We narrowed to 605 results for: RAN-1

...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...have the same issues as one doing experiments in France. We can learn with each other through globalized...

...class.   Four ways to improve your next manuscript 1. Be consistent with your figures The figures of a ...worthless. But simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts...

...wide range of input DNA concentrations. This process is explained more fully in the animated Fig. 1 below...plasmids on a plate) will typically have a wide range of input quantities. This variation is problematic...produced from large numbers of samples with a wide range of input concentrations (via proprietary chemistry...differences in input concentration across a 100-fold range (1ng – 100ng) are normalized to two-fold variation...

...heterodimerizes with CIBN upon blue light exposure (see Fig 1*). When the group targeted the enhancer region of ...ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well as mammalian and human cell...PubMed PMID: 31026591. Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed...are also thought to play a key role in the transcriptional regulation of many genes. A number of groups...thus far for these latter experiments, such as transcription factors tethered to dCas9, require long time...

...then scroll on for some wintery delights!    Table 1: New ready-to-use viral vectors in the Addgene repository...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure...

... and can likely be used in a wide range of bacteria. References 1. Maddalo, Danilo, et al. “In vivo...post about multiplexing with Cpf1.   Figure 1: Multiplexing allows researchers to express multiple...humanized wt Cas9, dCas9 (transcriptional repressor), and dCas9-VP64 (transcriptional activator)-containing...Multiplexing from a single transcript You can also multiplex gRNAs via a polycistronic transcript. Rather than being...being transcribed from different promoters, the gRNAs are transcribed together and are flanked by specific...simultaneously. Vectors for both transient expression and Agrobacterium-mediated transformation are available. For ...system for processing a continuous multi-gRNA transcript, each gRNA must be expressed from its own promoter...

...produce as many as 700 copies per cell. Figure 1: A plasmid map showing the standard features of a ...plasmids from the same group should not be co-transformed, so if you need two plasmids for an experiment... same incompatibility group should not be co-transformed. +Actual copy number varies. See below for ...stocks, agar stabs, or liquid cultures. Freshly transformed cells also give a higher plasmid yield that colonies...

...extension. Now, they split EB1 into two halves (Fig. 1): one containing the microtubule binding domain and...manipulations on cells, and has potential use for a wide range of applications in cellular biology. Van Haren ...fluorescent proteins (FP) with improved tissue penetrance and live cell imaging capabilities. Upon developing...

...Article contributed by Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled screening using...Sabrina Büttner’s lab designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized...contributed by Jennifer Tsang Figure 4: Double stranded DNA bound by tTALE-FP with the fluorescent protein...

...net negative charge with 4 negatively charged and 1 positively charged amino acids. Myc tags are frequently...some proteins. Protein folding issues, steric hindrances, and destabilization can be brought about by ...DOI: 10.1038/521274a Arribere, J. A., et al., Translation readthrough mitigation. Nature. (2016) 534, 719...