...communication should be fun and engaging for the communicator as well as the audience, so pick a mode of communication...Otherwise, you’re going to drop your project like a hot potato or burn yourself out trying to slog through unappealing... Low barrier to entry for both audience and communicators. Well-developed content creation platforms.  ...

...and debris. Affinity chromatography is a popular choice for this. Chromatography columns containing appropriate...using a desalting column or an ultrafiltration concentrator. As the name suggests, you may also concentrate...

...pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing, aka determining where...under the control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize...An AAV expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be...

...available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An...and purification, which will require additional laboratory reagents, equipment, and expertise. Final thoughts...production of the Cas9 protein and a gRNA in your laboratory, once these have been acquired, genomic editing...

...do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus HIV-1, well-known to...precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional activation from the 5’ ...trials for other diseases, including the common hematopoietic disorders sickle cell anemia and beta-thalassemia...

...anxiety by modulating the production of the pro-inflammatory cytokine interleukin IL-1 beta in the dorsal...shRNA to downregulate expression of miR124a, a regulatory RNA that negatively regulates BDNF levels. They...showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible for the...

...splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity and does not work...phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory systems have also been successfully implemented...Patrick Müller’s group at the FriedrichMiescher Laboratory of the Max Planck Society in Tübingen, Germany...

...feasible, it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged...for Cas9. However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs... https://doi.org/10.1038/nature14299  Senís E, Fatouros C, Große S, Wiedtke E, Niopek D, Mueller A-K, ...

...but not all. For example, LDLR expression in hematopoietic stem cells, T cells, and B cells is low. Infection...great at infecting neurons and cause minimal inflammatory response, but are not taken up by axon terminals...Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses...

...exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements, and ...& Bird, 2019) and is a global transcriptional regulator of thousands of genes. Studies have suggested ...). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith...

...tools and assumptions to reveal findings that the originator was oblivious to, no matter how much time they...they had. It could be argued that data generators may, in light of the policy, hold onto data longer before... available to everyone at once, including the generators. This practice doesn’t appear to harm the originating...

...Rhamnose induction relies on the transcriptional activator rhaS. To allow manipulation of bacteria without...synthetic ribosome binding site (using the online calculator from the Salis group) (Espah Borujeni et al.,...mix. Introduction of the strong transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to ...

...subunit. Using these affinity reagents, the investigators inhibited other RGD-binding integrins, individually...for cell adhesion. The results surprised the investigators, challenging the long-held belief that αVβ3 ...events and training courses on technologies and laboratory techniques related to antibody discovery. You...

...and opened a sequence file. Then I saw it, the chromatogram showing the telltale signs of a mutation introduced....3026 Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD: A Novel CRISPR/Cas9 Genome...10.1038/s41598-018-33408-9 Mizuno N, Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi...

...can manifest via research and data gathering, regulatory efforts, policy implementation, program management...company who decides how they are going pursue regulatory approval for a new product or a well-endowed ...example proposals and consider applying for their accelerator for some hands-on guidance.   Check out my webinar...

...worried that, because we were speaking out against legislators and because the march had been so well-publicized...Scientific Instrument Maker at Intellectual Ventures Laboratory. Vancouver, BC Canada 49.2827°N 123.1207°W – ...damage promotes cancer. She is also a science communicator, UBC Public Scholar, and a member of the Vancouver...

... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in .... For this reason I generally recommend that laboratories that have already attempted several CRISPR experiments...above or, even better, first-hand knowledge from laboratories employing CRISPR on the same organism could ...

...Addgene contracted with a professional scientific translator, L. Denise Roche, to edit the translation. We...  Many thanks to:  Daniel Mejías Sánchez, PhD, translator  Denise Roche, PhD, MD, editor, ldeniseroche@...

.... While certain cell types, such as principal excitatory cells and glia, have been successfully targeted...’s lab has developed a new AAV approach using regulatory elements that restrict expression to this cell...wild type SOX2 with either a SOX2-VP16 (strong activator) or SOX2-HP1 (strong repressor) fusion protein...

... Singapore. Chapter 4: Life as a new primary investigator. Coming up with new project ideas, applying ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab...

...structure is strikingly similar to the design model at atomic resolution” (Kuhlman and Dantas et al., 2003). ...). Design of a Novel Globular Protein Fold with Atomic-Level Accuracy. Science, 302(5649), 1364–1368. ...

... retrograde serotype, and a dTomato under the control of the E6 regulatory element, which targets it to...

...Cell demonstrate how PGC-1a1, a transcriptional coactivator induced in skeletal muscle by exercise and endurance...altogether. Their muscles showed an increase in inflammatory cytokines as a result of those mild stressors...

...included the Belmont Human Rights Commission and mediator for the Massachusetts small claims court summary...Oncology and the Chicago Marathon to benefit Neurofibromatosis, New England. Additional resources on the...

..., from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). Our most popular... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection...

...customizable DNA-binding domains based on transcription-activator-like effectors (TALEs) as anchors for genome engineering...and modular,” Konermann said. “Transcriptional activators are easy to expand or combine with different ...

... bit of a Twitter storm surrounding NgAgo, its creator, Han Chunyu of Hebei University of Science and ...Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science...

...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...researchers have plans to expand the library to include activators for more cancer-related pathways and more nodes...

...hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as well as...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used...

...this purpose? What led you to it? Howarth: Our collaborator Uli Schwarz-Linek at St. Andrews discovered ... are useful for mechanical study of proteins by atomic force microscopy (AFM). Maybe polyantibodies could...

...for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors ...different ways to succeed – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like...

...metadata for each screen is captured by BioGRID curators, including the cell line/tissue type, enzyme system...CRISPR database are encouraged to contact BioGRID curators at [email protected] to submit their published...

...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...

...Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases Rylan Larsen New serotype...multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] AAV1 Optogenetics Edward Boyden New serotype ...

...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

...line is known to be genetically manipulated, its anatomical origin, if cell line has ever been the target...informative. Spoiler alert: HeLa is the most common contaminator line.  For those wanting to access Cellosaurus...

...through Caitlin’s blood. “I come from a family of educators, mostly elementary school, middle school, and ...school, I actually served as the student course coordinator. With the guidance of one of the faculty members...

...For this series, I’ll interview three science communicators who work in the biotech, education, or nonprofit...job. “I am really more of a multimedia science communicator than a science writer these days. I do some ...

...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...

...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...