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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 792 results for: KIN

Showing: 471 - 480 of 792 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...adds a repressible element to this binary system (making this a repressible binary system). The GAL80 repressor...PMID: 1319065; PMCID: PMC49329. Triezenberg SJ, Kingsbury RC, McKnight SL. Functional dissection of VP16... Resources on the Addgene Blog Quick guide to working with Drosophila Terminators and PolyA Signals FLEx...

  2. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...RNase III enzyme named Dicer (Figure 1). The plant kingdom has expanded its repertoire of Dicer enzymes and...silencing effect through targeted mRNA degradation, blocking translation, or chromatin modifications of complementary...researchers studying plants and other organisms lacking this innate immunity need not worry and have benefited...

  3. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... and SciFi.  Scientists around the world began looking for the magic molecule that serves as the code ...insatiable appetite of the moviegoers for dinosaurs wreaking havoc in a theme park.  Released in 1997, GATTACA... at University of California San Diego. She is working in collaboration with Prof. Alexis Komor and together...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ... a method relies on accurate ligation of nicks lacking gaps or mismatched base pairs (as in the case for....6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure, reaction...: 16464020.  3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ligases: structural and functional...

  5. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...in common? The three most important items when looking for a house and choosing an AAV are… location, .... So how do you choose the right serotype? Keep asking questions!  Table 1: AAV Serotypes and Tropism ...choice of AAV will depend upon which section you’re looking at (striatum, hippocampus and cortex) within the...

  6. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...interest and 50 nucleotides of homology to regions flanking the target site).  The best applications for dsDNA...Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald L. Court. An efficient...Read about Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR...

  7. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...the template strand. Therefore, I suggest that picking a crRNA complementary to the template strand could....For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG PAM to NGC in ...PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift in the...

  8. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...the better the detection of the target marker. Looking at Table 1, we can recognize a couple of fluorophores...brightness levels are often not that great. When thinking of terms like "very bright" vs. "dim" you might...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

  9. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...Challenge, now taking place Dec. 6, 2022! Share your AAV data, connect with other researchers working with AAV...
Showing: 471 - 480 of 792 results