We narrowed to 803 results for: Cre

...under cold temperatures, and frigid conditions decrease the potential for microbial contamination. Additionally...Acclimatisation To resolve this impasse, our team decided to create a hybrid system where we could incorporate cold-tolerance...The event also featured a seminar on “Tools to increase scientific reproducibility” by Benoit Giquel, ...

...use small, incremental changes to understand what works and what doesn't. By being creative, thoughtful...each step as you complete it. Yes, this can be incredibly tedious, but it also works, so do it anyway.  ...

...sequence (pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP ...virus, making it ideal for CRISPR pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked...site, a pair of annealed oligos can be cloned to create a chimeric guide RNA. Find pX330-U6-Chimeric_BB-CBh-hSpCas9...

...and their GPCR binding partner, Bryan Roth’s lab created an open source resource called PRESTO-TANGO (parallel...parallel receptorome expression and screening via transcriptional output - TANGO) (Kroeze et al., 2015...are ideal candidates for use in chemogenetics. To create chemogenetics receptors, GPCRs were first mutated...

...well-characterized. During this time, we’ve also created a variety of educational resources in an effort...understanding of Addgene’s customer’s needs. Categories We created a categorization of our information in order to...for promoter and serotype, among others. We also created a normalization mechanism to “collapse” variations...

...mutations through homologous recombination. To further increase the utility of CRISPR/Cas9, it will be necessary...that enhance pol III transcription, potentially increasing the levels of gRNA expression. Since tRNAs are... of multiple gRNAs targeting a single gene can increase the likelihood of gene knockout, or multiple gRNAs...

...funding and time. While institutions that focus on creative problem solving like the Howard Hughes Medical...plant research, more initiatives are needed to increase the body of knowledge. Organizations like Open...research and effectively carry out their mission of creating new forums and opportunities for open innovation...

... the world of science publishing, and an ever increasing number of journals and publishers to choose from...them needing a subscription to the journal. An increasing number of funding bodies insist that the research...is beneficial for both the referees (as they get credit for their time and effort) and the scientific community...

...publishing has a number of drawbacks, especially the increasingly growing time from submission to publication....for their efforts, inspire future research, and increase their impact. Open access and transparency Another...journal publication or an updated preprint, further increasing their findability. Now, go out there and finish...

... viewed your profile.”  The latter is much less creepy. If we’ve met before, I’d be much more likely to...borders on my first rule of networking--”If it feels creepy, don’t do it.” It’s just not a useful way to build...rules of good resume writing. Include examples of concrete accomplishments in both the technical and “plus...