We narrowed to 798 results for: KIN

...when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which is analogous...have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating expression... in length and does not require a specific PFS, making it a very flexible cleavage system. LwaCas13a is...

... of how they actually “report” as biosensors. Making aptamers glow The flexibility of aptamers means...fluorogen and increase its fluorescence, essentially mimicking GFP.  Though the aptamer, called Spinach, required... Eric J. Perkins...

...BP Reaction takes place between the attB sites flanking the insert and the attP sites of the donor vector...gene! STEP 2: Generate an Expression Clone When making the expression clone, it is important to choose...fragments, cDNA or Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of...

... month without a new, drug-resistant ‘superbug’ making headlines in one part of the world or another. ...that only infect bacterial cells), or a bacterium picking up resistance genes from it’s environment (from...resistant bacteria to over-take the population and making them more difficult to treat in the future. Patients...

...a bit of tuning before you know your system is working as expected. Finally, for each gene you want to... scorer 1.0 (Now 2.0) (Chari et al., 2015) and picking whichever guides are closest to the transcription...Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti...

...that particular region. No matter where you are looking, you can either scan the genome yourself for potential...leading to degradation that can set you back days. Working with plasmid DNA is much easier and doesn’t require...slightly different method to genotype their pups. Taking a toe, a finger, a tail tip, or a combination of...

...we are interested in circuits that mediate drug taking behavior. If a circuit reinforces behavior (activation...sense of pleasure that the animal perceives through taking a drug. The ideal behaviors to test reinforcement...amygdala to nucleus accumbens facilitates reward seeking." Nature 475.7356 (2011): 377-380. Pubmed PMID:...

...sequence (Figure 2A). Since you don’t need additional flanking sequences, shRNA plasmids are a bit smaller, with... than miRNA plasmids, which require additional flanking regions comprised of upstream and downstream genomic...between the red boxes. Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. ...

...cells of interest. In many experiments you’ll be working with a heterogeneous cell population, for example...While flow cytometry can be used for virtually all kinds of experiments and research fields, for any experiment...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

... this, similar systems have been developed for tracking various cell types and organisms and for improving...recombinase. The XFPs in Flybow are membrane-tethered, making this system useful for visualization of cellular...construct was then used to develop a system for tracking the fate of Langerhans cells. In the Ubow mouse...