We narrowed to 932 results for: Gene

...ion-pump encoding genes HCN1 KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction...resources on the Addgene blog: Addgene Optogenetics guide Primer on Optogenetics blog series Addgene Biosensors... light-based genetic tools for studying bioelectricity in vivo. Here are some optogenetic and GEVI tools... Aurora and Phobos optogenetic tools - A collection of bi-stable anion optogenetic channels which can ... al. "Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget ...ion-channels and pumps in developmental biology, regeneration studies, and cancer biology. I was first introduced...affect patterning and in some cases even override genetically encoded patterning. Based upon trends in studies...

...editing, this epigenetic strategy doesn’t induce DNA damage or permanently alter a gene product; it simply...on Prime-editing-Assisted Site-Specific Integrase Gene Editing (PASSIGE), their previously-reported technique...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases...optimizations for prime editing CHARM: A compact epigenetic silencer Recombinant antibodies for marking astrocytes...magnetic beads for scarce macromolecules in heterogeneous samples. bioRxiv 2024.01.21.576499; doi: https...loss. Ci Ji Lim’s lab found that adding Late Embryogenesis Abundant (LEA) proteins, small proteins that...with ribonucleoprotein cargo. eVLPs contain no genetic material, which ensures the editor will only be...

...cells🎵 On the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic... of CRISPR, Addgene gave to me: Cas-E-le-ven! (Cas11)🎵 On the 10th day of CRISPR, Addgene gave to me:... day of CRISPR, Addgene gave to me: RADAR from Gao🎵On the 8th day of CRISPR, Addgene gave to me: Functional...CRISPR, Addgene gave to me: updated CRISPR101 blog posts!🎵 On the 4th day of CRISPR, Addgene gave to ...let's get started!  On the 12th day of CRISPR, Addgene gave to me: Noninvasive assessment of gut function...Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing (CRISPEY!)🎵 On the 7th...7th day of CRISPR, Addgene gave to me: Drag-and-drop genome insertion, without DNA cleavage, with CRISPR-directed...

...Alkaline phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally...GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that affect autophagy (ATG) in yeast. Atg8p (“p”...fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system was developed...via the secretory pathway, and then cleaved to generate an active form of the protein. To monitor autophagy...Pho8Δ60 in the vacuole, where it is cleaved to generate an active enzyme. Pho8 phosphatase activity or.... cytosol, mitochondria), rather than tracking generic autophagy markers. It’s particularly useful for...mitochondria-targeted variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou...

...that there are approximately 950 different human genes encoding GPCRs (Takeda et al., 2002). G proteins...2002) Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520... on Addgene.org Check out Addgene's chemogenetics guide Find chemogenetics plasmids for your research...cell type. Chemogenetics is similar to optogenetics but instead of light, these genetically modified receptors...neurons and specific behaviors. Chemogenetics uses genetically engineered receptors to interact with small...are ideal candidates for use in chemogenetics. To create chemogenetics receptors, GPCRs were first mutated... about chemogenetics and a concise table of DREADDS and their activity in neurons on Addgene’s chemogenetics...

...studies for various categories, including CRISPR gene-editing, the foundations of synthetic biology, DNA... the editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also writes Codon...

...triggered by engineered nucleases." G3: Genes| Genomes| Genetics 5.3 (2015): 407-415. PubMed PMID: 25566793...off-target mutation and not an edit in the target gene. The validation process, also known as CRISPR genotyping.... 12. Osborn, Mark J., et al. "Evaluation of TCR gene editing achieved by TALENs, CRISPR/Cas9, and megaTAL...as the field seeks to generate reproducible, quality data on the role of genetic networks in biological... the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse... CRISPR experiments, many are turning to next-generation sequencing options over older assays. Biased... therapy. Low frequency off-target effects may generate irreproducible data in a research setting, but...

...for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed for the Fragmid...plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating the vector file....2024.100519 More resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: ...been looking for just the right CRISPR vectors on Addgene and found instead ones that were… pretty close,...select the components they want for each module, generate the vector file, and quickly clone their vector...Fragmid components are available for request on the Addgene website, both as individual plasmids and as the...

...you attempt to verify the starting methionine of a gene but the blastx results start the alignment at a ...unlikely to have an exact entry for your favorite gene fused to an epitope tag or fusion protein. If your... our Snapgene-powered plasmid maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid...This post was updated on Dec 4, 2017. At Addgene, we continually use the Basic Local Alignment Search...process has changed with the introduction of next generation sequencing!  Choosing a BLAST program Of the...databases, and elements of the BLAST search pages. At Addgene, we use blastn to identify any discrepancies in... viewing blastx results. When using blastx at Addgene, we use the default “Non-redundant protein sequences...

...tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen Yuan tells us about... share on our blog, please reach out to news@addgene.org - we'd love to hear from you!       ...