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We narrowed to 630 results for: Son

Showing: 41 - 50 of 630 results

  1. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...making the detection of rare events difficult. Dickinson et al. sought to create a new and efficient selection-based...Figure 1: Self-excising cassette schematic from Dickinson et al. (2015). Used with permission from the Genetics...your gene of interest into the FP-SEC vector using Gibson assembly. This construct will serve as the repair...for the targeted gene. After initial testing, Dickinson et al. tested this system across 7 genes in parallel...modification. Figure 2: Schematic from Dickinson et al. (2015) illustrating the steps to fluorescently...Society of America. Applications of this system Dickinson et al. have developed a modular system that requires...with a Self-Excising Drug Selection Cassette. Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein...

  2. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...background information. Your options for this step are sonication, which uses high-frequency sound waves to break...break up the DNA, or enzymatic digestion. Sonication provides a truly random digestion, but is harsher ...to the selective nature of the cutting. While sonication is usually the preferred method, enzymatic digestion...concentration.  Protein Capture After crosslinking and sonication or enzymatic digestion, you’re ready to incubate...availability, which could affect antibody selection. Sonication (or enzymatic digest) will also have to be optimized...fragment length for your output assay. A longer sonication time could mean needing to increase crosslinking...effects of increased sample agitation; a shorter sonication time could be an opportunity to decrease crosslinking...

  3. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...PubMed Central PMCID: PMC146043. 9. Nilsson, Stefan V., and Göran Magnusson. "Sealing of gaps in duplex DNA...gaps. For example, DNA assembly methods, such as Gibson Assembly® and NEBuilder® HiFi DNA Assembly, require...for a ligase? DNA ligases generally prefer fully Watson-Crick base-paired dsDNA substrates to those containing... base-pair size nearly indistinguishable from a Watson-Crick base pair, are joined with nearly the same...phosphate to the ligation). The structural/mechanistic reason for this differential is not known for certain,...allowed screening of all possible base combinations (Watson-Crick and mismatched) around the ligation junction....6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure, reaction...

  4. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...I first became a manager. Know your people Each person is unique and should be managed accordingly.  I...role is not to do all the work yourself anymore. Personally I found this to be particularly challenging. ...important” tasks my schedule. This was bad for 2 reasons. First, it soon became obvious that I didn’t have...accordingly -  but think of it as an investment: once the person is trained you will have more time to focus on ...identify what to delegate and to whom (make sure that person has time for it!). Then schedule a meeting to discuss...Unless you’re delegating a recurring task to a seasoned report, there should be questions! Remember that...learned to embrace them after one ended with the person in front of me saying “I’ve been doing some of ...

  5. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...the Deverman lab at the Broad Institute and the Wilson lab at the University of Pennsylvania independently...unlikely receptor for PHP.B, and how the Deverman and Wilson labs determined LY6A was a receptor used by PHP.B... PHP.B CNS permissiveness Both the Deverman and Wilson labs determined LY6A, also known as stem cell antigen... role in viral susceptibility. The Deverman and Wilson Labs independently identified LY6A as a receptor...was a likely player in PHP.B permissiveness. The Wilson Lab also looked for the gene variant responsible...require galactose and AAVR to transduce cells. The Wilson Lab generated results similar to the Deverman Lab...’s transduction of 293T cells. In addition, the Wilson Lab also tested the ability of PHP.B to transduce...

  6. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ... of your lab that you see in person, and helps improve your interpersonal relationships with people you...emailing documents to one person at a time. Just be sure there is a point person responsible for incorporating...replicates how you’d interact with team members in person, and improves overall communication.  Maintain...out if any milestones are missed. Spend time in person Presenting the results from my lab exchange...having collaborators spend time with each other in person. One possibility is to do a lab exchange, where...

  7. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...to our guest blogger Lydia Morrison from New England Biolabs. Lydia Morrison is a biochemist by training...This post was contributed by guest blogger Lydia Morrison from New England Biolabs. What is DNA assembly...multiple methods of DNA assembly available, including: Gibson Assembly®, BioBrick® Assembly, Golden Gate Assembly...Assembly, and NEBuilder® HiFi DNA Assembly. Gibson Assembly allows the production of scarless DNA constructs...opens the door for new opportunities in the field.  Gibson Assembly® is a registered trademark of Synthetic... Addgene Blog Learn More about Gateway Cloning Gibson Assembly and Other Long-Homology Based Cloning ...

  8. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab...and yet unanswered) question. Our recent paper (Richardson et al 2016) revealed some of the biophysical ...binding to the cleaved DNA duplex. Image from Richardson et al., 2016. We next wondered if we could...interaction with the Cas9-DNA duplex. Image from Richardson et al., 2016.   dCas9 enables sequence replacement...Results from our lab and others (Lin et al 2014, Richardson et al 2016, and Kim et al 2014) have shown that...transcribed sgRNA, and optional donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s ...worked on this publication:         References 1. Richardson, Christopher D., et al. "Enhancing homology-directed...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...called GMAP (Gibson assembly-based modular assembly platform). GMAP is based on the Gibson Assembly method...Parton DL, Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans C, Seeliger MA, Levinson NM, Chodera...as individual plasmids shipped as agar stabs. Iverson, et al. ACS Synth Bio. 2015. PubMed PMID: 26479688...30-40bp overlaps). To expand the capabilities of Gibson assembly and make a more modular platform, the ...context-dependent human fitness genes, the laboratory of Jason Moffat at the University of Toronto has created ...Vectors Available at Addgene: Lab Plasmids Dan Carlson RCIscript-GoldyTALEN, pC-GoldyTALEN Tom Ellis...pTAL7a, pTAL7b Michal Zurovec pBlue-TAL Nathan Lawson pJDS Series   Superglue proteins with SpyTag...

  10. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...is. If the person makes time for you, as needed, this is a strong indicator that this person cares about...limited job opportunities in academia - the main reason most people enter postdoctoral training. Similarly... the postdoctoral mentor, and at least one more person that knows you and your research well. Who could...and skilled speaker. There's a more fundamental reason to give presentations - networking! People get ...unpublished information and reagents. Part of the reason I got my first faculty position was because the...institute about me, even though I had not asked this person to write a letter of reference. The group leader...not take time off when you are a PF. Here is my personal list:   Exercise - nothing dissipates stress ...
Showing: 41 - 50 of 630 results