We narrowed to 904 results for: CAN

...Unlike chemical indicators, these DNA constructs can be delivered to intact tissues and organs noninvasively... selecting a sensor to use for your studies, you can start by asking ‘What is the biological phenomena...concentrations in different subcellular compartments can vary greatly. Additionally, the calcium changes you...interference, and comparison between experiments can be more difficult due to variance in sensor expression...GEX or blue/green CEPIA) or through FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based... By employing various AAV serotypes, researchers can precisely direct the calcium sensor to specific tissues...delivered using viral vectors! One of the most significant advantages of viral delivery is the potential...

...make a strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes...Most of the time, that’s enough–but if it’s not, I can always take more time to check my notes!  Know your... speaking, Murphy’s law definitely applies: what can go wrong, will go wrong. The trash removal service...to,” or “It is okay if it doesn’t go perfectly,” can help. (And do remember to breathe!)   Public speaking... a chance to comfortably engage with you. If you can give a seminar, lead a journal club, or present at.... The whole process took just under a minute. I can't say the journal club was a rousing success–the response...

...assembled, the four chains make a Y-shaped protein that can be divided into the Fragment Crystallizable region...allows them to get into samples more efficiently and can also improve localization accuracy when used in super-resolution... to use Fab fragments as primary antibodies, you can make your own antibody fragments as needed.  In contrast...complex into the sample. Alternatively, Fab fragments can be used as a block in similar scenarios. By applying... here? Many of the Fab fragments described above can be used for similar applications and have similar...interchangeable, but the extra bit of heavy chain on a Fab’ can act as a convenient site for conjugation.  Finally...

...buy-in from all of Addgene’s teams. This solution can easily be implemented in an academic lab setting,...ambiguous terms. Defining words and applying annotation can be particularly useful in academic labs when creating...Clarify: Asking for clarification during a meeting can help get everyone on the same page. Others in the...meeting might also have the same question and asking can help the team identify possible points of confusion... done. But if you implement it well, here’s what can happen: More effective meetings. When everyone at...people aren’t listening to you or understanding you can be incredibly frustrating. Clarifying the words —...

...or post-mortem experiments. Fluorescent proteins can also be identified using antibodies and immunohistochemistry... always be the best option. Fluorescent proteins can interfere with fused protein function Under some ...some circumstances, the fused fluorescent proteins can be a roadblock, rather than an aid, in the expression...., 2013). We found that the fluorescent proteins can, indeed, interfere with the correct function of the...learned from these studies is that the tag protein can impact the expression of the transgene of interest...provided evidence that some fluorescent proteins can be, in some circumstances, detrimental to the expression...chemogenetic receptors used in these techniques cannot be directly visualized with fluorescence or immunohistochemistry...

...there is a clear winner, is there something that we can invest our time into learning and extract the most...CRISPR, there are two major areas where software can help. The foremost handles the design of sgRNA and...but is also a good example of how database tools can be used to aid design. These databases are still ...approximate methods. The details of their scoring function can be found in their online tool’s documentation. Since...the tool (a screen shot and overview of the table can be found above). These categories are further discussed...concern than data output as different input types can be readily converted.  Examples: “organism”, “sequence...provide results to the user in different formats. This can have a large impact on downstream results. Examples...

...properties of state-of-the-art probes, many of which you can find at Addgene. Biliverdin chromophore Near-infrared...Shemetov et al, 2017). Modern state-of-the-art NIR FPs can be used similar to GFP-like probes by supplying a...the best for working in this spectral range. You can also check that no filters and/or cold mirrors block...multiplexing (Shcherbakova et al., 2018). NIR FPs can be combined with blue-light controlled optogenetics...based on light-oxygen-voltage (LOV) domain. They can also be used in crosstalk-free three-color imaging...red-shifted light. The same NIR FPs and biosensors can be imaged at spatial scales from sub-cellular to ...the maxima of their emission spectra. These probes can be combined in two-color and multicolor NIR imaging...

...literacy as I believe people that can see and understand data themselves can come to conclusions themselves...correctness of the hypothesis. The results of experiments can help support or reject a hypothesis. Looking at the... we learn about reality. Formally, a government can be defined as a social contract citizens enter into...drop them. Scientific realities limit what policy can do and, in this way, science has always been related...not make it happen. In a time when a top official can be quoted as saying “I’m not in the job of having...Washington as well as the satellite marches. Anyone can join the marches or support the cause. For science...to contact representatives at the state level you can click on your state in this map. Finally, the march...

...target sequence. The dsRNA can be delivered as any type of small RNA and can occur via transfection or ... a variety of small RNA types to choose from and can be catered for your specific experiment. For this...siRNA) Piwi-interacting RNA (piRNA) miRNA and piRNA can be found endogenously in many cell types, while shRNA...doesn’t always cleave the mRNA — the bound miRISC can simply block translation machinery. The composition...backbones or species-specific expression plasmids. You can also bypass Dicer processing by using synthetic, ...with no tissue specificity, so in some cases they can lead to toxicity and off target effects. More recently... to create stable knockdown cells lines, and you can usually find validated shRNA sequences for your intended...

...requested AAV prep for the 2nd year running, and you can learn more about this useful, DREADD-containing AAV...recombinase under the control of the synapsin promoter. It can be used to drive Cre expression specifically in neurons...efficiently deliver genes to various brain regions. You can partially control which cells are successfully infected...interest is expressed.  A cell-type specific Cre can provide you with that control in a modular way. ...