We narrowed to 932 results for: Gene

...years, but lay readers may be less familiar with gene drives and their ramifications. Readers unfamiliar...in Doug Prasher, who originally isolated the GFP gene but was not included amongst the Nobel prize winners...time on Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene, with ...contributed to the steadily increasing distrust the general public has in science and scientists (as discussed...replace scientists themselves.  The sections on optogenetics and CRISPR of course address some of the ethical...has been a key driver of the technology.” As an Addgene vet of 12 years, I’m very familiar with the role...I discussed The State of Science with the five Addgene colleagues who read it with me, the consistent ...

...Sana on LinkedIn.   References 1. Yin, R., et al. "Gene expression profiling analysis of bisphenol A-induced...induces cytoskeletal disorganization, apoptosis, and gene expression disregulation on HeLa cells." Journal...Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may enter the hood...many times and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage...Second  Edition), 2012. Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...

...17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS and PNS Advanced...Neuroscientist's View Control Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression...selectively infect a genetically defined cell population. While the EnvA genetic sequences are supplied...combination with molecular tools (e.g., optogenetic or chemogenetic receptors) enables complex control of...enter cells and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged as powerful...individual neurons they infect. However, they generally are not transmitted to adjacent neurons via synapses...of the fact that RABV is an enveloped virus. In general, viral envelopes dictate infection of target cells...

... selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic ...Martin L. Yarmush. "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent...aren’t working, you can check if virus is being generated by your transfected packaging cells. This check...and transduction. Once you’ve harvested, it’s generally a good idea to titer your virus. Getting an accurate...standardize your experiments later. Stay tuned to the Addgene blog and be sure to check out our viral vector ... PMCID: PMC330758. Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...

...Medicine. He is interested in everything related to gene expression, particularly at the RNA level. He maintains...fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics” ...flux, etc). The most commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others...More examples of these biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be...proteins interact. Oligomerization: The first generations of FPs were prone to oligomerize. This may affect...More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein...

...research (check out this article in the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve...! Thank you depositors! In fact, this year, Addgene depositors quickly stepped up to make COVID-19 ... 2,300 COVID-19 related plasmids currently in Addgene’s repository which have been requested about 1,500...whether you are a depositor or not. We will have Addgene trivia (with prizes!), polls, and shout outs, and... and we’ll be featuring the work of the Addgene community. Perhaps you’ll find plasmids and new tools ...We’ll also take a look back on some fascinating Addgene history that you might not have known about. Here's...Thursday: Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude...

...Right: T1 tomato plants that inherited an edited gene to confer resistance to the herbicide chlorsulfuron...mouse monoclonal antibodies targeting the human genes and are recommended for use in immunohistochemistry...contributing to the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...indicator ONE-GO biosensors kit Antibodies for neurodegeneration research   Optimized prime editing in dicots...applicable to dicot species. In addition, they generated offspring tomato plants that inherited the genomic...single-molecule fluorescence techniques. After generating a series of single- and double-tagged constructs...

...therapeutics. Imagine deleting a problematic copy of a gene that causes a debilitating disease! This is, of ... and applications with the type I CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org...crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A "complex" system Cas3 is part of...that the cell can use to repair. Figure 3: General overview of the Cascade-Cas3 mechanism. Created... CRISPR-Transposons Additional resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection...

... your gene of interest plus two packaging plasmids (three packaging plasmids for 3rd generation lentivirus...produce adeno-associated virus type 2 vectors. Human Gene Therapy, 13(16), 1935–43. https://doi.org/10.1089...Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources...Insect cells are a less common option for the generation of viruses, though they are a popular choice ...much larger packaging capacity — up to 38 kb! Generally, insect cell production methods produce lower ...Resources and references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV...

... depending on whether the heavy and light chain genes are contained to one plasmid or if they’ve been ... L, Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293-EBNA1 Cells. Methods...Antibodies Resources on addgene.org Addgene's Antibody Purification Protocol Addgene's Video Antibody Purification...of recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and... are contained in (Randolph et. al., 2018). At Addgene, we ensure the efficacy of our antibodies through...Resources and references More resources on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...