations of insert to succeed. 5′ overhangs are generated in the linearized vector by T4 digestion; then...

...compatible approach to simultaneously clone two genes-of-interest (GOI) into two independent expression...from a different plasmid, which may lead to unequal gene dosage and high variability of protein coexpression...    Listen to this episode of Hot Plasmids! Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed...type II CRISPR systems including the popular S. pyogenes Cas9 (Rauch et al., 2017, Hynes et al., 2017)....biological tool, Niopek and colleagues turned to optogenetics. Through the use of light-sensitive protein ... “CRISPR-Cas9 activity switching via a novel optogenetic variant of AcrIIA4.” CASANOVA responds to blue...What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires...

...ion-pump encoding genes HCN1 KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction...resources on the Addgene blog: Addgene Optogenetics guide Primer on Optogenetics blog series Addgene Biosensors... light-based genetic tools for studying bioelectricity in vivo. Here are some optogenetic and GEVI tools... Aurora and Phobos optogenetic tools - A collection of bi-stable anion optogenetic channels which can ... al. "Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget ...ion-channels and pumps in developmental biology, regeneration studies, and cancer biology. I was first introduced...affect patterning and in some cases even override genetically encoded patterning. Based upon trends in studies...