We narrowed to 904 results for: CAN

...2) which can be utilized in three successive assembly steps. Before beginning, scientists can insert fragments...lab has engineered a modular mini-Tn5 vector that can be used to generate random mutagenesis libraries ...bacterium Pseudomonas putida and demonstrate that they can successfully create GFP fusion proteins with a variety... cytochrome c oxidase subunit VIII, so that they can be imported to the mitochondrial matrix. Gottlieb...pathway or induce other compensatory mechanisms. How can protein function be dissected while minimizing confounding... a membrane-permeable pharmacological agent that can rapidly induction heterodimerization of proteins ...the final construct. Up to eight entry vectors can be used for each Multisite Gateway compatible destination...

...sort of virulence as well — namely that they can cause cancer via insertional mutagenesis (which HIV does...simple spokesmodel for the whole family; however, you can use Figure 1 to compare the genomes of these two ...particle — and of the normal, replicative life cycle — can help you understand these seemingly odd choices and... of its first steps! However, retroviral vectors can still direct the translation of proteins because ...they have two dimerized linear ssRNA strands (which can have a different genomic sequence from one another...et al., 1971) and the full wild-type viral genome can direct the production, packaging, and release of ...viral particle, and how we as dedicated researchers can exploit it. This blog was written by Addgenie Brian...

...efficiently investigate gene function. CRISPR libraries can be used to knock out, inhibit or activate target ...function while nuclease-deactivated Cas9 (dCas9) can be tethered to a repressor (CRISPRi) or an activation... are subject to an increase in dsDNA breaks that can cause cell death. Thus, CRISPR inhibition (CRISPRi...In addition to inhibiting gene expression, dCas9 can also be used for transcriptional activation. Activation...genome-wide gain of function screens. These screens can help reveal the function of lowly expressed genes...Tanenbaum et al., 2014). The sgRNA or tracrRNA sequences can also be modified to include RNA domains that recruit... screen, implying that ORF and CRISPRa libraries can be used in complement to study gene function. Find...

...more about these two new AAV capsids and how they can be useful tools for studying the CNS and PNS! What...expressing Cre in a cell type of interest, a scientist can isolate DNA from the infected animal and use the ...this barrier - only those that cross the barrier can be identified by PCR later. The PNS presents a different...AAV-PHP.S versus their parental AAV vectors. What can these AAV’s be used for?  1. Multi-color labeling...transactivator) is co-injected. The dose of this AAV can be adjusted to achieve sparse levels of labeling,...supplemental figure 8 of Chan et al). These new AAVs can serve as tools for basic science research (neuronal...multi-color labeling of neurons (figure 4). These capsids can serve as powerful cell-type-restricted gene vectors...

...prime editing, whereby a multitude of configurations can be used to introduce specific target modifications...observed from high-throughput experiments, where we can evaluate tens of thousands of designs in parallel...the best pegRNA design for a specific edit, users can first define their edit in brackets and add the upstream...sequences to their input string (Figure 2). This string can then be used as input for the prediction on the website...), a model that predicts how well a genomic site can be edited based on its chromatin environment. After... pegRNA design with PRIDICT/PRIDICT2.0, ePRIDICT can help you understand how chromatin might influence...editing prediction models (such as PRIDICT/PRIDICT2.0) can greatly facilitate prime editing experiments, thus...

...monitoring localization; using FP timers, researchers can look at a single image of a cell and understand how...correlating this ratio to the total expression time can therefore be used to determine how long a pool of...for the theoretical timer shown in Figure 1B, you can use the equation fit to this linear region to solve... one benefit of the common FP timers is that you can monitor age by expressing a single FP-timer protein... oxygen, temperature, and pH, but timer activity can be calibrated to these different conditions, enabling...aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased...circuit (Saxena et al.) In a general sense, FP timers can be used in any situation where one wants to understand...

...investigator needs a standard against which the results can be compared; results from an experiment lacking the...on to learn more about the various controls that can be used for plasmid-based experiments. What are control...expression level of Gene X in the untreated cells—but can we be certain? Do we know whether the plasmid delivered...the baseline/standard against which other samples can be compared. The Empty Vector Control (mentioned ...and biological. In general, technical replicates can be thought of as "plate controls". They are NOT independent...Conversely, biological replicates ARE independent and can be thought of as "reproducibility controls". Biological...successfully transfected our cells (Figure 3), we can continue with our experiment, incorporating additional...

...RNAs. To gain this specificity, the eukaryotic RNAP can recognize and bind to specific promoter elements....cytomegalovirus Constitutive  May contain an enhancer region. Can be silenced in some cell types. EF1a General expression...and several tetracycline operators. Transcription can be turned on or off depending on what tet transactivator...Inducible with galactose; repressible with glucose  Can be used independently or together. Regulated by GAL4...polymerase, which is also controlled by lac operator. Can be induced by IPTG. Commonly found in pET vectors...in the absense of lac repressor (lacI or lacIq). Can be induced by IPTG or lactose. Leaky promoter with...gene expression  Promoter from bacteriophage lambda Can be temperature regulatable  Often paired with the...

...mismatches. However, some ligases can ligate mismatches to a significant degree, and very active ligases...dependent DNA ligase from a thermostable bacterium that can survive high temperatures (up to 95 °C) and is active...right ligase, conditions and suitable probes, LDR can reliably detect single-base differences. The original...visualized the ligation product in a gel, but we can now use fluorophore- quencher pairs or qPCR-based...ligases, such as T4 DNA Ligase, can ligate nicks containing one or more mismatches near the ligation junction...This “peeling back” of the 5 ́-phosphorylated base can be observed in the crystal structures of several ...26). Despite this higher fidelity, Taq DNA Ligase can still detectably ligate many T:G, T:T, and A:C mismatches...

... they can scan for any signs of danger. In this post we’ll focus on how fluorescent proteins can and have...incremental change of the excitation wavelength can significantly affect emission intensities from fluorescent...coming from this volume. The consequence is that you can detect signal in thick specimens. See the video below...lymph node after infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and...interactions. Even if virtually all fluorescent proteins can be used in your experiments, experience has shown...excitation wavelength (Salomonnson et al. 2012). They can also be excited efficiently at wavelengths between...the the Cre/lox recombination system. A researcher can flank fluorescent proteins in a plasmid with loxP...