We narrowed to 904 results for: CAN

...Crystal violet (CV) is water soluble and can enter the peptidoglycan layer of both bacteria types. In water...on the structure of a bacteria species and how it can be used in a lab? Here we’ll be talking about the...bacteria are stained red or pink. Purpose Gram staining can be used in medical settings to determine the general...bacteria present in an infection. This information can then be used to determine effective courses of treatment...plasmid to be inserted into a specific bacterium, it can be important to consider the type of bacteria it ...bacteria, or monoderms, have a single thick peptidoglycan membrane. Common gram positive bacteria include...negative bacteria, or diderms, have a thinner peptidoglycan membrane and an outer lipid membrane. Common...

.... The lab demonstrated that XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells...MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied for identification of essential...  After identification of ABE activity, XMAS-TREE can also be used to purify these modifications and work...with one sgRNA per operon or gene. The sgRNA pool can also target core operons in other pneumococcal strains...

...greater than 1.5. Then, using the A260 reading, you can calculate the DNA concentration. Generally, A260 ... their corresponding fluorescence. That way, you can compare the fluorescence of your sample against this...Though not the quickest way to quantify DNA, you can use the agarose gel method to not only find out how... acidic conditions and forms a blue complex that can be quantified at 595 nm. This method is time consuming...the measurements are made in the visible range and can be read with a standard ELISA reader if no other ...sensitivity at low concentrations of DNA and it can’t distinguish between DNA and RNA. See our protocol...This is something that absorbance based methods can’t tell you and you don’t need a spectrophotometer ...

...multiple AAV lots can be run in parallel and provide serotype data in minutes and the scripts  can be easily ... 100 AAV vector preparations and showed that VGS can quickly and reliably confirm the identity of AAV ...present in Cre-dependent vectors and a script that can confirm the serotype of AAV preparations.  Using ...this simplified sequencing and analysis approach can be easily incorporated into standard QC regimens ...

...literature. I think that delivering constructive critique can help your colleagues at the receiving end, if not...open science in your peer-review Additionally, you can ask that data and resources described in the manuscript...journal you are reviewing for. The code and the data can be deposited in the public on-line repository (like...resources, like constructs or biological material, can be deposited in the specific repositories (Addgene... authors.” By addressing the comments to “you” I can easily check if I would have formulated specific ...

...AAV we have released in the last few months. You can also browse our entire AAV inventory. Our new AAVs...size 20 µL samples of control AAV that scientists can use for serotype testing. These samples are available...non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson...encode engineered ligand-gated ion channels that can be activated with FDA-approved drugs. See our inventory...inventory. Flp is another useful recombinase that can be used in combination with Cre for intersectional...

...courses get plenty of theory in their lectures, but it can be difficult to determine how much information they...it in an interactive setting. The problem is…how can you make a “hands-on” lesson on genetic manipulation...let your students play. As your students play, you can observe, answer questions, and guide them to productive...! Accessing and modifying BUILD YOUR PLASMID You can find rules, suggestions, and more details on how ...teaching developmental biology Learn how BioBuilder can help teach students about Synthetic Biology Find ... various diseases. That’s when I thought, if it cannot be hands-on, it will be “brains-on”! I know that...

...but a longer term and more substantial experience can be achieved by volunteering for a professional organization...Hopefully sharing my experience will show you how you can get started on your own development as a leader. ...become Chair of a Committee overnight (however this can be done if you already have some leadership experience...sections of the NPA’s website. By volunteering, you can gain experience working with other people mostly ...gaining transferable skills such as leadership skills can only assist you and make you more qualified for future.... Becoming involved is not too difficult and you can find opportunities around you. You just need to be...

...make a pretty good team. We have confidence that we can take on any challenge and quickly become experts ...organisms, and GMOs have a bad reputation. The public is can be fearful of this technology and angry at the agriculture...hate GMOs, but what you are doing makes sense. I can support that.” That was interesting. If people could...’s exhilarating to have that sort of freedom. It can also be overwhelming – there is so much knowledge... that the learning curve is pretty steep. But we can rely on one another to celebrate good news or share... pursuing scientific or business enterprises, he can be found climbing mountains or playing with his two...

...Organism box. This small piece of information can significantly reduce your wait time for blastn, blastp, ...sequences (blastn)” program for blastn so that you can extract practically every single reliable base pair...results. This option is not as fast as megablast, but can return longer alignments to compare with your sequencing...mismatches or gaps in the nucleotide sequence, you can run a Translated BLAST (blastx) search to check for...blastx search. An alignment omitting repeated regions can be confusing, such as when you attempt to verify ...reading frame to choose for your sequencing result and can easily translate it, we recommend using blastp over...