We narrowed to 523 results for: ATO

...blog post was written by Dr. Kutubuddin Molla, investigator at ICAR-Central National Rice Research Institute...integration), which is especially valuable for regulatory approval and public acceptance. Recently, viral...that TnpB can also function as an efficient gene activator when fused to a transcriptional activation domain...account: @Kutub_joy). Dr. Kutubuddin Molla is an investigator at the ICAR-Central National Rice Research Institute...

... vector preps expressing the Voltron2 voltage indicator, created by the Schreiter Lab and the GENIE Project... available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they...The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements in...fluorescent signal at baseline voltage), the investigators found that administering these AAVs with a dilute...

...recently was invited to attend the first Principal Investigator meeting of the NSF EDGE (Enabling Discovery ...parenting strategies and make important ecological indicators since species live in water and on land. Current...that tastes yucky (and is poisonous) for bird predators - birds eat a caterpillar once, vomit, and never... expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants. Arthropods...

...time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR ...platforms to reliably detect nucleic acids at the atomolar level. The Zhang lab has adapted natural RNase...reporter enables SHERLOCK to detect DNA or RNA with atomolar sensitivity and single base pair mismatch specificity... of HPV, HPV16 and HPV18, from human cells, at atomolar levels, within one hour. DETECTR thus has the ...

...simple as a piece of paper on the front of the incubator containing the passage number, cell line, and ...book and on the actual flasks of cells in the incubator). This means any other scientist who wishes to...The LGC Group also provides standards and has laboratories to carry our independent quality control for...culture contamination." Archives of pathology & laboratory medicine 133.9 (2009): 1463-1467. PubMed PMID...

...characteristics that make them ideal for studying in a laboratory environment. We’ve covered five model organisms...have been specifically developed to thrive in laboratory settings. One of its most attractive features...characterized genome, and is easily maintained in a laboratory environment. What sets Arabidopsis apart from.... The Norway rat, from an obnoxious pest to a laboratory pet. ELife, 9. https://doi.org/10.7554/elife.50651...

... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...

...expressing ADAR1-HiBiT were added to LgBiT-expressing hepatocytes or co-cultured across very narrow filters, complementation... of cystic fibrosis transmembrane conductance regulators (CFTR) (Kamada et al., 2023). CFTR is a membrane...cells12232741  Kamio, T., Kono, Y., Hirosuna, K., Ozato, T., Yamamoto, H., Hirasawa, A., Ennishi, D., Tomida...may underlie the heterogeneity and spread of hepatocellular carcinoma in human tissue and in vitro. Oncology...

...transcriptional repressor), and dCas9-VP64 (transcriptional activator)-containing plasmids. Each destination vector ...one. This approach is especially useful for combinatorial analyses. For example, if you were to develop...A versatile modular vector system for rapid combinatorial mammalian genetics.” Journal of Clinical Investigation...Cress, Brady F., et al. “CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9...

...pAAV-Ef1a-fDIO-ChrimsonR-tdTomato AAV9 Controls Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9...multiple serotypes pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes...

...ways scientists have adapted them for use in the laboratory. The discovery of GFP The story of how fluorescent...sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms...studying it grew. In particular, studies in the laboratory of UCSD biochemist Roger Tsien resulted in much...

...research as an NIH-funded Principle Investigator in the Kotton Laboratory and is the founding Director of ...Medicine and in the Department of Pathology and Laboratory Medicine. He attends in the Medical Intensive...

...Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the cell wall of green...forcing it out of hiding to become exposed to predators. Hungry birds spots the “caterpillar” and then... "Back from the dead; the curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." ...

...transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic stem cells with a lentiviral vector containing...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ...

...department.” Caitlin does this by acting as a facilitator between her team, the sales department, and operations...business.” Caitlin’s advice for budding science communicators: “My recommendation would be to take any opportunity...education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden Get tips...

... interpret signals from these reporters. Lack anatomical context/spatial localization: In vivo imaging...years to clinically treat enteric infection and inflammatory bowel conditions. In these experiments, arg1...abdomen. arg1 signal, however, can be overlaid onto anatomical ultrasound imaging to show where the bacteria...

...scientists. Over the course of the class, the moderator discussed individual sections of a paper, the ...quizzes and discussion on that week’s topic by the moderators. The assignments, which consisted of reading ...course was breaking up into focus groups with a facilitator. In the focus groups, we were required to critique...

...passion, and dedication to sharing epitomized by the creators of these plasmids with the first annual Blue Flame...has “literally enabled sharing with 100's of investigators worldwide. Now everything from every paper is...Flame awardee Sophien Kamoun of the Sainsbury Laboratory in Norwich, UK: “We’re very pleased to distribute...

...plasmid. Parts shown in grey are optional cis-regulatory elements.   Key elements of an AAV plasmid...plasmid harbors non-coding sequences. Other cis-regulatory elements Next let’s talk about some of the elements...Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary structure when...

...Willoughby.   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...promoter with self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al....

...Neurobiology has been working on genetically encoded indicators of calcium and other small molecules since the...imaging for beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila, C. elegans,...his team is now applying it to improve upon an indicator they developed and published in 2010 for visualizing...

...15-30 minute break. The method is named for the tomato or “Pomodoro” kitchen timer that the technique’...student.   The Pomodoro timer is literally a tomato! Image from Gerlos.   Tools for checking grammar...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...

...to create gene deletions of important unknown regulatory regions in Pseudomonas and Klebsiella spp. The...Ibadan, Nigeriaunder supervision of Dr. Akindele Oluwatosin Adeyi, University of Ibadan, Nigeria Gloria Otito...Candidate working under the supervision of Akindele Oluwatosin Adeyi at the University of Ibadan. She works ...

...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...

...histidine kinase that phosphorylates a response regulator (RR). TCS-induced responses are varied; one outcome...conditions, the chromophore activates the response regulator via phosphorylation, promoting transcription of...spread across three plasmids, with the response regulator encoded in the genome. To make the system less...

...effectively contribute as part of a team.” As a seasoned educator, Kuldell also knew that the best days in the classroom... or small, receive the BioBuilder textbook, a laboratory kit, and support during the academic year from...state-of-the-art lab embedded in LabCentral’s community of innovators that opened in August 2017.  This flexible teaching...

...sponsored the capital construction of our new BL2+ laboratory and the infrastructure capabilities to produce...our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently...helping thousands of sharing scientists from laboratories all over the world. With this reach and exposure...

...identified other advantages, including Cas13d-specific regulatory proteins and high targeting efficiency, both ...WYL domain-containing proteins may be broader regulators of Cas13d activity. This property makes WYL proteins...REPAIR Get tips for using Cas9 Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources...

...of stakeholders from industrial and commercial operators, government agencies, active researchers, as well...understanding of the societal ramifications of laboratory science, and how it may best be leveraged for...re always delighted to engage with potential collaborators and interested audiences. Please visit our team...

...in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding...effort to track down every last one of those gene regulatory proteins and the places in the genome where they... that, but the system is so flexible. Other investigators can easily adapt these vectors for use with ...