We narrowed to 798 results for: KIN

...interest and 50 nucleotides of homology to regions flanking the target site).  The best applications for dsDNA...Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald L. Court. An efficient...Read about Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR...

...the template strand. Therefore, I suggest that picking a crRNA complementary to the template strand could....For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG PAM to NGC in ...PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift in the...

...the better the detection of the target marker. Looking at Table 1, we can recognize a couple of fluorophores...brightness levels are often not that great. When thinking of terms like "very bright" vs. "dim" you might...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

...Challenge, now taking place Dec. 6, 2022! Share your AAV data, connect with other researchers working with AAV...

...community, we’re asking you for plasmid suggestions. Can’t find a plasmid you’re looking for in the repository...

...’s Day on the Addgene blog.  We’ve been mostly working remotely for the last year to give our lab and ...in the raffle, you will receive an email from us asking for your mailing address and T shirt size. You...

...of Addgene's winter holiday celebrations! We're kicking it off by sharing all the incredible guest posts...NEB's DADExperts: Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan...

...I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020...video channels are also underrated and I recommend checking them out, especially since videos on synthetic...

...better way to start off our holiday season than by thanking the twelve guest bloggers and subject matter experts...Toj, a summer intern who shared their experience working at Addgene. An Addgene Summer Internship Matteo...

...crystal structures demonstrating “the structure is strikingly similar to the design model at atomic resolution...That made it a powerful proof of concept that this kind of protein design is possible. Although Top7 was...