...protected. Effects of Activation of the PGC-1a1 Pathway Gene expression analysis of the animals’ skeletal muscles...protects the brain from depression. Mice that are genetically modified to produce extra PGC-1a1 only in their...psychiatric and cognitive conditions as well as neurodegenerative diseases.” I don’t know about you, but I think...right place. Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC-1α1 modulates...

...without antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can skip this... perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We do...Preparation of electrocompetent cells (protocol) At Addgene, we use the Mix & Go! E. coli Transformation Kit...large number of transformations being performed at Addgene, this adds up to a significant time savings. You...

...supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional gene coding for heme...applications of fluorescent proteins, genetically encoded biosensors, and optogenetic tools. Follow her recent research...-the-art probes, many of which you can find at Addgene. Biliverdin chromophore Near-infrared fluorescent...FPs can be combined with blue-light controlled optogenetics, such as channelrhodopsins and tools based on...reported widely used dimeric iRFPs (Table 1). In general, the monomeric state of a probe leads to less interference... Ref BphP-based FPs miRFP670 https://www.addgene.org/79987/ 642 670 87,400 14.0 198 Monomer  155...155 4.5 72 5 miRFP703 https://www.addgene.org/79988/ 674 703 90,900 8.6 127 Monomer 394 4.5 37 ...

...aftermath of the Supreme Court’s decision to ban gene patents in a case against Myriad, an organization...should come as no surprise that those of us at Addgene believe in the power of sharing, and especially...s how science progresses after all, with each generation of scientists standing on the shoulders of their...public database to gather the results of Myriad's genetic tests for breast and ovarian cancer risk directly...And, last but not least (certainly not for us), Addgene launched this blog, A Better Way to Share Science...

...Directed Mutagenesis by PCR Google Forums Round Up: First Impressions of NgAgo CRISPR Between the Genes: How...quick way to generate many mutated plasmid constructs. The second post was contributed by Addgene Scientist...month including a first of its kind for the Addgene blog - Addgene Software Engineer Kris Shamloo wrote a ... Read All Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the...optimize and troubleshoot the system. Site Directed Mutagenesis by PCR & Single Base Editing with CRISPR For ...and discusses a newer, CRISPR-based technique to generate C->T or G->A transitions in a target sequence ...potential specificity issues, and other means of generating mutations in live cells are all discussed further...

... technique, PCR primers specific to the 16s RNA genes of various commonly infecting mycoplasmas are used...The primers used in the process are either species/gene-specific or universal. This method is quick, efficient... 93–103 Additional resources on the Addgene blog Browse Addgene's viral vectors articles Find tips and...working with viral vectors Resources on Addgene.org Check out Addgene's written and video protocols ...being contamination free. The aerosols that are generated (while talking or pipetting) during working in...leading causes of contamination. These aerosols generated will enter the culture medium during subculturing...are used which trigger cellular mechanisms that generate luminescence. This can then be detected by a luminometer...

...elegant bacterial immune system and an efficient gene editing tool… but boy does it have a lot of parts... resources on addgene.org  Addgene's CRISPR Guide Addgene's CRISPR 101 eBook  Addgene's CRISPR Cheat Sheet...section of the bacterial chromosome that stores a genetic record of previously encountered foreign nucleic...looser PAM requirements, which are available from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from...CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence...sequence as the scaffold. gRNA: "Guide RNA" is a general term referring to the RNAs used by Cas proteins...Conclusion Ready for some more details? Check out the Addgene CRISPR guide or our CRISPR 101 ebook. We also ...

...line Y, or the phenotype of a mouse with a specific gene knock out, often you have to remember the experiment...Data Sharing Resources at Addgene.org Deposit Your Plasmids with Addgene Find Molecular Biology Protocols...innovation by all.  Additional Resources on the Addgene Blog Use Apps to Save Time and Track Your Experiments...

...this publication at Addgene. A protein-tagging system for signal amplification in gene expression and fluorescence...multiple protein molecules (and also available from Addgene!) Since the signal level provided by SunTag is ...applicable to studies of cell cycle progression, epigenetics and cellular reactions to external stimuli. It...pantheon of useful CRISPR/Cas9 techniques, we at Addgene are excited to see what’s coming next! References.... Find the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human.... Find the plasmids from this publication at Addgene. ...

... GFP-containing organism,” says Hanson, “using a gene encoding one of the unexpectedly photoconvertible... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...multiple important technologies, including FRET and optogenetics. Even though GFP has been so thoroughly characterized... GFP is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used...PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis...