We narrowed to 939 results for: Gene

...compatible approach to simultaneously clone two genes-of-interest (GOI) into two independent expression...from a different plasmid, which may lead to unequal gene dosage and high variability of protein coexpression...    Listen to this episode of Hot Plasmids! Optogenetic control of CRISPR-Cas9 by CASANOVA Article contributed...type II CRISPR systems including the popular S. pyogenes Cas9 (Rauch et al., 2017, Hynes et al., 2017)....biological tool, Niopek and colleagues turned to optogenetics. Through the use of light-sensitive protein ... “CRISPR-Cas9 activity switching via a novel optogenetic variant of AcrIIA4.” CASANOVA responds to blue...What’s great about CASANOVA? It works with the S. pyogenes Cas9, dCas9-effector fusions, and xCas9, and requires...

...dissociate when gene transcription is complete. However, transcription of thousands of genes is constantly...store their genetic information. All of these viruses have one thing in common – they can generate dsRNA as... Here, we will cover how these structures are generated, why it’s important for cells to sense their formation...inherently double stranded, while ssRNA viruses can generate dsRNA intermediates as part of the viral replication...as long/short interspersed nuclear elements can generate dsRNA by bidirectional transcription or as inverted...inverted repeats. These elements are almost always epigenetically silenced, but dysregulation of these systems... ionizing radiation, can also result in dsRNA generation. While these molecules are clearly associated...

...dCas9 and microbial SSAPs (dCas9-SSAP) which insert genes in a cleavage free manner. The dCas9-SSAP system...Fig. 3: Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang, et...reduces the need to generate “single-use” transgenic lines. Instead, researchers can generate and maintain transgenic...each protein of interest and, for in vivo work, generate a dedicated transgenic line. Using zebrafish as... the liver.    Fig. 2: Summary of the generational evolution of the AAV capsids (left) and transduction...

...including how the viral genes are arranged and regulated within the plasmid backbone.  Addgene designed and tested...format, and did not alter cell viability or global gene expression in tests. It's about Time we had a tool...the human and mouse genomes and feature additional gene targets for each species. Second, they use updated...pAAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene #44361) and pAAV-hSyn-DIO-mCherry (Addgene #50459) were packaged into...into AAV using Old (Addgene #104964) or New (Addgene #232922) pAAV2/5. Average + standard deviation from...Riedel T, Moshinsky D, 2025. Addgene Report, https://doi.org/10.57733/addgene.3waajp.  These tools ...about: Highly photostable mScarlet3 variants Addgene's own AAV2 and AAV5 packaging plasmids TimeVault...

...editing, this epigenetic strategy doesn’t induce DNA damage or permanently alter a gene product; it simply...on Prime-editing-Assisted Site-Specific Integrase Gene Editing (PASSIGE), their previously-reported technique...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases...optimizations for prime editing CHARM: A compact epigenetic silencer Recombinant antibodies for marking astrocytes...magnetic beads for scarce macromolecules in heterogeneous samples. bioRxiv 2024.01.21.576499; doi: https...loss. Ci Ji Lim’s lab found that adding Late Embryogenesis Abundant (LEA) proteins, small proteins that...with ribonucleoprotein cargo. eVLPs contain no genetic material, which ensures the editor will only be...

...edits by their predicted effects on chromatin and gene regulation. These results highlight that Cas9 binding...Conceptually, PERT reframes nonsense mutations from a gene-specific problem to a stop codon–class problem, ...existing tools, accelerating progress across the gene editing field, from fundamental mechanistic insights...Kumar, S., & Mali, P. (2025). Regulatable in vivo gene expression via adaptamers. bioRxiv. https://doi.... deposition and distribution data at Addgene. Since 2012, Addgene has distributed tens of thousands of...2025). APOBEC3 mutagenesis drives therapy resistance in breast cancer. Nature Genetics, 57(6), 1452–1462...CRISPR Treatment Resources on addgene.org CRISPR Plasmids: Addgene's collection of CRISPR plasmids CRISPR...

...cells🎵 On the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic... of CRISPR, Addgene gave to me: Cas-E-le-ven! (Cas11)🎵 On the 10th day of CRISPR, Addgene gave to me:... day of CRISPR, Addgene gave to me: RADAR from Gao🎵On the 8th day of CRISPR, Addgene gave to me: Functional...CRISPR, Addgene gave to me: updated CRISPR101 blog posts!🎵 On the 4th day of CRISPR, Addgene gave to ...let's get started!  On the 12th day of CRISPR, Addgene gave to me: Noninvasive assessment of gut function...Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing (CRISPEY!)🎵 On the 7th...7th day of CRISPR, Addgene gave to me: Drag-and-drop genome insertion, without DNA cleavage, with CRISPR-directed...

...Alkaline phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally...GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that affect autophagy (ATG) in yeast. Atg8p (“p”...fluorescent biosensor that was recently deposited with Addgene by Dr. Mark Prescott. This system was developed...via the secretory pathway, and then cleaved to generate an active form of the protein. To monitor autophagy...Pho8Δ60 in the vacuole, where it is cleaved to generate an active enzyme. Pho8 phosphatase activity or.... cytosol, mitochondria), rather than tracking generic autophagy markers. It’s particularly useful for...mitochondria-targeted variants are available from Addgene. References 1. Rosado, C., Mijaljica, D., Hatzinisiriou...

...that there are approximately 950 different human genes encoding GPCRs (Takeda et al., 2002). G proteins...2002) Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520... on Addgene.org Check out Addgene's chemogenetics guide Find chemogenetics plasmids for your research...cell type. Chemogenetics is similar to optogenetics but instead of light, these genetically modified receptors...neurons and specific behaviors. Chemogenetics uses genetically engineered receptors to interact with small...are ideal candidates for use in chemogenetics. To create chemogenetics receptors, GPCRs were first mutated... about chemogenetics and a concise table of DREADDS and their activity in neurons on Addgene’s chemogenetics...

...studies for various categories, including CRISPR gene-editing, the foundations of synthetic biology, DNA... the editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also writes Codon...