We narrowed to 291 results for: Fus

....” You can see how you could end up in a very confusing situation rather quickly!  The question   The...

...coding sequence feature that may be used to make fusion genes, detection needs to occur even if one or ...

...in the recipient plasmid by viewing the MCS and fusing the upstream restriction site to the forward primer...

....  Why the MTA?  The MTA process can be a bit confusing, and it’s certainly frustrating to find out your...

... for advice on her baby’s health but adamantly refused to get them vaccinated. A classmate told me that...

...environmental conditions (such as temperature, pH, fusion protein, etc...).  There are many online guides...

...abundance of a protein variant library is monitored by fusing the variants to eGFP followed by an internal ribosomal...

...collection Find empty backbones for fluorescent protein fusions Browse the fluorescent proteins plasmid kits ...

...incorrectly known as “three-parent babies,” where fused genetic material from intended parents is “rehoused...

...as wild lettuce. Aside from their use as food, infusions of L. taraxacifolia leaves are widely used to ...

... functional. Attaching the fluorescent protein fusions allowed us to image R bodies inside cells, and ...

...with a plasmid containing a fluorescent protein (fusion) there is huge variation in the fluorescence intensities...

...clarity. But, often these methods don’t agree. Confusing indeed! We've developed a new plasmid toolkit,...

...human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the human synapsin...

...molecular and cell biology techniques. What’s all the fuss? Well, ChIP’s complexities come not from the techniques...

...Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical...

... al. saw great results with CasRx and AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293...

...catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying enzyme, in this case a deaminase...

...buoyancy. These proteins allow dissolved gases to diffuse in and out but prohibit water from entering their...

...systems with limited packaging capacity. Still confused about the various tRNA expression strategies? ...

...journals and publishers to choose from, it gets very confusing for scientists writing research articles. What...

...so you end up acting it out in front of a very confused group of visiting Chinese undergrads. You get ...

...get high volumes of plasmid requests can end up refusing such requests in order to preserve time and money...

...fragment variables (scFvs) are ~27kD engineered fusion proteins similar to a Fab fragment but created ...

... is a naturally occurring RNase composed of the fusion of several Cas7 and Cas11 domains. Cas7-11 cuts...

...continuous light. ArcLight Q239 - A GEVI based on the fusion of the voltage sensing domain of a Ciona intestinalis...

...occur), deletions, mutations, in-frame protein fusions, and multiple cloning sites. In the simplest case...

...both of endocrine and dietary origin) that are diffused or actively transported from cells, traverse the...

...CRISPR targets — Sickle Cell Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which...

...activated through administered drugs that must diffuse throughout the body, the onset of receptor activation...

... vector type  Any tags, selectable markers, and fusion proteins listed for each plasmid  If the plasmids...

... In addition to generating fluorescent protein fusions, plasmids are available for tagging with epitope...

... McLellan JS (2020) Structure-based design of prefusion-stabilized SARS-CoV-2 spikes. Science 369:1501...

...complicated. These strains are more picky eaters and refuse to eat liver paste mixed with E. coli. Thus, to...

...spatial control of CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows...

...transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab...

...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor...

...heterogeneous cultures.  HeLa cells expressed a fusion of keratin with mGold (top) and mVenus (bottom)...

...Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas as a Marie Curie Fellow...

...funded? Are there any financial aspects that are confusing? During a traditional campus visit, many students...

...fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made a reliable...

... a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) together with a prime...

...to the gene of interest (or, if your protein is fused with a fluorescent protein), fluorescence microscopy...

... fly stocks and other reagents. If you’re ever confused about the best way to study YFG in flies, there...

...Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody Toolbox Resources...

...your elevator pitch where the other person seemed confused, bored, or particularly excited about? If you ...

...products into E. coli. Ligases within the E. coli then fuse together the PCR products via overhangs left over...

... initial model of this protein – but were very confused because the HNH nuclease active site wasn’t even...

...different applications such as fluorescent protein fusion studies and the generation of mutant cell lines...

...system indicates that it is very tricky to use and fussy to optimize. One reason could be the instability...