...negative bacteria periplasm. Efforts to tag endogenous genes for proteins in these compartments using CRISPR ...tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...quantitative imaging experiments and cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing...cellular compartments other than the cytoplasm. Generally, FPs evolved in or were engineered for use in ...N-glycosylation consensus sequences in popular FPs. This generated a palette of inert green, cyan, yellow, and blue...standard FPs. The moxFPs are now available through Addgene Many thanks to our guest bloggers Erik L. Snapp...Central PMCID: PMC2150754. Resources Avaliable at Addgene Read Our Blog Post on Protein Tags Read Other ...

...the Cell Matter? AAV: A Versatile Viral Tool for Gene Expression in Mammals Recombinase-based State Machines...excellent posts from July: Addgene at the ASM Microbe 2016 Conference Generating Mouse Models Using CRISPR... them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the...perturbations to how CRISPR has made it much easier to generate mouse models.   Cpf1 Update: Comparison to Cas9... Science Communications Intern (and soon to be Addgene Scientist!) Mary Gearing, this post focuses on ...

...Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great Tools for Gene Expression...Check out Addgene’s Step-By-Step Protocol Pressed For Time? Order Ready-to-Use AAV from Addgene ...Expression in Mammals Resources on Addgene.org Find AAV Packaging and Transfer Plasmids Here New to Making...

...promoter. Use this for doxycycline inducible (tet-off) gene expression! We've also added AAV1 and AAV5 preparations...variants of a new optogenetic tool called PdCO, deposited by the Yizhar lab. Addgene is now offering AAV1... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...to our viral vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser...AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is an "optoGPCR" (coupled...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...

...Gladys will use plasmids containing a variety of HPV genes as positive controls in her PCR screens. She hopes... Sciences in Ghana will be receiving the third Addgene/Seeding Labs plasmid grant and plans to use the...future. Future awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of ...inspiring research. Additional Resources on the Addgene Blog Learn How We Raised Money for Rare Disease...Grants to Rare Disease Research Resources on Addgene.org Check out our Cancer Pages Learn about Global...

...6 letter representation of the gene (or DNA sequence). pBACKBONE-hGene Tip: Often researchers will add...pBACKBONE-Flag-hGene(Q295A)-EGFP  Wildtype version: pBACKBONE-Flag-hGene-EGFP or pBACKBONE-Flag-hGene(wt)-EGFP...insert, you would list it first. pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal... would list it after the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert contains...be included in the plasmid name. Mutations are generally listed as the amino acid change and not a nucleotide...

... Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... Senaratne is a graduate of the PhD program in Genetics and Genomics at Harvard Medical School, and is...is currently a fellow in clinical genetics at the University of California, Los Angeles. She enjoys chromosome...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...

...Prof Jim Collins and specialising in engineering gene regulation in yeast. Dr. Ellis is now one of the...Today’s episode of the Addgene Podcast features guest host and Addgene European Outreach Scientist, Benoit...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...it was a short journey for Benoit to interview Addgene Advisory Board Member and lead researcher at Imperial...Chapter 7/Outro: Tom’s Involvement with iGEM and Addgene  Many thanks to our guest, Tom Ellis! Dr. Ellis...direct interactions between drugs and promoters of oncogenes. He returned to academic research in 2005 to investigate...Additional Resources from the Addgene Blog Learn about the Synthetic Biology Education Nonprofit BioBuilder...

... Program in Genetics and Genomics at Harvard Medical School. He studies developmental gene regulation ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... Senaratne is a graduate of the PhD program in Genetics and Genomics at Harvard Medical School, and is...is currently a fellow in clinical genetics at the University of California, Los Angeles. She enjoys chromosome...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...

...Use cloning grade DNA for direct cloning of your gene of interest into a plasmid backbone. Transfection...quantity and purity.  Additional resource on the Addgene blog Browse blog posts on plasmid cloning Read... conjugation, and transfection Resources on Addgene.org Read the molecular biology reference Find plasmids...