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We narrowed to 892 results for: CAN

Showing: 471 - 480 of 892 results

  1. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...there is a clear winner, is there something that we can invest our time into learning and extract the most...CRISPR, there are two major areas where software can help. The foremost handles the design of sgRNA and...but is also a good example of how database tools can be used to aid design. These databases are still ...approximate methods. The details of their scoring function can be found in their online tool’s documentation. Since...the tool (a screen shot and overview of the table can be found above). These categories are further discussed...concern than data output as different input types can be readily converted.  Examples: “organism”, “sequence...provide results to the user in different formats. This can have a large impact on downstream results. Examples...

  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...properties of state-of-the-art probes, many of which you can find at Addgene. Biliverdin chromophore Near-infrared...Shemetov et al, 2017). Modern state-of-the-art NIR FPs can be used similar to GFP-like probes by supplying a...the best for working in this spectral range. You can also check that no filters and/or cold mirrors block...multiplexing (Shcherbakova et al., 2018). NIR FPs can be combined with blue-light controlled optogenetics...based on light-oxygen-voltage (LOV) domain. They can also be used in crosstalk-free three-color imaging...red-shifted light. The same NIR FPs and biosensors can be imaged at spatial scales from sub-cellular to ...the maxima of their emission spectra. These probes can be combined in two-color and multicolor NIR imaging...

  3. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...target sequence. The dsRNA can be delivered as any type of small RNA and can occur via transfection or ... a variety of small RNA types to choose from and can be catered for your specific experiment. For this...siRNA) Piwi-interacting RNA (piRNA) miRNA and piRNA can be found endogenously in many cell types, while shRNA...doesn’t always cleave the mRNA — the bound miRISC can simply block translation machinery. The composition...backbones or species-specific expression plasmids. You can also bypass Dicer processing by using synthetic, ...with no tissue specificity, so in some cases they can lead to toxicity and off target effects. More recently... to create stable knockdown cells lines, and you can usually find validated shRNA sequences for your intended...

  4. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...literacy as I believe people that can see and understand data themselves can come to conclusions themselves...correctness of the hypothesis. The results of experiments can help support or reject a hypothesis. Looking at the... we learn about reality. Formally, a government can be defined as a social contract citizens enter into...drop them. Scientific realities limit what policy can do and, in this way, science has always been related...not make it happen. In a time when a top official can be quoted as saying “I’m not in the job of having...Washington as well as the satellite marches. Anyone can join the marches or support the cause. For science...to contact representatives at the state level you can click on your state in this map. Finally, the march...

  5. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...requested AAV prep for the 2nd year running, and you can learn more about this useful, DREADD-containing AAV...recombinase under the control of the synapsin promoter. It can be used to drive Cre expression specifically in neurons...efficiently deliver genes to various brain regions. You can partially control which cells are successfully infected...interest is expressed.  A cell-type specific Cre can provide you with that control in a modular way. ...

  6. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...greater than 1.5. Then, using the A260 reading, you can calculate the DNA concentration. Generally, A260 ... their corresponding fluorescence. That way, you can compare the fluorescence of your sample against this...Though not the quickest way to quantify DNA, you can use the agarose gel method to not only find out how... acidic conditions and forms a blue complex that can be quantified at 595 nm. This method is time consuming...the measurements are made in the visible range and can be read with a standard ELISA reader if no other ...sensitivity at low concentrations of DNA and it can’t distinguish between DNA and RNA. See our protocol...This is something that absorbance based methods can’t tell you and you don’t need a spectrophotometer ...

  7. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...multiple AAV lots can be run in parallel and provide serotype data in minutes and the scripts  can be easily ... 100 AAV vector preparations and showed that VGS can quickly and reliably confirm the identity of AAV ...present in Cre-dependent vectors and a script that can confirm the serotype of AAV preparations.  Using ...this simplified sequencing and analysis approach can be easily incorporated into standard QC regimens ...

  8. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...Crystal violet (CV) is water soluble and can enter the peptidoglycan layer of both bacteria types. In water...on the structure of a bacteria species and how it can be used in a lab? Here we’ll be talking about the...bacteria are stained red or pink. Purpose Gram staining can be used in medical settings to determine the general...bacteria present in an infection. This information can then be used to determine effective courses of treatment...plasmid to be inserted into a specific bacterium, it can be important to consider the type of bacteria it ...bacteria, or monoderms, have a single thick peptidoglycan membrane. Common gram positive bacteria include...negative bacteria, or diderms, have a thinner peptidoglycan membrane and an outer lipid membrane. Common...

  9. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    .... The lab demonstrated that XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells...MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied for identification of essential...  After identification of ABE activity, XMAS-TREE can also be used to purify these modifications and work...with one sgRNA per operon or gene. The sgRNA pool can also target core operons in other pneumococcal strains...

  10. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...literature. I think that delivering constructive critique can help your colleagues at the receiving end, if not...open science in your peer-review Additionally, you can ask that data and resources described in the manuscript...journal you are reviewing for. The code and the data can be deposited in the public on-line repository (like...resources, like constructs or biological material, can be deposited in the specific repositories (Addgene... authors.” By addressing the comments to “you” I can easily check if I would have formulated specific ...
Showing: 471 - 480 of 892 results