We narrowed to 904 results for: CAN

... POI can affect protein folding and protein-protein interactions. Also, overexpression of FPs can trigger...other). Combined with AAVs, FPs can be easily expressed in the brain and can be detected through their direct...protein partners within the cell. However, antibodies can have low affinity for these tags and even multimeric...epitopes tags, FPs are intrinsically fluorescent and can therefore be used in live imaging experiments crucial...bright, stable and well tolerated by cells. They can be used for protein localization, isolation, and ...that emit across the visible spectrum, researchers can also use multiple FPs in single experiments and visualize...These probes are both intrinsically fluorescent and can be detected with highly epitope-specific, fluorescently-labeled...

...antibody can be directly conjugated with a reporter enzyme or a conjugated secondary antibody can be used...conjugated antibody. While labs can conjugate antibodies themselves, the process can be laborious and expensive...conjugation methods can interfere with binding, and some common antibody buffer components can interfere with...accuracy, specificity, and sensitivity (a good ELISA can detect picogram quantities of the target!) and use...antibodies are conjugated with a reporter or tag that can then be detected.  Typically, the reporter is an ...often opt for the chromogenic substrates since they can be read on a standard plate reader as opposed to ...saving time. Moreover, since secondary antibodies can cross-react with samples, eliminating them from the...

... time. 1D barcodes can be purchased from many companies such as Corning. Tubes can even have specific ...  If a plate of samples is ever dropped, we can simply scan the tubes and know what is in each one. Even...samples, it can be done easily with a barcode reader and an Excel spreadsheet. Start by scanning the sample...shown below). This number ensures that every sample can be tracked easily at each step in the storage process...sample at Addgene has four layers of location which can all be extrapolated from the 2D barcode and LIMS:...Tubes are assigned a location within a plate that can hold up to 96 tubes (see image below). Each plate...metal freezer rack that contains five levels that can hold five plates on each level. This rack is located...

... Basically, if you can devise an experimental assay that works with flies, you can utilize the resources... Seahorse can be employed to look at oxygen consumption. Drosophila are also used in cancer research (... I’ll provide a brief introduction as to how you can engineer new transgenic flies to study your favorite...others use attP sequence docking sites so that you can control exactly in which locus your transgene ends...1): Random insertion has the advantage that you can get varying expression levels of your transgene in...for genetic recombination so that the transgenes can be inherited on the same chromosome. Targeted Insertion...which chromosome your transgene has integrated. This can be accomplished with a few matings to balancer chromosome...

...diseases can result (Fanconi Anemia, cancer, Fragile X syndrome, etc.). Hopefully now you can appreciate...of these viruses have one thing in common – they can generate dsRNA as part of their infection and/or ... inherently double stranded, while ssRNA viruses can generate dsRNA intermediates as part of the viral...circulates, but upon viral infection, this nucleic acid can build up. The foreign dsRNA activates the innate ...typically RNA that the RNAi is designed against), RISC can repress translation and/or degrade target mRNA. ...such as long/short interspersed nuclear elements can generate dsRNA by bidirectional transcription or ...epigenetically silenced, but dysregulation of these systems can lead to local dsRNA production. In the same vein,...

...everyone can access data and drafts of manuscripts and grant applications in real-time so they can provide...Science is a global endeavor, and that can often mean that scientists who live across the country or ... these accelerate the progress of research, they can also present hurdles for the team of researchers ...disciplines or doing different tasks. But these challenges can be exaggerated when communicating across time zones... that helped make my experience run smoothly and can help other teams successfully overcome these challenges...every month is long enough that some experiments can get done and data analyzed between each meeting, ...conducting the experiments to the PIs. Remote meetings can be tricky, and it takes extra effort to make sure...

...Both of these can be increased by concentrating a viral stock. Different titering methods can reveal differences...virus solution can be obtained. 4. Enhancing virus-cell contact A variety of reagents can increase transduction..., you can check if virus is being generated by your transfected packaging cells. This check can be performed...An estimated 320,000 viruses can infect mammals. Even more abundant are the Earth’s estimated 1031 bacteriophages... viruses are everywhere and doing everything, it can be annoying when we try to use them in an experiment...which are factors in the technical challenges they can present. Many researchers find themselves plagued...titers (which is unavoidable for some vectors) you can concentrate your virus by ultracentrifugation of ...

...). While some mutations can remain stable as homozygous alleles, others cannot. Geneticists need a method...happens. Is it an evolutionarily conserved gene? Can you find an ortholog in the Drosophila genome? Continue...Continue reading and I’ll show you how Drosophila can be used to push your research in new and exciting...of seeing a phenotype since additional genes that can do the same function are less likely to exist). If...system), and the third post will describe how you can make your own mutant flies. Find Drosophila Resources...~9 days after setting up the genetic cross). You can tell when pupae are ready to hatch the next day because...their bodies will be very light in coloration and can look very plump. On the side of their abdomen will...

...online meeting can take some of that pressure off. This question and answer process can also help you ...campus even if you can’t visit. By talking with people at the institution, you can get a better sense ...the in-person portion got canceled, taking advantage of virtual meetings can still be a great opportunity... flood of interview requests and visitation days can be, at times, very overwhelming. As someone who applied...technologies that helped us navigate a global pandemic can also be the thing that provides a new perspective... applications opening. With virtual options, you can narrow down your list of schools by meeting with ... idea of that advisor’s research interests which can be helpful in creating your application to that program...

... This step can involve multiple smaller steps that all help to ensure that antibodies can access their... that are not exclusive to IF, but they can have a significant impact on the outcome of your IF experiment...bind to their targets, most commonly proteins. You can then use fluorescent microscopy to look for those...ICC are essentially just more general terms that can also refer to assays using non-fluorescent visualization...polyclonal, which means that multiple secondary molecules can bind to a single primary, resulting in signal amplification...of possible primary antibodies that exist and you can begin to see why generating fluorophore-conjugated...made in different species on a single sample, you can then use the corresponding secondary antibodies conjugated...